[2024-01-24 11:43:42,397] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:43:42,399] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:43:42,399] [INFO] DQC Reference Directory: /var/lib/cwl/stg625c727f-4e36-429d-af5c-b498e09f5ec4/dqc_reference
[2024-01-24 11:43:43,923] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:43:43,924] [INFO] Task started: Prodigal
[2024-01-24 11:43:43,924] [INFO] Running command: gunzip -c /var/lib/cwl/stge01bc5ab-8728-4994-9f6a-793011cd9c99/GCF_019656095.1_ASM1965609v1_genomic.fna.gz | prodigal -d GCF_019656095.1_ASM1965609v1_genomic.fna/cds.fna -a GCF_019656095.1_ASM1965609v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:43:48,297] [INFO] Task succeeded: Prodigal
[2024-01-24 11:43:48,297] [INFO] Task started: HMMsearch
[2024-01-24 11:43:48,298] [INFO] Running command: hmmsearch --tblout GCF_019656095.1_ASM1965609v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg625c727f-4e36-429d-af5c-b498e09f5ec4/dqc_reference/reference_markers.hmm GCF_019656095.1_ASM1965609v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:43:48,578] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:43:48,579] [INFO] Found 6/6 markers.
[2024-01-24 11:43:48,598] [INFO] Query marker FASTA was written to GCF_019656095.1_ASM1965609v1_genomic.fna/markers.fasta
[2024-01-24 11:43:48,598] [INFO] Task started: Blastn
[2024-01-24 11:43:48,598] [INFO] Running command: blastn -query GCF_019656095.1_ASM1965609v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg625c727f-4e36-429d-af5c-b498e09f5ec4/dqc_reference/reference_markers.fasta -out GCF_019656095.1_ASM1965609v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:43:49,203] [INFO] Task succeeded: Blastn
[2024-01-24 11:43:49,207] [INFO] Selected 14 target genomes.
[2024-01-24 11:43:49,207] [INFO] Target genome list was writen to GCF_019656095.1_ASM1965609v1_genomic.fna/target_genomes.txt
[2024-01-24 11:43:49,230] [INFO] Task started: fastANI
[2024-01-24 11:43:49,230] [INFO] Running command: fastANI --query /var/lib/cwl/stge01bc5ab-8728-4994-9f6a-793011cd9c99/GCF_019656095.1_ASM1965609v1_genomic.fna.gz --refList GCF_019656095.1_ASM1965609v1_genomic.fna/target_genomes.txt --output GCF_019656095.1_ASM1965609v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:43:55,400] [INFO] Task succeeded: fastANI
[2024-01-24 11:43:55,400] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg625c727f-4e36-429d-af5c-b498e09f5ec4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:43:55,400] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg625c727f-4e36-429d-af5c-b498e09f5ec4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:43:55,420] [INFO] Found 13 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 11:43:55,420] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:43:55,420] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Leuconostoc rapi	strain=DSM 27776	GCA_019656095.1	1406906	1406906	type	True	100.0	626	627	95	conclusive
Leuconostoc rapi	strain=DSM 27776	GCA_016908715.1	1406906	1406906	type	True	99.9999	624	627	95	conclusive
Leuconostoc kimchii	strain=IMSNU 11154	GCA_000092505.1	136609	136609	type	True	89.3654	546	627	95	below_threshold
Leuconostoc inhae	strain=DSM 15101	GCA_019656015.1	178001	178001	suspected-type	True	82.2524	376	627	95	below_threshold
Leuconostoc miyukkimchii	strain=JCM 17445	GCA_019656055.1	910540	910540	type	True	80.2998	305	627	95	below_threshold
Leuconostoc gelidum subsp. gasicomitatum	strain=NBRC 113245	GCA_019655955.1	115778	1244	type	True	80.2897	316	627	95	below_threshold
Leuconostoc gelidum subsp. gasicomitatum	strain=type strain: LMG 18811	GCA_000196855.1	115778	1244	type	True	80.2855	318	627	95	below_threshold
Leuconostoc gelidum subsp. gelidum	strain=KCTC 3527	GCA_000166715.2	1607839	1244	type	True	80.1458	330	627	95	below_threshold
Leuconostoc gelidum subsp. aenigmaticum	strain=DSM 19375	GCA_019655935.1	1607837	1244	type	True	80.058	335	627	95	below_threshold
Leuconostoc gelidum subsp. aenigmaticum	strain=DSM 19375	GCA_020089505.1	1607837	1244	type	True	79.9965	343	627	95	below_threshold
Leuconostoc holzapfelii	strain=CCUG 54536	GCA_012396485.1	434464	434464	type	True	79.3199	174	627	95	below_threshold
Leuconostoc carnosum	strain=JCM 9695	GCA_019655915.1	1252	1252	type	True	79.1488	222	627	95	below_threshold
