[2024-01-24 13:55:40,435] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:55:40,437] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:55:40,437] [INFO] DQC Reference Directory: /var/lib/cwl/stgf1b01149-7364-4ccf-b262-388af1394d7c/dqc_reference
[2024-01-24 13:55:41,690] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:55:41,691] [INFO] Task started: Prodigal
[2024-01-24 13:55:41,691] [INFO] Running command: gunzip -c /var/lib/cwl/stgddf9a290-29c6-44c2-bf05-29b9ba77860c/GCF_021729145.1_ASM2172914v1_genomic.fna.gz | prodigal -d GCF_021729145.1_ASM2172914v1_genomic.fna/cds.fna -a GCF_021729145.1_ASM2172914v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:56:05,940] [INFO] Task succeeded: Prodigal
[2024-01-24 13:56:05,940] [INFO] Task started: HMMsearch
[2024-01-24 13:56:05,940] [INFO] Running command: hmmsearch --tblout GCF_021729145.1_ASM2172914v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgf1b01149-7364-4ccf-b262-388af1394d7c/dqc_reference/reference_markers.hmm GCF_021729145.1_ASM2172914v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:56:06,337] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:56:06,338] [INFO] Found 6/6 markers.
[2024-01-24 13:56:06,394] [INFO] Query marker FASTA was written to GCF_021729145.1_ASM2172914v1_genomic.fna/markers.fasta
[2024-01-24 13:56:06,395] [INFO] Task started: Blastn
[2024-01-24 13:56:06,395] [INFO] Running command: blastn -query GCF_021729145.1_ASM2172914v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf1b01149-7364-4ccf-b262-388af1394d7c/dqc_reference/reference_markers.fasta -out GCF_021729145.1_ASM2172914v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:56:06,945] [INFO] Task succeeded: Blastn
[2024-01-24 13:56:06,949] [INFO] Selected 22 target genomes.
[2024-01-24 13:56:06,950] [INFO] Target genome list was writen to GCF_021729145.1_ASM2172914v1_genomic.fna/target_genomes.txt
[2024-01-24 13:56:07,018] [INFO] Task started: fastANI
[2024-01-24 13:56:07,018] [INFO] Running command: fastANI --query /var/lib/cwl/stgddf9a290-29c6-44c2-bf05-29b9ba77860c/GCF_021729145.1_ASM2172914v1_genomic.fna.gz --refList GCF_021729145.1_ASM2172914v1_genomic.fna/target_genomes.txt --output GCF_021729145.1_ASM2172914v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:56:21,393] [INFO] Task succeeded: fastANI
[2024-01-24 13:56:21,394] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgf1b01149-7364-4ccf-b262-388af1394d7c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:56:21,394] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgf1b01149-7364-4ccf-b262-388af1394d7c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:56:21,400] [INFO] Found 1 fastANI hits (0 hits with ANI > threshold)
[2024-01-24 13:56:21,400] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-24 13:56:21,400] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Membranihabitans marinus	strain=CZ-AZ5	GCA_021729155.1	1227546	1227546	type	True	76.4296	116	1946	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:56:21,402] [INFO] DFAST Taxonomy check result was written to GCF_021729145.1_ASM2172914v1_genomic.fna/tc_result.tsv
[2024-01-24 13:56:21,402] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:56:21,403] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:56:21,403] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgf1b01149-7364-4ccf-b262-388af1394d7c/dqc_reference/checkm_data
[2024-01-24 13:56:21,404] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:56:21,458] [INFO] Task started: CheckM
[2024-01-24 13:56:21,458] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_021729145.1_ASM2172914v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_021729145.1_ASM2172914v1_genomic.fna/checkm_input GCF_021729145.1_ASM2172914v1_genomic.fna/checkm_result
[2024-01-24 13:57:31,153] [INFO] Task succeeded: CheckM
[2024-01-24 13:57:31,154] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:57:31,177] [INFO] ===== Completeness check finished =====
[2024-01-24 13:57:31,177] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:57:31,178] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_021729145.1_ASM2172914v1_genomic.fna/markers.fasta)
[2024-01-24 13:57:31,178] [INFO] Task started: Blastn
[2024-01-24 13:57:31,178] [INFO] Running command: blastn -query GCF_021729145.1_ASM2172914v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf1b01149-7364-4ccf-b262-388af1394d7c/dqc_reference/reference_markers_gtdb.fasta -out GCF_021729145.1_ASM2172914v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:57:32,038] [INFO] Task succeeded: Blastn
[2024-01-24 13:57:32,042] [INFO] Selected 30 target genomes.
[2024-01-24 13:57:32,042] [INFO] Target genome list was writen to GCF_021729145.1_ASM2172914v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:57:32,081] [INFO] Task started: fastANI
[2024-01-24 13:57:32,081] [INFO] Running command: fastANI --query /var/lib/cwl/stgddf9a290-29c6-44c2-bf05-29b9ba77860c/GCF_021729145.1_ASM2172914v1_genomic.fna.gz --refList GCF_021729145.1_ASM2172914v1_genomic.fna/target_genomes_gtdb.txt --output GCF_021729145.1_ASM2172914v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:57:52,718] [INFO] Task succeeded: fastANI
[2024-01-24 13:57:52,720] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2024-01-24 13:57:52,720] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2024-01-24 13:57:52,783] [INFO] GTDB search result was written to GCF_021729145.1_ASM2172914v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:57:52,784] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:57:52,786] [INFO] DFAST_QC result json was written to GCF_021729145.1_ASM2172914v1_genomic.fna/dqc_result.json
[2024-01-24 13:57:52,786] [INFO] DFAST_QC completed!
[2024-01-24 13:57:52,786] [INFO] Total running time: 0h2m12s