[2024-01-24 13:40:34,009] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:40:34,012] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:40:34,012] [INFO] DQC Reference Directory: /var/lib/cwl/stgd803a2ea-6f28-4d43-b82a-321f93eed8ee/dqc_reference
[2024-01-24 13:40:35,367] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:40:35,369] [INFO] Task started: Prodigal
[2024-01-24 13:40:35,369] [INFO] Running command: gunzip -c /var/lib/cwl/stg6a2ea579-a5a5-40e8-8d3d-ca30a05c5d5a/GCF_020176155.1_ASM2017615v1_genomic.fna.gz | prodigal -d GCF_020176155.1_ASM2017615v1_genomic.fna/cds.fna -a GCF_020176155.1_ASM2017615v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:40:43,959] [INFO] Task succeeded: Prodigal
[2024-01-24 13:40:43,959] [INFO] Task started: HMMsearch
[2024-01-24 13:40:43,959] [INFO] Running command: hmmsearch --tblout GCF_020176155.1_ASM2017615v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgd803a2ea-6f28-4d43-b82a-321f93eed8ee/dqc_reference/reference_markers.hmm GCF_020176155.1_ASM2017615v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:40:44,352] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:40:44,353] [INFO] Found 6/6 markers.
[2024-01-24 13:40:44,397] [INFO] Query marker FASTA was written to GCF_020176155.1_ASM2017615v1_genomic.fna/markers.fasta
[2024-01-24 13:40:44,397] [INFO] Task started: Blastn
[2024-01-24 13:40:44,397] [INFO] Running command: blastn -query GCF_020176155.1_ASM2017615v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd803a2ea-6f28-4d43-b82a-321f93eed8ee/dqc_reference/reference_markers.fasta -out GCF_020176155.1_ASM2017615v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:40:44,971] [INFO] Task succeeded: Blastn
[2024-01-24 13:40:44,974] [INFO] Selected 13 target genomes.
[2024-01-24 13:40:44,974] [INFO] Target genome list was writen to GCF_020176155.1_ASM2017615v1_genomic.fna/target_genomes.txt
[2024-01-24 13:40:44,979] [INFO] Task started: fastANI
[2024-01-24 13:40:44,979] [INFO] Running command: fastANI --query /var/lib/cwl/stg6a2ea579-a5a5-40e8-8d3d-ca30a05c5d5a/GCF_020176155.1_ASM2017615v1_genomic.fna.gz --refList GCF_020176155.1_ASM2017615v1_genomic.fna/target_genomes.txt --output GCF_020176155.1_ASM2017615v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:40:57,956] [INFO] Task succeeded: fastANI
[2024-01-24 13:40:57,957] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgd803a2ea-6f28-4d43-b82a-321f93eed8ee/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:40:57,957] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgd803a2ea-6f28-4d43-b82a-321f93eed8ee/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:40:57,966] [INFO] Found 10 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 13:40:57,966] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:40:57,966] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Clostridium bowmanii	strain=DSM 14206	GCA_020176155.1	132925	132925	type	True	100.0	1580	1581	95	conclusive
Clostridium bowmanii	strain=DSM 14206	GCA_018861315.1	132925	132925	type	True	99.9864	1534	1581	95	conclusive
Clostridium tagluense	strain=A121	GCA_003865095.1	360422	360422	type	True	84.5651	953	1581	95	below_threshold
Clostridium algoriphilum	strain=DSM 16153	GCA_020443705.1	198347	198347	type	True	81.3823	696	1581	95	below_threshold
Clostridium lacusfryxellense	strain=DSM 14205	GCA_018861735.1	205328	205328	type	True	81.0908	776	1581	95	below_threshold
Clostridium frigoris	strain=DSM 14204	GCA_018861865.1	205327	205327	type	True	80.9922	686	1581	95	below_threshold
Clostridium estertheticum subsp. estertheticum	strain=DSM 8809	GCA_001877035.1	1552	238834	type	True	80.8536	699	1581	95	below_threshold
Clostridium psychrophilum	strain=DSM 14207	GCA_018861705.1	132926	132926	type	True	80.8191	636	1581	95	below_threshold
Clostridium muellerianum	strain=P21	GCA_012926525.1	2716538	2716538	type	True	76.3334	198	1581	95	below_threshold
Clostridium puniceum	strain=DSM 2619	GCA_002006345.1	29367	29367	type	True	75.9649	154	1581	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:40:57,968] [INFO] DFAST Taxonomy check result was written to GCF_020176155.1_ASM2017615v1_genomic.fna/tc_result.tsv
