[2024-01-24 15:18:37,915] [INFO] DFAST_QC pipeline started.
[2024-01-24 15:18:37,918] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 15:18:37,919] [INFO] DQC Reference Directory: /var/lib/cwl/stged6cfb13-f4bd-4c9b-b177-4527b0d87efc/dqc_reference
[2024-01-24 15:18:39,331] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 15:18:39,332] [INFO] Task started: Prodigal
[2024-01-24 15:18:39,332] [INFO] Running command: gunzip -c /var/lib/cwl/stg833b2a9f-a470-4209-80c1-3069b158e8fe/GCF_019669905.1_ASM1966990v1_genomic.fna.gz | prodigal -d GCF_019669905.1_ASM1966990v1_genomic.fna/cds.fna -a GCF_019669905.1_ASM1966990v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 15:18:56,284] [INFO] Task succeeded: Prodigal
[2024-01-24 15:18:56,284] [INFO] Task started: HMMsearch
[2024-01-24 15:18:56,284] [INFO] Running command: hmmsearch --tblout GCF_019669905.1_ASM1966990v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stged6cfb13-f4bd-4c9b-b177-4527b0d87efc/dqc_reference/reference_markers.hmm GCF_019669905.1_ASM1966990v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 15:18:56,784] [INFO] Task succeeded: HMMsearch
[2024-01-24 15:18:56,785] [INFO] Found 6/6 markers.
[2024-01-24 15:18:56,829] [INFO] Query marker FASTA was written to GCF_019669905.1_ASM1966990v1_genomic.fna/markers.fasta
[2024-01-24 15:18:56,829] [INFO] Task started: Blastn
[2024-01-24 15:18:56,830] [INFO] Running command: blastn -query GCF_019669905.1_ASM1966990v1_genomic.fna/markers.fasta -db /var/lib/cwl/stged6cfb13-f4bd-4c9b-b177-4527b0d87efc/dqc_reference/reference_markers.fasta -out GCF_019669905.1_ASM1966990v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 15:18:57,706] [INFO] Task succeeded: Blastn
[2024-01-24 15:18:57,710] [INFO] Selected 12 target genomes.
[2024-01-24 15:18:57,710] [INFO] Target genome list was writen to GCF_019669905.1_ASM1966990v1_genomic.fna/target_genomes.txt
[2024-01-24 15:18:57,714] [INFO] Task started: fastANI
[2024-01-24 15:18:57,714] [INFO] Running command: fastANI --query /var/lib/cwl/stg833b2a9f-a470-4209-80c1-3069b158e8fe/GCF_019669905.1_ASM1966990v1_genomic.fna.gz --refList GCF_019669905.1_ASM1966990v1_genomic.fna/target_genomes.txt --output GCF_019669905.1_ASM1966990v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 15:19:10,380] [INFO] Task succeeded: fastANI
[2024-01-24 15:19:10,380] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stged6cfb13-f4bd-4c9b-b177-4527b0d87efc/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 15:19:10,380] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stged6cfb13-f4bd-4c9b-b177-4527b0d87efc/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 15:19:10,390] [INFO] Found 11 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 15:19:10,391] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 15:19:10,391] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Methylomonas koyamae	strain=Fw12E-Y	GCA_019669905.1	702114	702114	suspected-type	True	100.0	1697	1700	95	conclusive
Methylomonas koyamae	strain=JCM 16701	GCA_001312005.1	702114	702114	suspected-type	True	99.7505	1451	1700	95	conclusive
Methylomonas denitrificans	strain=FJG1	GCA_000785705.2	1538553	1538553	type	True	80.1661	745	1700	95	below_threshold
Methylomonas albis	strain=EbA	GCA_014850955.1	1854563	1854563	type	True	79.5351	675	1700	95	below_threshold
Methylomonas rhizoryzae	strain=GJ1	GCA_008632455.1	2608981	2608981	type	True	79.1814	430	1700	95	below_threshold
Methylomarinum vadi	strain=IT-4	GCA_000733935.1	438855	438855	type	True	77.3398	225	1700	95	below_threshold
Methylomicrobium lacus	strain=LW14	GCA_000527095.1	136992	136992	type	True	77.1992	254	1700	95	below_threshold
Pseudomonas bharatica	strain=CSV86	GCA_000319305.2	2692112	2692112	type	True	76.5109	81	1700	95	below_threshold
Methylobacter tundripaludum	strain=SV96	GCA_000190755.3	173365	173365	type	True	76.4478	172	1700	95	below_threshold
Serratia bockelmannii	strain=S3	GCA_008011855.1	2703793	2703793	type	True	75.4584	61	1700	95	below_threshold
Pseudomonas capeferrum	strain=WCS358	GCA_022548835.1	1495066	1495066	type	True	75.26	74	1700	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 15:19:10,393] [INFO] DFAST Taxonomy check result was written to GCF_019669905.1_ASM1966990v1_genomic.fna/tc_result.tsv
[2024-01-24 15:19:10,393] [INFO] ===== Taxonomy check completed =====
