[2024-01-25 19:07:50,495] [INFO] DFAST_QC pipeline started.
[2024-01-25 19:07:50,496] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 19:07:50,496] [INFO] DQC Reference Directory: /var/lib/cwl/stgeb5a8114-548d-42fe-b576-c6df363d76c9/dqc_reference
[2024-01-25 19:07:51,706] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 19:07:51,707] [INFO] Task started: Prodigal
[2024-01-25 19:07:51,708] [INFO] Running command: gunzip -c /var/lib/cwl/stg5d7acf93-71df-45f0-80e2-d83521762d70/GCF_020614375.1_ASM2061437v1_genomic.fna.gz | prodigal -d GCF_020614375.1_ASM2061437v1_genomic.fna/cds.fna -a GCF_020614375.1_ASM2061437v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 19:08:12,502] [INFO] Task succeeded: Prodigal
[2024-01-25 19:08:12,503] [INFO] Task started: HMMsearch
[2024-01-25 19:08:12,503] [INFO] Running command: hmmsearch --tblout GCF_020614375.1_ASM2061437v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgeb5a8114-548d-42fe-b576-c6df363d76c9/dqc_reference/reference_markers.hmm GCF_020614375.1_ASM2061437v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 19:08:12,781] [INFO] Task succeeded: HMMsearch
[2024-01-25 19:08:12,782] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg5d7acf93-71df-45f0-80e2-d83521762d70/GCF_020614375.1_ASM2061437v1_genomic.fna.gz]
[2024-01-25 19:08:12,822] [INFO] Query marker FASTA was written to GCF_020614375.1_ASM2061437v1_genomic.fna/markers.fasta
[2024-01-25 19:08:12,823] [INFO] Task started: Blastn
[2024-01-25 19:08:12,823] [INFO] Running command: blastn -query GCF_020614375.1_ASM2061437v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgeb5a8114-548d-42fe-b576-c6df363d76c9/dqc_reference/reference_markers.fasta -out GCF_020614375.1_ASM2061437v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:08:13,314] [INFO] Task succeeded: Blastn
[2024-01-25 19:08:13,316] [INFO] Selected 7 target genomes.
[2024-01-25 19:08:13,317] [INFO] Target genome list was writen to GCF_020614375.1_ASM2061437v1_genomic.fna/target_genomes.txt
[2024-01-25 19:08:13,323] [INFO] Task started: fastANI
[2024-01-25 19:08:13,323] [INFO] Running command: fastANI --query /var/lib/cwl/stg5d7acf93-71df-45f0-80e2-d83521762d70/GCF_020614375.1_ASM2061437v1_genomic.fna.gz --refList GCF_020614375.1_ASM2061437v1_genomic.fna/target_genomes.txt --output GCF_020614375.1_ASM2061437v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 19:08:20,570] [INFO] Task succeeded: fastANI
[2024-01-25 19:08:20,570] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgeb5a8114-548d-42fe-b576-c6df363d76c9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 19:08:20,570] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgeb5a8114-548d-42fe-b576-c6df363d76c9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 19:08:20,576] [INFO] Found 7 fastANI hits (0 hits with ANI > threshold)
[2024-01-25 19:08:20,577] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-25 19:08:20,577] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Haladaptatus paucihalophilus	strain=DX253	GCA_900142335.1	367189	367189	type	True	82.0341	795	1673	95	below_threshold
Haladaptatus paucihalophilus	strain=DX253	GCA_000376445.1	367189	367189	type	True	82.0166	798	1673	95	below_threshold
Haladaptatus paucihalophilus	strain=DX253	GCA_000187225.2	367189	367189	type	True	81.9985	799	1673	95	below_threshold
Haladaptatus pallidirubidus	strain=YIM 93656	GCA_020700235.1	1008152	1008152	type	True	81.5135	946	1673	95	below_threshold
Haladaptatus cibarius	strain=D43	GCA_000710615.1	453847	453847	type	True	81.0977	731	1673	95	below_threshold
Haladaptatus litoreus	strain=CGMCC 1.7737	GCA_900156425.1	553468	553468	type	True	81.0829	841	1673	95	below_threshold
Haloferax gibbonsii	strain=ATCC 33959	GCA_000336775.1	35746	35746	type	True	78.1987	470	1673	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 19:08:20,578] [INFO] DFAST Taxonomy check result was written to GCF_020614375.1_ASM2061437v1_genomic.fna/tc_result.tsv
[2024-01-25 19:08:20,579] [INFO] ===== Taxonomy check completed =====
