[2024-01-24 13:28:25,979] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:28:25,981] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:28:25,981] [INFO] DQC Reference Directory: /var/lib/cwl/stge5a8bd9d-50f7-461d-b754-7e1475e71878/dqc_reference
[2024-01-24 13:28:27,236] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:28:27,237] [INFO] Task started: Prodigal
[2024-01-24 13:28:27,237] [INFO] Running command: gunzip -c /var/lib/cwl/stgac8a512a-ba01-479d-989a-e697220e1770/GCF_020858175.1_ASM2085817v1_genomic.fna.gz | prodigal -d GCF_020858175.1_ASM2085817v1_genomic.fna/cds.fna -a GCF_020858175.1_ASM2085817v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:28:51,277] [INFO] Task succeeded: Prodigal
[2024-01-24 13:28:51,277] [INFO] Task started: HMMsearch
[2024-01-24 13:28:51,277] [INFO] Running command: hmmsearch --tblout GCF_020858175.1_ASM2085817v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stge5a8bd9d-50f7-461d-b754-7e1475e71878/dqc_reference/reference_markers.hmm GCF_020858175.1_ASM2085817v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:28:51,629] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:28:51,630] [INFO] Found 6/6 markers.
[2024-01-24 13:28:51,693] [INFO] Query marker FASTA was written to GCF_020858175.1_ASM2085817v1_genomic.fna/markers.fasta
[2024-01-24 13:28:51,693] [INFO] Task started: Blastn
[2024-01-24 13:28:51,693] [INFO] Running command: blastn -query GCF_020858175.1_ASM2085817v1_genomic.fna/markers.fasta -db /var/lib/cwl/stge5a8bd9d-50f7-461d-b754-7e1475e71878/dqc_reference/reference_markers.fasta -out GCF_020858175.1_ASM2085817v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:28:52,686] [INFO] Task succeeded: Blastn
[2024-01-24 13:28:52,689] [INFO] Selected 10 target genomes.
[2024-01-24 13:28:52,690] [INFO] Target genome list was writen to GCF_020858175.1_ASM2085817v1_genomic.fna/target_genomes.txt
[2024-01-24 13:28:52,706] [INFO] Task started: fastANI
[2024-01-24 13:28:52,706] [INFO] Running command: fastANI --query /var/lib/cwl/stgac8a512a-ba01-479d-989a-e697220e1770/GCF_020858175.1_ASM2085817v1_genomic.fna.gz --refList GCF_020858175.1_ASM2085817v1_genomic.fna/target_genomes.txt --output GCF_020858175.1_ASM2085817v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:29:09,861] [INFO] Task succeeded: fastANI
[2024-01-24 13:29:09,861] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stge5a8bd9d-50f7-461d-b754-7e1475e71878/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:29:09,862] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stge5a8bd9d-50f7-461d-b754-7e1475e71878/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:29:09,871] [INFO] Found 10 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 13:29:09,871] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:29:09,871] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Janthinobacterium lividum	strain=ATCC 12473	GCA_020858175.1	29581	29581	suspected-type	True	100.0	2212	2215	95	conclusive
Janthinobacterium lividum	strain=NCTC9796	GCA_900451145.1	29581	29581	suspected-type	True	99.9979	2213	2215	95	conclusive
Janthinobacterium lividum	strain=H-24	GCA_001758635.1	29581	29581	suspected-type	True	99.9698	2157	2215	95	conclusive
Janthinobacterium violaceinigrum	strain=FT13W	GCA_009208555.1	2654252	2654252	type	True	92.6918	1815	2215	95	below_threshold
Janthinobacterium tructae	strain=SNU WT3	GCA_006517255.1	2590869	2590869	type	True	92.609	1769	2215	95	below_threshold
Rugamonas fusca	strain=FT3S	GCA_014042365.1	2758568	2758568	type	True	81.3828	1012	2215	95	below_threshold
Rugamonas brunnea	strain=LX20W	GCA_014042345.1	2758569	2758569	type	True	81.2944	1001	2215	95	below_threshold
Rugamonas apoptosis	strain=LX47W	GCA_014042355.1	2758570	2758570	type	True	81.2823	983	2215	95	below_threshold
Duganella aceris	strain=SAP-35	GCA_011090165.1	2703883	2703883	type	True	80.6553	1031	2215	95	below_threshold
Massilia cavernae	strain=K1S02-61	GCA_003590855.1	2320864	2320864	type	True	80.0391	708	2215	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:29:09,873] [INFO] DFAST Taxonomy check result was written to GCF_020858175.1_ASM2085817v1_genomic.fna/tc_result.tsv
[2024-01-24 13:29:09,873] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:29:09,873] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:29:09,874] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stge5a8bd9d-50f7-461d-b754-7e1475e71878/dqc_reference/checkm_data
