[2024-01-25 17:44:50,423] [INFO] DFAST_QC pipeline started.
[2024-01-25 17:44:50,424] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 17:44:50,424] [INFO] DQC Reference Directory: /var/lib/cwl/stg9f1703b0-8a02-46fc-aed0-ba0d68c9364c/dqc_reference
[2024-01-25 17:44:51,545] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 17:44:51,546] [INFO] Task started: Prodigal
[2024-01-25 17:44:51,546] [INFO] Running command: gunzip -c /var/lib/cwl/stg8e032bc8-6bb5-4d96-b7db-4a1d8f11f861/GCF_022496575.1_ASM2249657v1_genomic.fna.gz | prodigal -d GCF_022496575.1_ASM2249657v1_genomic.fna/cds.fna -a GCF_022496575.1_ASM2249657v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 17:45:08,457] [INFO] Task succeeded: Prodigal
[2024-01-25 17:45:08,458] [INFO] Task started: HMMsearch
[2024-01-25 17:45:08,458] [INFO] Running command: hmmsearch --tblout GCF_022496575.1_ASM2249657v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg9f1703b0-8a02-46fc-aed0-ba0d68c9364c/dqc_reference/reference_markers.hmm GCF_022496575.1_ASM2249657v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 17:45:08,770] [INFO] Task succeeded: HMMsearch
[2024-01-25 17:45:08,772] [INFO] Found 6/6 markers.
[2024-01-25 17:45:08,821] [INFO] Query marker FASTA was written to GCF_022496575.1_ASM2249657v1_genomic.fna/markers.fasta
[2024-01-25 17:45:08,822] [INFO] Task started: Blastn
[2024-01-25 17:45:08,822] [INFO] Running command: blastn -query GCF_022496575.1_ASM2249657v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9f1703b0-8a02-46fc-aed0-ba0d68c9364c/dqc_reference/reference_markers.fasta -out GCF_022496575.1_ASM2249657v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 17:45:09,856] [INFO] Task succeeded: Blastn
[2024-01-25 17:45:09,859] [INFO] Selected 10 target genomes.
[2024-01-25 17:45:09,860] [INFO] Target genome list was writen to GCF_022496575.1_ASM2249657v1_genomic.fna/target_genomes.txt
[2024-01-25 17:45:09,870] [INFO] Task started: fastANI
[2024-01-25 17:45:09,870] [INFO] Running command: fastANI --query /var/lib/cwl/stg8e032bc8-6bb5-4d96-b7db-4a1d8f11f861/GCF_022496575.1_ASM2249657v1_genomic.fna.gz --refList GCF_022496575.1_ASM2249657v1_genomic.fna/target_genomes.txt --output GCF_022496575.1_ASM2249657v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 17:45:24,176] [INFO] Task succeeded: fastANI
[2024-01-25 17:45:24,177] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg9f1703b0-8a02-46fc-aed0-ba0d68c9364c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 17:45:24,177] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg9f1703b0-8a02-46fc-aed0-ba0d68c9364c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 17:45:24,184] [INFO] Found 10 fastANI hits (7 hits with ANI > threshold)
[2024-01-25 17:45:24,184] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 17:45:24,184] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Pseudomonas aeruginosa	strain=JCM 5962	GCA_022496575.1	287	287	type	True	100.0	2066	2066	95	conclusive
Pseudomonas aeruginosa	strain=DSM 50071	GCA_001042925.1	287	287	type	True	99.338	2004	2066	95	conclusive
Pseudomonas aeruginosa	strain=DSM 50071	GCA_024169805.1	287	287	type	True	99.3367	2019	2066	95	conclusive
Pseudomonas aeruginosa	strain=DSM 50071	GCA_012987025.1	287	287	type	True	99.3256	2005	2066	95	conclusive
Pseudomonas aeruginosa	strain=DSM 50071	GCA_900167195.1	287	287	type	True	99.3115	1981	2066	95	conclusive
Pseudomonas aeruginosa	strain=NCTC10332	GCA_001457615.1	287	287	type	True	99.282	2021	2066	95	conclusive
Pseudomonas aeruginosa	strain=CCUG 551	GCA_008801675.1	287	287	type	True	99.2594	1986	2066	95	conclusive
Pseudomonas lalucatii	strain=R1b54	GCA_018398425.1	1424203	1424203	type	True	82.6424	927	2066	95	below_threshold
Pseudomonas hydrolytica	strain=DSWY01	GCA_021495345.2	2493633	2493633	type	True	81.8239	991	2066	95	below_threshold
Pseudomonas yangonensis	strain=MY50	GCA_009932725.1	2579922	2579922	type	True	81.1597	929	2066	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 17:45:24,186] [INFO] DFAST Taxonomy check result was written to GCF_022496575.1_ASM2249657v1_genomic.fna/tc_result.tsv
[2024-01-25 17:45:24,186] [INFO] ===== Taxonomy check completed =====
[2024-01-25 17:45:24,186] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 17:45:24,186] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg9f1703b0-8a02-46fc-aed0-ba0d68c9364c/dqc_reference/checkm_data
[2024-01-25 17:45:24,187] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 17:45:24,244] [INFO] Task started: CheckM
[2024-01-25 17:45:24,244] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_022496575.1_ASM2249657v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_022496575.1_ASM2249657v1_genomic.fna/checkm_input GCF_022496575.1_ASM2249657v1_genomic.fna/checkm_result
[2024-01-25 17:46:16,617] [INFO] Task succeeded: CheckM
[2024-01-25 17:46:16,618] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 17:46:16,640] [INFO] ===== Completeness check finished =====
[2024-01-25 17:46:16,641] [INFO] ===== Start GTDB Search =====
[2024-01-25 17:46:16,641] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_022496575.1_ASM2249657v1_genomic.fna/markers.fasta)
[2024-01-25 17:46:16,642] [INFO] Task started: Blastn
[2024-01-25 17:46:16,642] [INFO] Running command: blastn -query GCF_022496575.1_ASM2249657v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9f1703b0-8a02-46fc-aed0-ba0d68c9364c/dqc_reference/reference_markers_gtdb.fasta -out GCF_022496575.1_ASM2249657v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 17:46:18,766] [INFO] Task succeeded: Blastn
[2024-01-25 17:46:18,768] [INFO] Selected 18 target genomes.