[2024-01-24 13:40:57,969] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:40:57,969] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:40:57,969] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgd803a2ea-6f28-4d43-b82a-321f93eed8ee/dqc_reference/checkm_data
[2024-01-24 13:40:57,970] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:40:58,020] [INFO] Task started: CheckM
[2024-01-24 13:40:58,020] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_020176155.1_ASM2017615v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_020176155.1_ASM2017615v1_genomic.fna/checkm_input GCF_020176155.1_ASM2017615v1_genomic.fna/checkm_result
[2024-01-24 13:41:30,452] [INFO] Task succeeded: CheckM
[2024-01-24 13:41:30,453] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:41:30,480] [INFO] ===== Completeness check finished =====
[2024-01-24 13:41:30,480] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:41:30,481] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_020176155.1_ASM2017615v1_genomic.fna/markers.fasta)
[2024-01-24 13:41:30,481] [INFO] Task started: Blastn
[2024-01-24 13:41:30,481] [INFO] Running command: blastn -query GCF_020176155.1_ASM2017615v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd803a2ea-6f28-4d43-b82a-321f93eed8ee/dqc_reference/reference_markers_gtdb.fasta -out GCF_020176155.1_ASM2017615v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:41:31,302] [INFO] Task succeeded: Blastn
[2024-01-24 13:41:31,305] [INFO] Selected 8 target genomes.
[2024-01-24 13:41:31,305] [INFO] Target genome list was writen to GCF_020176155.1_ASM2017615v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:41:31,313] [INFO] Task started: fastANI
[2024-01-24 13:41:31,313] [INFO] Running command: fastANI --query /var/lib/cwl/stg6a2ea579-a5a5-40e8-8d3d-ca30a05c5d5a/GCF_020176155.1_ASM2017615v1_genomic.fna.gz --refList GCF_020176155.1_ASM2017615v1_genomic.fna/target_genomes_gtdb.txt --output GCF_020176155.1_ASM2017615v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:41:40,739] [INFO] Task succeeded: fastANI
[2024-01-24 13:41:40,747] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:41:40,747] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_018861315.1	s__Clostridium_AD bowmanii	99.9864	1534	1581	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium_AD	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_011065935.1	s__Clostridium_AD estertheticum_A	84.8864	993	1581	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium_AD	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018861755.1	s__Clostridium_AD sp018861755	84.8034	1003	1581	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium_AD	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003865095.1	s__Clostridium_AD tagluense	84.5519	955	1581	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium_AD	95.0	96.49	95.92	0.80	0.78	4	-
GCF_011065905.1	s__Clostridium_AD estertheticum_B	82.9557	704	1581	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium_AD	95.0	97.19	97.19	0.83	0.83	2	-
GCA_016650095.1	s__Clostridium_AD sp016650095	82.7731	610	1581	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium_AD	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002403785.1	s__Clostridium_AD sp002403785	81.6856	624	1581	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium_AD	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018861865.1	s__Clostridium_AD frigoris	81.018	684	1581	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium_AD	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:41:40,749] [INFO] GTDB search result was written to GCF_020176155.1_ASM2017615v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:41:40,749] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:41:40,753] [INFO] DFAST_QC result json was written to GCF_020176155.1_ASM2017615v1_genomic.fna/dqc_result.json
[2024-01-24 13:41:40,753] [INFO] DFAST_QC completed!
[2024-01-24 13:41:40,753] [INFO] Total running time: 0h1m7s