[2024-01-24 13:29:09,875] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:29:09,937] [INFO] Task started: CheckM
[2024-01-24 13:29:09,938] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_020858175.1_ASM2085817v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_020858175.1_ASM2085817v1_genomic.fna/checkm_input GCF_020858175.1_ASM2085817v1_genomic.fna/checkm_result
[2024-01-24 13:30:14,279] [INFO] Task succeeded: CheckM
[2024-01-24 13:30:14,280] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:30:14,301] [INFO] ===== Completeness check finished =====
[2024-01-24 13:30:14,301] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:30:14,301] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_020858175.1_ASM2085817v1_genomic.fna/markers.fasta)
[2024-01-24 13:30:14,302] [INFO] Task started: Blastn
[2024-01-24 13:30:14,302] [INFO] Running command: blastn -query GCF_020858175.1_ASM2085817v1_genomic.fna/markers.fasta -db /var/lib/cwl/stge5a8bd9d-50f7-461d-b754-7e1475e71878/dqc_reference/reference_markers_gtdb.fasta -out GCF_020858175.1_ASM2085817v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:30:15,941] [INFO] Task succeeded: Blastn
[2024-01-24 13:30:15,945] [INFO] Selected 13 target genomes.
[2024-01-24 13:30:15,945] [INFO] Target genome list was writen to GCF_020858175.1_ASM2085817v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:30:15,959] [INFO] Task started: fastANI
[2024-01-24 13:30:15,959] [INFO] Running command: fastANI --query /var/lib/cwl/stgac8a512a-ba01-479d-989a-e697220e1770/GCF_020858175.1_ASM2085817v1_genomic.fna.gz --refList GCF_020858175.1_ASM2085817v1_genomic.fna/target_genomes_gtdb.txt --output GCF_020858175.1_ASM2085817v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:30:37,943] [INFO] Task succeeded: fastANI
[2024-01-24 13:30:37,961] [INFO] Found 13 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:30:37,961] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_900451145.1	s__Janthinobacterium lividum	99.9979	2213	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0	97.96	97.56	0.93	0.89	12	conclusive
GCF_002002885.1	s__Janthinobacterium sp002002885	93.9058	1846	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0	96.09	96.09	0.93	0.93	2	-
GCF_002735705.1	s__Janthinobacterium sp002735705	93.8559	1850	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000745325.1	s__Janthinobacterium sp000745325	93.5551	1847	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0	97.59	97.59	0.95	0.94	3	-
GCF_000632025.1	s__Janthinobacterium lividum_C	93.4133	1856	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0	97.00	96.24	0.94	0.92	5	-
GCF_009208735.1	s__Janthinobacterium sp009208735	93.3876	1819	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0	95.70	95.70	0.90	0.90	2	-
GCF_011601275.1	s__Janthinobacterium lividum_E	93.2009	1840	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0	96.41	96.40	0.93	0.92	4	-
GCF_003667705.1	s__Janthinobacterium sp001758725	93.1163	1800	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0774	98.10	97.46	0.93	0.89	7	-
GCF_002127655.1	s__Janthinobacterium sp002127655	92.737	1786	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0774	99.99	99.99	0.99	0.99	4	-
GCF_009208555.1	s__Janthinobacterium violaceinigrum	92.6897	1815	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0	96.88	96.88	0.91	0.91	2	-
GCF_900156175.1	s__Janthinobacterium sp900156175	91.8777	1744	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900112025.1	s__Janthinobacterium sp900112025	91.2792	1791	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0	99.99	99.99	0.99	0.99	2	-
GCA_009923995.1	s__Janthinobacterium sp009923995	91.1818	1703	2215	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Janthinobacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:30:37,962] [INFO] GTDB search result was written to GCF_020858175.1_ASM2085817v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:30:37,963] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:30:37,966] [INFO] DFAST_QC result json was written to GCF_020858175.1_ASM2085817v1_genomic.fna/dqc_result.json
[2024-01-24 13:30:37,966] [INFO] DFAST_QC completed!
[2024-01-24 13:30:37,966] [INFO] Total running time: 0h2m12s