[2024-01-24 11:12:27,879] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:12:27,881] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:12:27,881] [INFO] DQC Reference Directory: /var/lib/cwl/stgbb8b54d3-ee6f-4ae0-b31d-75a40138e0d4/dqc_reference
[2024-01-24 11:12:29,352] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:12:29,352] [INFO] Task started: Prodigal
[2024-01-24 11:12:29,353] [INFO] Running command: gunzip -c /var/lib/cwl/stg0cee8e6b-e5a5-487d-8a5b-253a9306b142/GCF_019331655.1_ASM1933165v1_genomic.fna.gz | prodigal -d GCF_019331655.1_ASM1933165v1_genomic.fna/cds.fna -a GCF_019331655.1_ASM1933165v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:12:37,781] [INFO] Task succeeded: Prodigal
[2024-01-24 11:12:37,781] [INFO] Task started: HMMsearch
[2024-01-24 11:12:37,781] [INFO] Running command: hmmsearch --tblout GCF_019331655.1_ASM1933165v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgbb8b54d3-ee6f-4ae0-b31d-75a40138e0d4/dqc_reference/reference_markers.hmm GCF_019331655.1_ASM1933165v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:12:38,141] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:12:38,142] [INFO] Found 6/6 markers.
[2024-01-24 11:12:38,179] [INFO] Query marker FASTA was written to GCF_019331655.1_ASM1933165v1_genomic.fna/markers.fasta
[2024-01-24 11:12:38,180] [INFO] Task started: Blastn
[2024-01-24 11:12:38,180] [INFO] Running command: blastn -query GCF_019331655.1_ASM1933165v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbb8b54d3-ee6f-4ae0-b31d-75a40138e0d4/dqc_reference/reference_markers.fasta -out GCF_019331655.1_ASM1933165v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:12:38,817] [INFO] Task succeeded: Blastn
[2024-01-24 11:12:38,820] [INFO] Selected 8 target genomes.
[2024-01-24 11:12:38,821] [INFO] Target genome list was writen to GCF_019331655.1_ASM1933165v1_genomic.fna/target_genomes.txt
[2024-01-24 11:12:38,825] [INFO] Task started: fastANI
[2024-01-24 11:12:38,825] [INFO] Running command: fastANI --query /var/lib/cwl/stg0cee8e6b-e5a5-487d-8a5b-253a9306b142/GCF_019331655.1_ASM1933165v1_genomic.fna.gz --refList GCF_019331655.1_ASM1933165v1_genomic.fna/target_genomes.txt --output GCF_019331655.1_ASM1933165v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:12:45,971] [INFO] Task succeeded: fastANI
[2024-01-24 11:12:45,972] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgbb8b54d3-ee6f-4ae0-b31d-75a40138e0d4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:12:45,972] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgbb8b54d3-ee6f-4ae0-b31d-75a40138e0d4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:12:45,979] [INFO] Found 8 fastANI hits (5 hits with ANI > threshold)
[2024-01-24 11:12:45,980] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:12:45,980] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Acinetobacter baumannii	strain=PartI-Abaumannii-RM8376	GCA_022870045.1	470	470	type	True	99.9965	1306	1314	95	conclusive
Acinetobacter baumannii	strain=ATCC 19606	GCA_009035845.1	470	470	type	True	99.992	1309	1314	95	conclusive
Acinetobacter baumannii	strain=ATCC 19606	GCA_014116795.1	470	470	type	True	99.987	1285	1314	95	conclusive
Acinetobacter baumannii	strain=ATCC 19606	GCA_000737145.1	470	470	type	True	99.9864	1298	1314	95	conclusive
Acinetobacter baumannii	strain=ATCC 19606	GCA_020911985.1	470	470	type	True	99.9827	1309	1314	95	conclusive
Acinetobacter pittii	strain=CIP70.29	GCA_024390955.1	48296	48296	type	True	89.1233	1055	1314	95	below_threshold
Acinetobacter junii	strain=NCTC10307	GCA_900444875.1	40215	40215	type	True	80.6685	472	1314	95	below_threshold
Acinetobacter pecorum	strain=Sa1BUA6	GCA_014837015.1	2762215	2762215	type	True	78.8524	287	1314	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:12:45,982] [INFO] DFAST Taxonomy check result was written to GCF_019331655.1_ASM1933165v1_genomic.fna/tc_result.tsv