Weissella sagaensis	strain=X0750	GCA_005405285.1	2559928	2559928	type	True	78.0445	52	627	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:43:55,422] [INFO] DFAST Taxonomy check result was written to GCF_019656095.1_ASM1965609v1_genomic.fna/tc_result.tsv
[2024-01-24 11:43:55,423] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:43:55,423] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:43:55,423] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg625c727f-4e36-429d-af5c-b498e09f5ec4/dqc_reference/checkm_data
[2024-01-24 11:43:55,424] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:43:55,447] [INFO] Task started: CheckM
[2024-01-24 11:43:55,447] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_019656095.1_ASM1965609v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_019656095.1_ASM1965609v1_genomic.fna/checkm_input GCF_019656095.1_ASM1965609v1_genomic.fna/checkm_result
[2024-01-24 11:44:17,409] [INFO] Task succeeded: CheckM
[2024-01-24 11:44:17,410] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:44:17,430] [INFO] ===== Completeness check finished =====
[2024-01-24 11:44:17,430] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:44:17,431] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_019656095.1_ASM1965609v1_genomic.fna/markers.fasta)
[2024-01-24 11:44:17,431] [INFO] Task started: Blastn
[2024-01-24 11:44:17,431] [INFO] Running command: blastn -query GCF_019656095.1_ASM1965609v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg625c727f-4e36-429d-af5c-b498e09f5ec4/dqc_reference/reference_markers_gtdb.fasta -out GCF_019656095.1_ASM1965609v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:44:18,206] [INFO] Task succeeded: Blastn
[2024-01-24 11:44:18,209] [INFO] Selected 15 target genomes.
[2024-01-24 11:44:18,209] [INFO] Target genome list was writen to GCF_019656095.1_ASM1965609v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:44:18,221] [INFO] Task started: fastANI
[2024-01-24 11:44:18,222] [INFO] Running command: fastANI --query /var/lib/cwl/stge01bc5ab-8728-4994-9f6a-793011cd9c99/GCF_019656095.1_ASM1965609v1_genomic.fna.gz --refList GCF_019656095.1_ASM1965609v1_genomic.fna/target_genomes_gtdb.txt --output GCF_019656095.1_ASM1965609v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:44:23,597] [INFO] Task succeeded: fastANI
[2024-01-24 11:44:23,619] [INFO] Found 12 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:44:23,619] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_016908715.1	s__Leuconostoc rapi	99.9999	624	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_000092505.1	s__Leuconostoc kimchii	89.3403	548	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	99.55	99.53	0.95	0.95	3	-
GCA_000166735.2	s__Leuconostoc inhae	80.3039	270	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	98.95	98.13	0.88	0.76	13	-
GCF_000166715.1	s__Leuconostoc gelidum	80.1287	331	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	98.91	98.75	0.91	0.89	3	-
GCF_014207505.1	s__Leuconostoc carnosum	79.8688	237	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	99.31	98.70	0.92	0.87	17	-
GCF_009676745.1	s__Leuconostoc mesenteroides_B	79.8196	168	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	100.00	100.00	0.99	0.99	2	-
GCF_007954625.1	s__Leuconostoc lactis_A	79.6149	185	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	97.86	96.88	0.92	0.91	7	-
GCF_012396485.1	s__Leuconostoc holzapfelii	79.2627	174	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004354555.1	s__Leuconostoc citreum	79.1352	223	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	99.02	98.68	0.92	0.88	33	-
GCF_008107645.1	s__Leuconostoc litchii	78.9293	162	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900554745.1	s__Leuconostoc sp900554745	78.4001	92	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003096575.1	s__Convivina intestini	77.6992	71	627	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Convivina	95.0	99.99	99.99	0.99	0.99	2	-
--------------------------------------------------------------------------------
[2024-01-24 11:44:23,621] [INFO] GTDB search result was written to GCF_019656095.1_ASM1965609v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:44:23,622] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:44:23,626] [INFO] DFAST_QC result json was written to GCF_019656095.1_ASM1965609v1_genomic.fna/dqc_result.json
[2024-01-24 11:44:23,626] [INFO] DFAST_QC completed!
[2024-01-24 11:44:23,626] [INFO] Total running time: 0h0m41s