[2024-01-25 17:46:18,768] [INFO] Target genome list was writen to GCF_022496575.1_ASM2249657v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 17:46:18,796] [INFO] Task started: fastANI
[2024-01-25 17:46:18,796] [INFO] Running command: fastANI --query /var/lib/cwl/stg8e032bc8-6bb5-4d96-b7db-4a1d8f11f861/GCF_022496575.1_ASM2249657v1_genomic.fna.gz --refList GCF_022496575.1_ASM2249657v1_genomic.fna/target_genomes_gtdb.txt --output GCF_022496575.1_ASM2249657v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 17:46:43,328] [INFO] Task succeeded: fastANI
[2024-01-25 17:46:43,339] [INFO] Found 18 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 17:46:43,339] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001457615.1	s__Pseudomonas aeruginosa	99.2906	2020	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas	95.0	99.12	97.38	0.94	0.84	5623	conclusive
GCF_000017205.1	s__Pseudomonas aeruginosa_A	93.9826	1858	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas	95.0	99.07	98.78	0.93	0.80	42	-
GCF_900187975.1	s__Pseudomonas delhiensis	84.69	1356	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas	95.078	98.79	97.59	0.94	0.89	3	-
GCF_900103845.1	s__Pseudomonas jinjuensis	84.6174	1158	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas	95.0	99.99	99.99	1.00	1.00	2	-
GCF_900112375.1	s__Pseudomonas citronellolis	84.5765	1352	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas	95.078	97.44	97.08	0.86	0.82	26	-
GCF_017589465.1	s__Pseudomonas sp017589465	84.0934	1117	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014204515.1	s__Pseudomonas nitritireducens_A	83.8768	1310	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_017491605.1	s__Pseudomonas sp017491605	83.8544	1219	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002452115.1	s__Pseudomonas nitroreducens_B	83.8318	1121	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002091755.1	s__Pseudomonas nitroreducens	83.6007	1256	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas	95.0	98.47	95.19	0.93	0.83	16	-
GCF_904061905.1	s__Pseudomonas_E carbonaria	83.1292	1090	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003205495.1	s__Pseudomonas_E alcaligenes_B	82.8193	921	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_012767755.2	s__Pseudomonas_F sp003234055	82.8189	1247	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_F	95.0	96.89	96.11	0.89	0.88	3	-
GCF_003696305.1	s__Pseudomonas_E sp003696305	82.7078	1011	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002483005.1	s__Pseudomonas_E sp002483005	82.3808	562	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004801855.1	s__Pseudomonas_K sp004801855	82.3568	756	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_K	95.0	98.38	98.38	0.89	0.89	2	-
GCF_900105885.1	s__Pseudomonas_K guangdongensis	82.2207	714	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_K	95.0	98.99	98.99	0.99	0.99	2	-
GCF_900104805.1	s__Pseudomonas_K oryzae	82.1429	861	2066	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_K	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 17:46:43,340] [INFO] GTDB search result was written to GCF_022496575.1_ASM2249657v1_genomic.fna/result_gtdb.tsv
[2024-01-25 17:46:43,341] [INFO] ===== GTDB Search completed =====
[2024-01-25 17:46:43,344] [INFO] DFAST_QC result json was written to GCF_022496575.1_ASM2249657v1_genomic.fna/dqc_result.json
[2024-01-25 17:46:43,344] [INFO] DFAST_QC completed!
[2024-01-25 17:46:43,344] [INFO] Total running time: 0h1m53s