[2024-01-24 12:36:24,680] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:36:24,685] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:36:24,686] [INFO] DQC Reference Directory: /var/lib/cwl/stg306c96d0-6164-41a4-9df7-323c26831d06/dqc_reference
[2024-01-24 12:36:26,097] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:36:26,098] [INFO] Task started: Prodigal
[2024-01-24 12:36:26,098] [INFO] Running command: gunzip -c /var/lib/cwl/stg28bf754b-8291-4c60-8119-b180c47a1b91/GCF_020735785.1_ASM2073578v1_genomic.fna.gz | prodigal -d GCF_020735785.1_ASM2073578v1_genomic.fna/cds.fna -a GCF_020735785.1_ASM2073578v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:36:40,089] [INFO] Task succeeded: Prodigal
[2024-01-24 12:36:40,090] [INFO] Task started: HMMsearch
[2024-01-24 12:36:40,090] [INFO] Running command: hmmsearch --tblout GCF_020735785.1_ASM2073578v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg306c96d0-6164-41a4-9df7-323c26831d06/dqc_reference/reference_markers.hmm GCF_020735785.1_ASM2073578v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:36:40,352] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:36:40,353] [INFO] Found 6/6 markers.
[2024-01-24 12:36:40,379] [INFO] Query marker FASTA was written to GCF_020735785.1_ASM2073578v1_genomic.fna/markers.fasta
[2024-01-24 12:36:40,379] [INFO] Task started: Blastn
[2024-01-24 12:36:40,380] [INFO] Running command: blastn -query GCF_020735785.1_ASM2073578v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg306c96d0-6164-41a4-9df7-323c26831d06/dqc_reference/reference_markers.fasta -out GCF_020735785.1_ASM2073578v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:36:40,996] [INFO] Task succeeded: Blastn
[2024-01-24 12:36:40,999] [INFO] Selected 9 target genomes.
[2024-01-24 12:36:41,000] [INFO] Target genome list was writen to GCF_020735785.1_ASM2073578v1_genomic.fna/target_genomes.txt
[2024-01-24 12:36:41,007] [INFO] Task started: fastANI
[2024-01-24 12:36:41,008] [INFO] Running command: fastANI --query /var/lib/cwl/stg28bf754b-8291-4c60-8119-b180c47a1b91/GCF_020735785.1_ASM2073578v1_genomic.fna.gz --refList GCF_020735785.1_ASM2073578v1_genomic.fna/target_genomes.txt --output GCF_020735785.1_ASM2073578v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:36:49,258] [INFO] Task succeeded: fastANI
[2024-01-24 12:36:49,258] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg306c96d0-6164-41a4-9df7-323c26831d06/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:36:49,259] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg306c96d0-6164-41a4-9df7-323c26831d06/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:36:49,278] [INFO] Found 9 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 12:36:49,279] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:36:49,279] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Prevotella melaninogenica	strain=FDAARGOS_1566	GCA_020735785.1	28132	28132	suspected-type	True	100.0	1054	1055	95	conclusive
Prevotella melaninogenica	strain=ATCC 25845	GCA_000144405.1	28132	28132	suspected-type	True	99.9992	1053	1055	95	conclusive
Prevotella scopos	strain=DSM 22613	GCA_900129535.1	589437	589437	type	True	88.2279	787	1055	95	below_threshold
Prevotella scopos	strain=JCM 17725	GCA_000614285.1	589437	589437	type	True	88.2107	768	1055	95	below_threshold
Prevotella scopos	strain=W2052	GCA_018127785.1	589437	589437	type	True	88.1174	814	1055	95	below_threshold
Prevotella scopos	strain=W2052	GCA_001683355.1	589437	589437	type	True	88.101	818	1055	95	below_threshold
Prevotella jejuni	strain=DSM 26989	GCA_900187995.1	1177574	1177574	type	True	86.7692	779	1055	95	below_threshold
Prevotella vespertina	strain=A2879	GCA_009728485.1	2608404	2608404	type	True	81.1166	405	1055	95	below_threshold
Hallella faecis	strain=CLA-AA-H145	GCA_018789675.1	2841596	2841596	type	True	77.3144	56	1055	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:36:49,282] [INFO] DFAST Taxonomy check result was written to GCF_020735785.1_ASM2073578v1_genomic.fna/tc_result.tsv
