[2024-01-25 18:06:20,635] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:06:20,639] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:06:20,639] [INFO] DQC Reference Directory: /var/lib/cwl/stgc0a1454a-7e88-458e-b76c-51ef03f2353d/dqc_reference
[2024-01-25 18:06:21,755] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:06:21,755] [INFO] Task started: Prodigal
[2024-01-25 18:06:21,756] [INFO] Running command: gunzip -c /var/lib/cwl/stg0eee0668-d213-408b-9544-8c6e1e3adc3a/GCF_022870045.1_ASM2287004v1_genomic.fna.gz | prodigal -d GCF_022870045.1_ASM2287004v1_genomic.fna/cds.fna -a GCF_022870045.1_ASM2287004v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:06:29,228] [INFO] Task succeeded: Prodigal
[2024-01-25 18:06:29,228] [INFO] Task started: HMMsearch
[2024-01-25 18:06:29,228] [INFO] Running command: hmmsearch --tblout GCF_022870045.1_ASM2287004v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc0a1454a-7e88-458e-b76c-51ef03f2353d/dqc_reference/reference_markers.hmm GCF_022870045.1_ASM2287004v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:06:29,454] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:06:29,455] [INFO] Found 6/6 markers.
[2024-01-25 18:06:29,486] [INFO] Query marker FASTA was written to GCF_022870045.1_ASM2287004v1_genomic.fna/markers.fasta
[2024-01-25 18:06:29,487] [INFO] Task started: Blastn
[2024-01-25 18:06:29,487] [INFO] Running command: blastn -query GCF_022870045.1_ASM2287004v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc0a1454a-7e88-458e-b76c-51ef03f2353d/dqc_reference/reference_markers.fasta -out GCF_022870045.1_ASM2287004v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:06:30,075] [INFO] Task succeeded: Blastn
[2024-01-25 18:06:30,079] [INFO] Selected 8 target genomes.
[2024-01-25 18:06:30,079] [INFO] Target genome list was writen to GCF_022870045.1_ASM2287004v1_genomic.fna/target_genomes.txt
[2024-01-25 18:06:30,086] [INFO] Task started: fastANI
[2024-01-25 18:06:30,086] [INFO] Running command: fastANI --query /var/lib/cwl/stg0eee0668-d213-408b-9544-8c6e1e3adc3a/GCF_022870045.1_ASM2287004v1_genomic.fna.gz --refList GCF_022870045.1_ASM2287004v1_genomic.fna/target_genomes.txt --output GCF_022870045.1_ASM2287004v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:06:37,620] [INFO] Task succeeded: fastANI
[2024-01-25 18:06:37,620] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc0a1454a-7e88-458e-b76c-51ef03f2353d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:06:37,620] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc0a1454a-7e88-458e-b76c-51ef03f2353d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:06:37,626] [INFO] Found 8 fastANI hits (5 hits with ANI > threshold)
[2024-01-25 18:06:37,626] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:06:37,627] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Acinetobacter baumannii	strain=PartI-Abaumannii-RM8376	GCA_022870045.1	470	470	type	True	100.0	1323	1326	95	conclusive
Acinetobacter baumannii	strain=ATCC 19606	GCA_020911985.1	470	470	type	True	99.9999	1321	1326	95	conclusive
Acinetobacter baumannii	strain=ATCC 19606	GCA_009035845.1	470	470	type	True	99.9979	1322	1326	95	conclusive
Acinetobacter baumannii	strain=ATCC 19606	GCA_000737145.1	470	470	type	True	99.9853	1309	1326	95	conclusive
Acinetobacter baumannii	strain=ATCC 19606	GCA_014116795.1	470	470	type	True	99.9836	1294	1326	95	conclusive
Acinetobacter pittii	strain=CIP70.29	GCA_024390955.1	48296	48296	type	True	89.1075	1052	1326	95	below_threshold
Acinetobacter junii	strain=NCTC10307	GCA_900444875.1	40215	40215	type	True	80.6394	467	1326	95	below_threshold
Acinetobacter pecorum	strain=Sa1BUA6	GCA_014837015.1	2762215	2762215	type	True	78.6875	289	1326	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:06:37,628] [INFO] DFAST Taxonomy check result was written to GCF_022870045.1_ASM2287004v1_genomic.fna/tc_result.tsv