[2024-01-25 19:08:20,579] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 19:08:20,579] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgeb5a8114-548d-42fe-b576-c6df363d76c9/dqc_reference/checkm_data
[2024-01-25 19:08:20,580] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 19:08:20,630] [INFO] Task started: CheckM
[2024-01-25 19:08:20,630] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_020614375.1_ASM2061437v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_020614375.1_ASM2061437v1_genomic.fna/checkm_input GCF_020614375.1_ASM2061437v1_genomic.fna/checkm_result
[2024-01-25 19:09:15,244] [INFO] Task succeeded: CheckM
[2024-01-25 19:09:15,245] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 19:09:15,263] [INFO] ===== Completeness check finished =====
[2024-01-25 19:09:15,263] [INFO] ===== Start GTDB Search =====
[2024-01-25 19:09:15,263] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_020614375.1_ASM2061437v1_genomic.fna/markers.fasta)
[2024-01-25 19:09:15,264] [INFO] Task started: Blastn
[2024-01-25 19:09:15,264] [INFO] Running command: blastn -query GCF_020614375.1_ASM2061437v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgeb5a8114-548d-42fe-b576-c6df363d76c9/dqc_reference/reference_markers_gtdb.fasta -out GCF_020614375.1_ASM2061437v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:09:15,776] [INFO] Task succeeded: Blastn
[2024-01-25 19:09:15,779] [INFO] Selected 8 target genomes.
[2024-01-25 19:09:15,779] [INFO] Target genome list was writen to GCF_020614375.1_ASM2061437v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 19:09:15,807] [INFO] Task started: fastANI
[2024-01-25 19:09:15,807] [INFO] Running command: fastANI --query /var/lib/cwl/stg5d7acf93-71df-45f0-80e2-d83521762d70/GCF_020614375.1_ASM2061437v1_genomic.fna.gz --refList GCF_020614375.1_ASM2061437v1_genomic.fna/target_genomes_gtdb.txt --output GCF_020614375.1_ASM2061437v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 19:09:23,628] [INFO] Task succeeded: fastANI
[2024-01-25 19:09:23,634] [INFO] Found 8 fastANI hits (0 hits with ANI > circumscription radius)
[2024-01-25 19:09:23,635] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000376445.1	s__Haladaptatus paucihalophilus	82.0299	794	1673	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Haladaptatus	95.0	98.84	96.52	0.92	0.76	4	-
GCF_000710615.1	s__Haladaptatus cibarius	81.1098	730	1673	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Haladaptatus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900156425.1	s__Haladaptatus litoreus	81.0906	841	1673	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Haladaptatus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001625445.1	s__Haladaptatus sp001625445	80.7559	801	1673	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Haladaptatus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003298465.1	s__Haladaptatus sp003298465	80.6861	732	1673	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Haladaptatus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000336775.1	s__Haloferax gibbonsii	78.1954	471	1673	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Haloferax	96.8304	98.89	98.78	0.89	0.88	5	-
GCF_000336815.1	s__Haloferax prahovense	78.0986	465	1673	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Haloferax	96.8304	99.29	99.22	0.91	0.91	5	-
GCF_900215575.1	s__Natronoarchaeum philippinense	77.6776	320	1673	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natronoarchaeaceae;g__Natronoarchaeum	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 19:09:23,638] [INFO] GTDB search result was written to GCF_020614375.1_ASM2061437v1_genomic.fna/result_gtdb.tsv
[2024-01-25 19:09:23,638] [INFO] ===== GTDB Search completed =====
[2024-01-25 19:09:23,641] [INFO] DFAST_QC result json was written to GCF_020614375.1_ASM2061437v1_genomic.fna/dqc_result.json
[2024-01-25 19:09:23,641] [INFO] DFAST_QC completed!
[2024-01-25 19:09:23,641] [INFO] Total running time: 0h1m33s