[2024-01-24 13:28:10,113] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:28:10,115] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:28:10,115] [INFO] DQC Reference Directory: /var/lib/cwl/stgcd3cd927-751f-4f42-b2dc-0b251da3bc70/dqc_reference
[2024-01-24 13:28:11,287] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:28:11,288] [INFO] Task started: Prodigal
[2024-01-24 13:28:11,288] [INFO] Running command: gunzip -c /var/lib/cwl/stg72f03d8b-fd5a-40ae-bd5e-42d45131079d/GCF_021648885.1_ASM2164888v1_genomic.fna.gz | prodigal -d GCF_021648885.1_ASM2164888v1_genomic.fna/cds.fna -a GCF_021648885.1_ASM2164888v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:28:23,382] [INFO] Task succeeded: Prodigal
[2024-01-24 13:28:23,383] [INFO] Task started: HMMsearch
[2024-01-24 13:28:23,383] [INFO] Running command: hmmsearch --tblout GCF_021648885.1_ASM2164888v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgcd3cd927-751f-4f42-b2dc-0b251da3bc70/dqc_reference/reference_markers.hmm GCF_021648885.1_ASM2164888v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:28:23,705] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:28:23,707] [INFO] Found 6/6 markers.
[2024-01-24 13:28:23,758] [INFO] Query marker FASTA was written to GCF_021648885.1_ASM2164888v1_genomic.fna/markers.fasta
[2024-01-24 13:28:23,758] [INFO] Task started: Blastn
[2024-01-24 13:28:23,758] [INFO] Running command: blastn -query GCF_021648885.1_ASM2164888v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgcd3cd927-751f-4f42-b2dc-0b251da3bc70/dqc_reference/reference_markers.fasta -out GCF_021648885.1_ASM2164888v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:28:25,127] [INFO] Task succeeded: Blastn
[2024-01-24 13:28:25,137] [INFO] Selected 14 target genomes.
[2024-01-24 13:28:25,137] [INFO] Target genome list was writen to GCF_021648885.1_ASM2164888v1_genomic.fna/target_genomes.txt
[2024-01-24 13:28:25,169] [INFO] Task started: fastANI
[2024-01-24 13:28:25,169] [INFO] Running command: fastANI --query /var/lib/cwl/stg72f03d8b-fd5a-40ae-bd5e-42d45131079d/GCF_021648885.1_ASM2164888v1_genomic.fna.gz --refList GCF_021648885.1_ASM2164888v1_genomic.fna/target_genomes.txt --output GCF_021648885.1_ASM2164888v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:28:42,116] [INFO] Task succeeded: fastANI
[2024-01-24 13:28:42,117] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgcd3cd927-751f-4f42-b2dc-0b251da3bc70/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:28:42,117] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgcd3cd927-751f-4f42-b2dc-0b251da3bc70/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:28:42,133] [INFO] Found 14 fastANI hits (0 hits with ANI > threshold)
[2024-01-24 13:28:42,133] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-24 13:28:42,133] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Nocardioides baculatus	strain=G10	GCA_016735675.1	2801337	2801337	type	True	88.5752	1126	1419	95	below_threshold
Nocardioides glacieisoli	strain=HLT3-15	GCA_004137245.1	1168730	1168730	type	True	88.128	1097	1419	95	below_threshold
Nocardioides zhouii	strain=HLT2-9	GCA_004137345.1	1168729	1168729	type	True	86.3009	1080	1419	95	below_threshold
Nocardioides exalbidus	strain=DSM 22017	GCA_900105585.1	402596	402596	type	True	85.7806	1032	1419	95	below_threshold
Nocardioides cavernae	strain=KCTC 39551	GCA_014779675.1	1921566	1921566	type	True	85.5639	1033	1419	95	below_threshold
Nocardioides cavernae	strain=DSM 29950	GCA_016907475.1	1921566	1921566	type	True	85.5593	1025	1419	95	below_threshold
Nocardioides ganghwensis	strain=9920	GCA_014779655.1	252230	252230	type	True	85.527	987	1419	95	below_threshold
Nocardioides lacusdianchii	strain=JXJ CY 38	GCA_020102855.1	2783664	2783664	type	True	85.3848	979	1419	95	below_threshold
Nocardioides alpinus	strain=DSM 23325	GCA_002836395.1	748909	748909	type	True	85.3226	1034	1419	95	below_threshold
Nocardioides okcheonensis	strain=MMS20-HV4-12	GCA_020991065.1	2894081	2894081	type	True	85.3147	1046	1419	95	below_threshold
Nocardioides alpinus	strain=CGMCC 1.10697	GCA_900112035.1	748909	748909	type	True	85.3133	1035	1419	95	below_threshold
Nocardioides furvisabuli	strain=JCM 13813	GCA_021083185.1	375542	375542	type	True	85.2894	957	1419	95	below_threshold
Nocardioides eburneiflavus	strain=MMS17-SY213	GCA_004785795.1	2518372	2518372	type	True	85.2295	1012	1419	95	below_threshold
Nocardioides hwasunensis	strain=19197	GCA_014779665.1	397258	397258	type	True	85.2085	1011	1419	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:28:42,135] [INFO] DFAST Taxonomy check result was written to GCF_021648885.1_ASM2164888v1_genomic.fna/tc_result.tsv