[2024-01-24 15:19:10,394] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 15:19:10,394] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stged6cfb13-f4bd-4c9b-b177-4527b0d87efc/dqc_reference/checkm_data
[2024-01-24 15:19:10,395] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 15:19:10,445] [INFO] Task started: CheckM
[2024-01-24 15:19:10,445] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_019669905.1_ASM1966990v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_019669905.1_ASM1966990v1_genomic.fna/checkm_input GCF_019669905.1_ASM1966990v1_genomic.fna/checkm_result
[2024-01-24 15:19:58,260] [INFO] Task succeeded: CheckM
[2024-01-24 15:19:58,261] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 96.50%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 15:19:58,283] [INFO] ===== Completeness check finished =====
[2024-01-24 15:19:58,284] [INFO] ===== Start GTDB Search =====
[2024-01-24 15:19:58,284] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_019669905.1_ASM1966990v1_genomic.fna/markers.fasta)
[2024-01-24 15:19:58,285] [INFO] Task started: Blastn
[2024-01-24 15:19:58,285] [INFO] Running command: blastn -query GCF_019669905.1_ASM1966990v1_genomic.fna/markers.fasta -db /var/lib/cwl/stged6cfb13-f4bd-4c9b-b177-4527b0d87efc/dqc_reference/reference_markers_gtdb.fasta -out GCF_019669905.1_ASM1966990v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 15:19:59,595] [INFO] Task succeeded: Blastn
[2024-01-24 15:19:59,598] [INFO] Selected 13 target genomes.
[2024-01-24 15:19:59,598] [INFO] Target genome list was writen to GCF_019669905.1_ASM1966990v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 15:19:59,609] [INFO] Task started: fastANI
[2024-01-24 15:19:59,609] [INFO] Running command: fastANI --query /var/lib/cwl/stg833b2a9f-a470-4209-80c1-3069b158e8fe/GCF_019669905.1_ASM1966990v1_genomic.fna.gz --refList GCF_019669905.1_ASM1966990v1_genomic.fna/target_genomes_gtdb.txt --output GCF_019669905.1_ASM1966990v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 15:20:11,647] [INFO] Task succeeded: fastANI
[2024-01-24 15:20:11,664] [INFO] Found 13 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 15:20:11,664] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_001312005.1	s__Methylomonas koyamae	99.7505	1451	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	96.46	95.49	0.87	0.86	5	conclusive
GCF_006483455.1	s__Methylomonas koyamae_B	86.8619	1131	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016865255.1	s__Methylomonas sp000702925	80.521	760	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	97.47	95.82	0.92	0.86	5	-
GCF_001644025.1	s__Methylomonas koyamae_A	80.4055	678	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	97.40	97.36	0.85	0.85	3	-
GCF_903064685.1	s__Methylomonas sp903064685	80.3553	776	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	100.00	100.00	1.00	1.00	2	-
GCF_001644035.1	s__Methylomonas methanica_A	80.0424	693	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	95.96	95.96	0.85	0.85	2	-
GCF_015711015.1	s__Methylomonas sp015711015	79.6096	547	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_903064715.1	s__Methylomonas sp903064715	79.583	677	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	100.00	100.00	1.00	1.00	2	-
GCF_008632455.1	s__Methylomonas rhizoryzae	79.138	427	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018335995.1	s__Methylomonas sp018335995	78.9928	385	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000214665.1	s__Methylomonas methanica_B	78.4119	435	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_903918375.1	s__Methylomonas sp903918375	77.1863	145	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	98.06	95.20	0.87	0.72	8	-
GCA_903931355.1	s__Methylomonas sp903931355	76.2137	98	1700	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Methylococcales;f__Methylomonadaceae;g__Methylomonas	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 15:20:11,666] [INFO] GTDB search result was written to GCF_019669905.1_ASM1966990v1_genomic.fna/result_gtdb.tsv
[2024-01-24 15:20:11,666] [INFO] ===== GTDB Search completed =====
[2024-01-24 15:20:11,669] [INFO] DFAST_QC result json was written to GCF_019669905.1_ASM1966990v1_genomic.fna/dqc_result.json
[2024-01-24 15:20:11,670] [INFO] DFAST_QC completed!
[2024-01-24 15:20:11,670] [INFO] Total running time: 0h1m34s