[2024-01-25 20:21:05,568] [INFO] DFAST_QC pipeline started.
[2024-01-25 20:21:05,570] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 20:21:05,570] [INFO] DQC Reference Directory: /var/lib/cwl/stg5c97ada5-7ac0-48be-9705-c7decf3c757a/dqc_reference
[2024-01-25 20:21:06,705] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 20:21:06,706] [INFO] Task started: Prodigal
[2024-01-25 20:21:06,706] [INFO] Running command: gunzip -c /var/lib/cwl/stg8b25901e-fc08-47f4-b64b-e17b8eb462ff/GCF_021018765.1_ASM2101876v1_genomic.fna.gz | prodigal -d GCF_021018765.1_ASM2101876v1_genomic.fna/cds.fna -a GCF_021018765.1_ASM2101876v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 20:21:29,183] [INFO] Task succeeded: Prodigal
[2024-01-25 20:21:29,183] [INFO] Task started: HMMsearch
[2024-01-25 20:21:29,183] [INFO] Running command: hmmsearch --tblout GCF_021018765.1_ASM2101876v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg5c97ada5-7ac0-48be-9705-c7decf3c757a/dqc_reference/reference_markers.hmm GCF_021018765.1_ASM2101876v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 20:21:29,463] [INFO] Task succeeded: HMMsearch
[2024-01-25 20:21:29,465] [INFO] Found 6/6 markers.
[2024-01-25 20:21:29,516] [INFO] Query marker FASTA was written to GCF_021018765.1_ASM2101876v1_genomic.fna/markers.fasta
[2024-01-25 20:21:29,516] [INFO] Task started: Blastn
[2024-01-25 20:21:29,516] [INFO] Running command: blastn -query GCF_021018765.1_ASM2101876v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg5c97ada5-7ac0-48be-9705-c7decf3c757a/dqc_reference/reference_markers.fasta -out GCF_021018765.1_ASM2101876v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:21:30,336] [INFO] Task succeeded: Blastn
[2024-01-25 20:21:30,340] [INFO] Selected 19 target genomes.
[2024-01-25 20:21:30,341] [INFO] Target genome list was writen to GCF_021018765.1_ASM2101876v1_genomic.fna/target_genomes.txt
[2024-01-25 20:21:30,346] [INFO] Task started: fastANI
[2024-01-25 20:21:30,346] [INFO] Running command: fastANI --query /var/lib/cwl/stg8b25901e-fc08-47f4-b64b-e17b8eb462ff/GCF_021018765.1_ASM2101876v1_genomic.fna.gz --refList GCF_021018765.1_ASM2101876v1_genomic.fna/target_genomes.txt --output GCF_021018765.1_ASM2101876v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 20:21:57,293] [INFO] Task succeeded: fastANI
[2024-01-25 20:21:57,294] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg5c97ada5-7ac0-48be-9705-c7decf3c757a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 20:21:57,294] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg5c97ada5-7ac0-48be-9705-c7decf3c757a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 20:21:57,304] [INFO] Found 16 fastANI hits (0 hits with ANI > threshold)
[2024-01-25 20:21:57,304] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-25 20:21:57,305] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Pseudomonas pharyngis	strain=BML-PP036	GCA_021602345.1	2892333	2892333	type	True	94.1662	1798	2164	95	below_threshold
Pseudomonas sputi	strain=BML-PP014	GCA_021603585.1	2892325	2892325	type	True	93.7797	1773	2164	95	below_threshold
Pseudomonas allokribbensis	strain=IzPS23	GCA_014863605.1	2774460	2774460	type	True	92.0693	1804	2164	95	below_threshold
Pseudomonas kribbensis	strain=46-2	GCA_003352185.1	1628086	1628086	type	True	91.8936	1805	2164	95	below_threshold
Pseudomonas bananamidigenes	strain=BW11P2	GCA_001679645.1	2843610	2843610	type	True	90.5235	1700	2164	95	below_threshold
Pseudomonas koreensis	strain=DSM 16610	GCA_012986595.1	198620	198620	suspected-type	True	88.9771	1660	2164	95	below_threshold
Pseudomonas koreensis	strain=JCM 14769	GCA_014646955.1	198620	198620	suspected-type	True	88.9364	1661	2164	95	below_threshold
Pseudomonas monsensis	strain=PGSB 8459	GCA_014268495.2	2745509	2745509	type	True	88.5699	1693	2164	95	below_threshold
Pseudomonas germanica	strain=FIT28	GCA_019614655.1	2815720	2815720	type	True	88.4668	1781	2164	95	below_threshold
Pseudomonas crudilactis	strain=UCMA 17988	GCA_013433315.1	2697028	2697028	type	True	88.1532	1715	2164	95	below_threshold
Pseudomonas granadensis	strain=LMG 27940	GCA_900105485.1	1421430	1421430	suspected-type	True	87.5391	1563	2164	95	below_threshold
Pseudomonas moorei	strain=CCUG 53114	GCA_008801475.1	395599	395599	type	True	86.1236	1386	2164	95	below_threshold
Pseudomonas rhizophila	strain=S211	GCA_003033885.1	2045200	2045200	type	True	83.9938	1328	2164	95	below_threshold
Pseudomonas pergaminensis	strain=1008	GCA_024112395.1	2853159	2853159	type	True	83.9362	1323	2164	95	below_threshold
Streptomyces pactum	strain=ATCC 27456	GCA_016031615.1	68249	68249	type	True	75.1873	94	2164	95	below_threshold
