[2024-01-25 18:07:20,652] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:07:20,653] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:07:20,654] [INFO] DQC Reference Directory: /var/lib/cwl/stgc7f17b51-1bc4-47fa-ba2f-4c2e2b188d30/dqc_reference
[2024-01-25 18:07:21,827] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:07:21,828] [INFO] Task started: Prodigal
[2024-01-25 18:07:21,828] [INFO] Running command: gunzip -c /var/lib/cwl/stg81b62a89-a66d-48c5-a38f-baaf04478b8b/GCF_022549755.1_ASM2254975v1_genomic.fna.gz | prodigal -d GCF_022549755.1_ASM2254975v1_genomic.fna/cds.fna -a GCF_022549755.1_ASM2254975v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:07:38,075] [INFO] Task succeeded: Prodigal
[2024-01-25 18:07:38,076] [INFO] Task started: HMMsearch
[2024-01-25 18:07:38,076] [INFO] Running command: hmmsearch --tblout GCF_022549755.1_ASM2254975v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc7f17b51-1bc4-47fa-ba2f-4c2e2b188d30/dqc_reference/reference_markers.hmm GCF_022549755.1_ASM2254975v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:07:38,348] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:07:38,349] [INFO] Found 6/6 markers.
[2024-01-25 18:07:38,394] [INFO] Query marker FASTA was written to GCF_022549755.1_ASM2254975v1_genomic.fna/markers.fasta
[2024-01-25 18:07:38,394] [INFO] Task started: Blastn
[2024-01-25 18:07:38,394] [INFO] Running command: blastn -query GCF_022549755.1_ASM2254975v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc7f17b51-1bc4-47fa-ba2f-4c2e2b188d30/dqc_reference/reference_markers.fasta -out GCF_022549755.1_ASM2254975v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:07:38,957] [INFO] Task succeeded: Blastn
[2024-01-25 18:07:38,961] [INFO] Selected 8 target genomes.
[2024-01-25 18:07:38,962] [INFO] Target genome list was writen to GCF_022549755.1_ASM2254975v1_genomic.fna/target_genomes.txt
[2024-01-25 18:07:38,966] [INFO] Task started: fastANI
[2024-01-25 18:07:38,967] [INFO] Running command: fastANI --query /var/lib/cwl/stg81b62a89-a66d-48c5-a38f-baaf04478b8b/GCF_022549755.1_ASM2254975v1_genomic.fna.gz --refList GCF_022549755.1_ASM2254975v1_genomic.fna/target_genomes.txt --output GCF_022549755.1_ASM2254975v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:07:46,605] [INFO] Task succeeded: fastANI
[2024-01-25 18:07:46,605] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc7f17b51-1bc4-47fa-ba2f-4c2e2b188d30/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:07:46,605] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc7f17b51-1bc4-47fa-ba2f-4c2e2b188d30/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:07:46,611] [INFO] Found 8 fastANI hits (2 hits with ANI > threshold)
[2024-01-25 18:07:46,611] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:07:46,611] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Belliella aquatica	strain=JCM 19468	GCA_022549755.1	1323734	1323734	type	True	100.0	1514	1516	95	conclusive
Belliella aquatica	strain=CGMCC 1.12479	GCA_014637795.1	1323734	1323734	type	True	99.9767	1492	1516	95	conclusive
Belliella baltica	strain=DSM 15883	GCA_000265405.1	232259	232259	type	True	86.5312	977	1516	95	below_threshold
Belliella buryatensis	strain=5C	GCA_900188245.1	1500549	1500549	type	True	80.943	536	1516	95	below_threshold
Belliella pelovolcani	strain=DSM 46698	GCA_900156785.1	529505	529505	type	True	79.2328	487	1516	95	below_threshold
Aquiflexum lacus	strain=CUG 91378	GCA_015476655.1	2483805	2483805	type	True	77.1319	221	1516	95	below_threshold
Algoriphagus algorifonticola	strain=hg1	GCA_007655305.1	2593007	2593007	type	True	76.5322	117	1516	95	below_threshold
Algoriphagus ornithinivorans	strain=DSM 15282	GCA_900115305.1	226506	226506	type	True	76.3336	124	1516	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:07:46,613] [INFO] DFAST Taxonomy check result was written to GCF_022549755.1_ASM2254975v1_genomic.fna/tc_result.tsv
[2024-01-25 18:07:46,613] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:07:46,613] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:07:46,613] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc7f17b51-1bc4-47fa-ba2f-4c2e2b188d30/dqc_reference/checkm_data
[2024-01-25 18:07:46,614] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:07:46,658] [INFO] Task started: CheckM