[2024-01-25 18:06:37,628] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:06:37,628] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:06:37,629] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc0a1454a-7e88-458e-b76c-51ef03f2353d/dqc_reference/checkm_data
[2024-01-25 18:06:37,629] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:06:37,671] [INFO] Task started: CheckM
[2024-01-25 18:06:37,671] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_022870045.1_ASM2287004v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_022870045.1_ASM2287004v1_genomic.fna/checkm_input GCF_022870045.1_ASM2287004v1_genomic.fna/checkm_result
[2024-01-25 18:07:04,287] [INFO] Task succeeded: CheckM
[2024-01-25 18:07:04,287] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:07:04,302] [INFO] ===== Completeness check finished =====
[2024-01-25 18:07:04,302] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:07:04,302] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_022870045.1_ASM2287004v1_genomic.fna/markers.fasta)
[2024-01-25 18:07:04,302] [INFO] Task started: Blastn
[2024-01-25 18:07:04,302] [INFO] Running command: blastn -query GCF_022870045.1_ASM2287004v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc0a1454a-7e88-458e-b76c-51ef03f2353d/dqc_reference/reference_markers_gtdb.fasta -out GCF_022870045.1_ASM2287004v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:07:05,168] [INFO] Task succeeded: Blastn
[2024-01-25 18:07:05,172] [INFO] Selected 11 target genomes.
[2024-01-25 18:07:05,173] [INFO] Target genome list was writen to GCF_022870045.1_ASM2287004v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:07:05,186] [INFO] Task started: fastANI
[2024-01-25 18:07:05,187] [INFO] Running command: fastANI --query /var/lib/cwl/stg0eee0668-d213-408b-9544-8c6e1e3adc3a/GCF_022870045.1_ASM2287004v1_genomic.fna.gz --refList GCF_022870045.1_ASM2287004v1_genomic.fna/target_genomes_gtdb.txt --output GCF_022870045.1_ASM2287004v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:07:15,850] [INFO] Task succeeded: fastANI
[2024-01-25 18:07:15,857] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:07:15,858] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_009759685.1	s__Acinetobacter baumannii	99.9984	1320	1326	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.86	96.67	0.89	0.80	5417	conclusive
GCF_000368085.1	s__Acinetobacter nosocomialis	92.0212	1106	1326	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.94	97.50	0.90	0.85	250	-
GCF_000368065.1	s__Acinetobacter seifertii	90.2688	1060	1326	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	96.88	96.48	0.87	0.81	81	-
GCF_002928115.1	s__Acinetobacter pittii_H	89.2906	1077	1326	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	96.46	96.15	0.91	0.87	9	-
GCF_001605885.1	s__Acinetobacter lactucae	88.58	1059	1326	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.34	96.55	0.91	0.85	16	-
GCF_013344765.1	s__Acinetobacter lactucae_A	88.439	874	1326	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.51	97.14	0.90	0.87	4	-
GCF_000399685.1	s__Acinetobacter pittii_E	88.4039	1071	1326	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000196795.1	s__Acinetobacter oleivorans	88.2065	1087	1326	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	96.22	95.12	0.90	0.85	40	-
GCF_000313935.1	s__Acinetobacter sp000313935	88.0889	1039	1326	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	99.37	99.37	0.94	0.94	2	-
GCF_000399665.1	s__Acinetobacter calcoaceticus_B	87.7999	1047	1326	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016508255.1	s__Acinetobacter calcoaceticus_E	87.2541	1034	1326	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 18:07:15,859] [INFO] GTDB search result was written to GCF_022870045.1_ASM2287004v1_genomic.fna/result_gtdb.tsv
[2024-01-25 18:07:15,859] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:07:15,862] [INFO] DFAST_QC result json was written to GCF_022870045.1_ASM2287004v1_genomic.fna/dqc_result.json
[2024-01-25 18:07:15,862] [INFO] DFAST_QC completed!
[2024-01-25 18:07:15,862] [INFO] Total running time: 0h0m55s