[2024-01-24 12:36:49,282] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:36:49,283] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:36:49,283] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg306c96d0-6164-41a4-9df7-323c26831d06/dqc_reference/checkm_data
[2024-01-24 12:36:49,284] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:36:49,316] [INFO] Task started: CheckM
[2024-01-24 12:36:49,316] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_020735785.1_ASM2073578v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_020735785.1_ASM2073578v1_genomic.fna/checkm_input GCF_020735785.1_ASM2073578v1_genomic.fna/checkm_result
[2024-01-24 12:37:33,117] [INFO] Task succeeded: CheckM
[2024-01-24 12:37:33,118] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:37:33,134] [INFO] ===== Completeness check finished =====
[2024-01-24 12:37:33,135] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:37:33,135] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_020735785.1_ASM2073578v1_genomic.fna/markers.fasta)
[2024-01-24 12:37:33,136] [INFO] Task started: Blastn
[2024-01-24 12:37:33,136] [INFO] Running command: blastn -query GCF_020735785.1_ASM2073578v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg306c96d0-6164-41a4-9df7-323c26831d06/dqc_reference/reference_markers_gtdb.fasta -out GCF_020735785.1_ASM2073578v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:37:33,922] [INFO] Task succeeded: Blastn
[2024-01-24 12:37:33,926] [INFO] Selected 8 target genomes.
[2024-01-24 12:37:33,926] [INFO] Target genome list was writen to GCF_020735785.1_ASM2073578v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:37:33,934] [INFO] Task started: fastANI
[2024-01-24 12:37:33,935] [INFO] Running command: fastANI --query /var/lib/cwl/stg28bf754b-8291-4c60-8119-b180c47a1b91/GCF_020735785.1_ASM2073578v1_genomic.fna.gz --refList GCF_020735785.1_ASM2073578v1_genomic.fna/target_genomes_gtdb.txt --output GCF_020735785.1_ASM2073578v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:37:42,334] [INFO] Task succeeded: fastANI
[2024-01-24 12:37:42,345] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:37:42,345] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000144405.1	s__Prevotella melaninogenica	99.9992	1053	1055	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	96.46	95.36	0.87	0.78	36	conclusive
GCF_018127965.1	s__Prevotella melaninogenica_B	94.8856	875	1055	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	95.77	95.72	0.91	0.89	4	-
GCF_018127925.1	s__Prevotella melaninogenica_C	94.7517	883	1055	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003609775.1	s__Prevotella melaninogenica_A	90.9301	820	1055	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018127785.1	s__Prevotella scopos	88.1127	813	1055	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	99.98	99.97	1.00	1.00	4	-
GCF_018128065.1	s__Prevotella sp000467895	87.9382	777	1055	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	97.71	96.42	0.91	0.83	4	-
GCF_900187995.1	s__Prevotella jejuni	86.7442	782	1055	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	97.22	96.57	0.92	0.88	7	-
GCF_000257925.1	s__Prevotella sp000257925	81.7852	427	1055	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	97.40	96.99	0.92	0.83	10	-
--------------------------------------------------------------------------------
[2024-01-24 12:37:42,347] [INFO] GTDB search result was written to GCF_020735785.1_ASM2073578v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:37:42,348] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:37:42,351] [INFO] DFAST_QC result json was written to GCF_020735785.1_ASM2073578v1_genomic.fna/dqc_result.json
[2024-01-24 12:37:42,351] [INFO] DFAST_QC completed!
[2024-01-24 12:37:42,351] [INFO] Total running time: 0h1m18s