[2024-01-25 18:07:46,659] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_022549755.1_ASM2254975v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_022549755.1_ASM2254975v1_genomic.fna/checkm_input GCF_022549755.1_ASM2254975v1_genomic.fna/checkm_result
[2024-01-25 18:08:36,127] [INFO] Task succeeded: CheckM
[2024-01-25 18:08:36,129] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:08:36,153] [INFO] ===== Completeness check finished =====
[2024-01-25 18:08:36,154] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:08:36,155] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_022549755.1_ASM2254975v1_genomic.fna/markers.fasta)
[2024-01-25 18:08:36,155] [INFO] Task started: Blastn
[2024-01-25 18:08:36,155] [INFO] Running command: blastn -query GCF_022549755.1_ASM2254975v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc7f17b51-1bc4-47fa-ba2f-4c2e2b188d30/dqc_reference/reference_markers_gtdb.fasta -out GCF_022549755.1_ASM2254975v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:08:36,980] [INFO] Task succeeded: Blastn
[2024-01-25 18:08:36,990] [INFO] Selected 13 target genomes.
[2024-01-25 18:08:36,991] [INFO] Target genome list was writen to GCF_022549755.1_ASM2254975v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:08:37,009] [INFO] Task started: fastANI
[2024-01-25 18:08:37,010] [INFO] Running command: fastANI --query /var/lib/cwl/stg81b62a89-a66d-48c5-a38f-baaf04478b8b/GCF_022549755.1_ASM2254975v1_genomic.fna.gz --refList GCF_022549755.1_ASM2254975v1_genomic.fna/target_genomes_gtdb.txt --output GCF_022549755.1_ASM2254975v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:08:48,524] [INFO] Task succeeded: fastANI
[2024-01-25 18:08:48,532] [INFO] Found 13 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:08:48,532] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014637795.1	s__Belliella aquatica	99.9767	1492	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Belliella	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_000265405.1	s__Belliella baltica	86.5776	973	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Belliella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900188245.1	s__Belliella buryatensis	80.943	536	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Belliella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900156785.1	s__Belliella pelovolcani	79.2289	489	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Belliella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014652135.1	s__Mongoliitalea lutea	77.4023	192	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Mongoliitalea	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003797895.1	s__Cecembia sp003797895	77.3572	176	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Cecembia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_015476655.1	s__Aquiflexum lacus	77.1325	221	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Aquiflexum	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003014575.1	s__Cecembia rubra	76.9148	156	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Cecembia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900112005.1	s__Algoriphagus aquimarinus	76.6423	112	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900148515.1	s__Algoriphagus zhangzhouensis	76.5839	137	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	100.00	100.00	1.00	1.00	2	-
GCF_007655305.1	s__Algoriphagus sp007655305	76.5322	117	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002150685.1	s__Algoriphagus antarcticus	76.3408	107	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	100.00	100.00	1.00	1.00	2	-
GCF_900115305.1	s__Algoriphagus ornithinivorans	76.3336	124	1516	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	95.91	95.63	0.88	0.85	7	-
--------------------------------------------------------------------------------
[2024-01-25 18:08:48,534] [INFO] GTDB search result was written to GCF_022549755.1_ASM2254975v1_genomic.fna/result_gtdb.tsv
[2024-01-25 18:08:48,534] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:08:48,537] [INFO] DFAST_QC result json was written to GCF_022549755.1_ASM2254975v1_genomic.fna/dqc_result.json
[2024-01-25 18:08:48,537] [INFO] DFAST_QC completed!
[2024-01-25 18:08:48,537] [INFO] Total running time: 0h1m28s