[2024-01-24 13:28:42,136] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:28:42,137] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:28:42,137] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgcd3cd927-751f-4f42-b2dc-0b251da3bc70/dqc_reference/checkm_data
[2024-01-24 13:28:42,138] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:28:42,180] [INFO] Task started: CheckM
[2024-01-24 13:28:42,180] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_021648885.1_ASM2164888v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_021648885.1_ASM2164888v1_genomic.fna/checkm_input GCF_021648885.1_ASM2164888v1_genomic.fna/checkm_result
[2024-01-24 13:29:45,007] [INFO] Task succeeded: CheckM
[2024-01-24 13:29:45,009] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:29:45,034] [INFO] ===== Completeness check finished =====
[2024-01-24 13:29:45,035] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:29:45,035] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_021648885.1_ASM2164888v1_genomic.fna/markers.fasta)
[2024-01-24 13:29:45,036] [INFO] Task started: Blastn
[2024-01-24 13:29:45,036] [INFO] Running command: blastn -query GCF_021648885.1_ASM2164888v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgcd3cd927-751f-4f42-b2dc-0b251da3bc70/dqc_reference/reference_markers_gtdb.fasta -out GCF_021648885.1_ASM2164888v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:29:47,158] [INFO] Task succeeded: Blastn
[2024-01-24 13:29:47,162] [INFO] Selected 18 target genomes.
[2024-01-24 13:29:47,162] [INFO] Target genome list was writen to GCF_021648885.1_ASM2164888v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:29:47,194] [INFO] Task started: fastANI
[2024-01-24 13:29:47,194] [INFO] Running command: fastANI --query /var/lib/cwl/stg72f03d8b-fd5a-40ae-bd5e-42d45131079d/GCF_021648885.1_ASM2164888v1_genomic.fna.gz --refList GCF_021648885.1_ASM2164888v1_genomic.fna/target_genomes_gtdb.txt --output GCF_021648885.1_ASM2164888v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:30:06,988] [INFO] Task succeeded: fastANI
[2024-01-24 13:30:07,007] [INFO] Found 18 fastANI hits (0 hits with ANI > circumscription radius)
[2024-01-24 13:30:07,008] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_016735675.1	s__Nocardioides sp016735675	88.5846	1125	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004137245.1	s__Nocardioides glacieisoli	88.1101	1099	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004137345.1	s__Nocardioides sp004137345	86.3236	1078	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900105585.1	s__Nocardioides exalbidus	85.7594	1034	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001428125.1	s__Nocardioides sp001428125	85.6376	994	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016907475.1	s__Nocardioides cavernae	85.5402	1027	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	100.00	100.00	1.00	1.00	2	-
GCF_014779655.1	s__Nocardioides ganghwensis	85.5139	988	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	100.00	100.00	1.00	1.00	2	-
GCF_013410035.1	s__Nocardioides cavernae_A	85.4253	1022	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003633575.1	s__Nocardioides sp003633575	85.4249	1054	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018275325.1	s__Nocardioides sp018275325	85.3808	1042	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001425025.1	s__Nocardioides sp001425025	85.3547	1034	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCA_009699265.1	s__Nocardioides sp009699265	85.3428	954	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_017310035.1	s__Nocardioides sp017310035	85.3421	962	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900112035.1	s__Nocardioides alpinus	85.2989	1037	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	99.99	99.99	1.00	1.00	2	-
GCF_017872795.1	s__Nocardioides sp017872795	85.2538	1002	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004785795.1	s__Nocardioides eburneiflavus	85.2499	1011	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014779665.1	s__Nocardioides hwasunensis	85.2117	1011	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_017086465.1	s__Nocardioides sp014265965	85.0822	957	1419	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Propionibacteriales;f__Nocardioidaceae;g__Nocardioides	95.0	100.00	100.00	1.00	1.00	2	-
--------------------------------------------------------------------------------
[2024-01-24 13:30:07,010] [INFO] GTDB search result was written to GCF_021648885.1_ASM2164888v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:30:07,010] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:30:07,014] [INFO] DFAST_QC result json was written to GCF_021648885.1_ASM2164888v1_genomic.fna/dqc_result.json
[2024-01-24 13:30:07,015] [INFO] DFAST_QC completed!
[2024-01-24 13:30:07,015] [INFO] Total running time: 0h1m57s