Streptomyces adustus	strain=NBRC 109810	GCA_009377175.1	1609272	1609272	type	True	74.9239	88	2164	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 20:21:57,306] [INFO] DFAST Taxonomy check result was written to GCF_021018765.1_ASM2101876v1_genomic.fna/tc_result.tsv
[2024-01-25 20:21:57,306] [INFO] ===== Taxonomy check completed =====
[2024-01-25 20:21:57,307] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 20:21:57,307] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg5c97ada5-7ac0-48be-9705-c7decf3c757a/dqc_reference/checkm_data
[2024-01-25 20:21:57,308] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 20:21:57,365] [INFO] Task started: CheckM
[2024-01-25 20:21:57,365] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_021018765.1_ASM2101876v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_021018765.1_ASM2101876v1_genomic.fna/checkm_input GCF_021018765.1_ASM2101876v1_genomic.fna/checkm_result
[2024-01-25 20:23:14,336] [INFO] Task succeeded: CheckM
[2024-01-25 20:23:14,337] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 20:23:14,361] [INFO] ===== Completeness check finished =====
[2024-01-25 20:23:14,362] [INFO] ===== Start GTDB Search =====
[2024-01-25 20:23:14,363] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_021018765.1_ASM2101876v1_genomic.fna/markers.fasta)
[2024-01-25 20:23:14,363] [INFO] Task started: Blastn
[2024-01-25 20:23:14,363] [INFO] Running command: blastn -query GCF_021018765.1_ASM2101876v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg5c97ada5-7ac0-48be-9705-c7decf3c757a/dqc_reference/reference_markers_gtdb.fasta -out GCF_021018765.1_ASM2101876v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:23:15,796] [INFO] Task succeeded: Blastn
[2024-01-25 20:23:15,799] [INFO] Selected 14 target genomes.
[2024-01-25 20:23:15,799] [INFO] Target genome list was writen to GCF_021018765.1_ASM2101876v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 20:23:15,810] [INFO] Task started: fastANI
[2024-01-25 20:23:15,810] [INFO] Running command: fastANI --query /var/lib/cwl/stg8b25901e-fc08-47f4-b64b-e17b8eb462ff/GCF_021018765.1_ASM2101876v1_genomic.fna.gz --refList GCF_021018765.1_ASM2101876v1_genomic.fna/target_genomes_gtdb.txt --output GCF_021018765.1_ASM2101876v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 20:23:39,376] [INFO] Task succeeded: fastANI
[2024-01-25 20:23:39,386] [INFO] Found 14 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 20:23:39,386] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_004006335.1	s__Pseudomonas_E sp002112885	95.1834	1865	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	96.01	95.48	0.92	0.90	14	conclusive
GCF_003352875.1	s__Pseudomonas_E fluorescens_BF	94.8554	1876	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014863585.1	s__Pseudomonas_E gozinkensis	94.4907	1872	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_010448615.1	s__Pseudomonas_E sp001655595	94.3964	1848	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	97.61	96.24	0.94	0.90	22	-
GCF_001594225.2	s__Pseudomonas_E glycinae	94.1872	1813	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	97.56	95.98	0.93	0.88	9	-
GCF_002113045.1	s__Pseudomonas_E sp002113045	93.9007	1781	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	98.93	98.93	0.93	0.93	2	-
GCF_012974785.1	s__Pseudomonas_E fluorescens_BT	92.3311	1821	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	98.87	98.87	0.96	0.96	2	-
GCF_014863605.1	s__Pseudomonas_E allokribbensis	92.0629	1803	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	97.79	97.76	0.93	0.92	10	-
GCF_003352185.1	s__Pseudomonas_E kribbensis	91.8882	1807	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	96.22	95.68	0.92	0.89	10	-
GCF_003999415.1	s__Pseudomonas_E koreensis_E	88.9688	1660	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	98.57	98.57	0.92	0.92	2	-
GCF_014472415.1	s__Pseudomonas_E sp014472415	88.2988	1621	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900580865.1	s__Pseudomonas_E sp900580865	88.2204	1728	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009834515.1	s__Pseudomonas_E sp009834515	88.1437	1709	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900636825.1	s__Pseudomonas_E fluorescens_BG	87.0837	1544	2164	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 20:23:39,387] [INFO] GTDB search result was written to GCF_021018765.1_ASM2101876v1_genomic.fna/result_gtdb.tsv
[2024-01-25 20:23:39,388] [INFO] ===== GTDB Search completed =====
[2024-01-25 20:23:39,391] [INFO] DFAST_QC result json was written to GCF_021018765.1_ASM2101876v1_genomic.fna/dqc_result.json
[2024-01-25 20:23:39,392] [INFO] DFAST_QC completed!
[2024-01-25 20:23:39,392] [INFO] Total running time: 0h2m34s