[2024-01-24 11:12:45,982] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:12:45,983] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:12:45,983] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgbb8b54d3-ee6f-4ae0-b31d-75a40138e0d4/dqc_reference/checkm_data
[2024-01-24 11:12:45,984] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:12:46,027] [INFO] Task started: CheckM
[2024-01-24 11:12:46,027] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_019331655.1_ASM1933165v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_019331655.1_ASM1933165v1_genomic.fna/checkm_input GCF_019331655.1_ASM1933165v1_genomic.fna/checkm_result
[2024-01-24 11:13:17,425] [INFO] Task succeeded: CheckM
[2024-01-24 11:13:17,426] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:13:17,453] [INFO] ===== Completeness check finished =====
[2024-01-24 11:13:17,454] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:13:17,454] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_019331655.1_ASM1933165v1_genomic.fna/markers.fasta)
[2024-01-24 11:13:17,454] [INFO] Task started: Blastn
[2024-01-24 11:13:17,454] [INFO] Running command: blastn -query GCF_019331655.1_ASM1933165v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbb8b54d3-ee6f-4ae0-b31d-75a40138e0d4/dqc_reference/reference_markers_gtdb.fasta -out GCF_019331655.1_ASM1933165v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:13:18,368] [INFO] Task succeeded: Blastn
[2024-01-24 11:13:18,372] [INFO] Selected 11 target genomes.
[2024-01-24 11:13:18,372] [INFO] Target genome list was writen to GCF_019331655.1_ASM1933165v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:13:18,382] [INFO] Task started: fastANI
[2024-01-24 11:13:18,382] [INFO] Running command: fastANI --query /var/lib/cwl/stg0cee8e6b-e5a5-487d-8a5b-253a9306b142/GCF_019331655.1_ASM1933165v1_genomic.fna.gz --refList GCF_019331655.1_ASM1933165v1_genomic.fna/target_genomes_gtdb.txt --output GCF_019331655.1_ASM1933165v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:13:28,678] [INFO] Task succeeded: fastANI
[2024-01-24 11:13:28,700] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:13:28,701] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_009759685.1	s__Acinetobacter baumannii	99.9957	1307	1314	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.86	96.67	0.89	0.80	5417	conclusive
GCF_000368085.1	s__Acinetobacter nosocomialis	91.9475	1114	1314	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.94	97.50	0.90	0.85	250	-
GCF_000368065.1	s__Acinetobacter seifertii	90.2408	1072	1314	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	96.88	96.48	0.87	0.81	81	-
GCF_002928115.1	s__Acinetobacter pittii_H	89.2101	1071	1314	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	96.46	96.15	0.91	0.87	9	-
GCF_001605885.1	s__Acinetobacter lactucae	88.5552	1066	1314	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.34	96.55	0.91	0.85	16	-
GCF_013344765.1	s__Acinetobacter lactucae_A	88.4587	877	1314	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.51	97.14	0.90	0.87	4	-
GCF_000399685.1	s__Acinetobacter pittii_E	88.4539	1055	1314	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000196795.1	s__Acinetobacter oleivorans	88.2096	1085	1314	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	96.22	95.12	0.90	0.85	40	-
GCF_000313935.1	s__Acinetobacter sp000313935	88.0022	1057	1314	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	99.37	99.37	0.94	0.94	2	-
GCF_000399665.1	s__Acinetobacter calcoaceticus_B	87.8159	1050	1314	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016508255.1	s__Acinetobacter calcoaceticus_E	87.299	1025	1314	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 11:13:28,703] [INFO] GTDB search result was written to GCF_019331655.1_ASM1933165v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:13:28,703] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:13:28,706] [INFO] DFAST_QC result json was written to GCF_019331655.1_ASM1933165v1_genomic.fna/dqc_result.json
[2024-01-24 11:13:28,707] [INFO] DFAST_QC completed!
[2024-01-24 11:13:28,707] [INFO] Total running time: 0h1m1s