[2024-01-24 11:19:54,285] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:19:54,287] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:19:54,287] [INFO] DQC Reference Directory: /var/lib/cwl/stg519b2e84-52d1-47b9-b81e-46bdec86c285/dqc_reference
[2024-01-24 11:19:55,726] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:19:55,728] [INFO] Task started: Prodigal
[2024-01-24 11:19:55,728] [INFO] Running command: gunzip -c /var/lib/cwl/stg313ae425-1b63-4c60-8b23-54e4fffe18eb/GCF_024138145.1_ASM2413814v1_genomic.fna.gz | prodigal -d GCF_024138145.1_ASM2413814v1_genomic.fna/cds.fna -a GCF_024138145.1_ASM2413814v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:20:14,368] [INFO] Task succeeded: Prodigal
[2024-01-24 11:20:14,369] [INFO] Task started: HMMsearch
[2024-01-24 11:20:14,369] [INFO] Running command: hmmsearch --tblout GCF_024138145.1_ASM2413814v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg519b2e84-52d1-47b9-b81e-46bdec86c285/dqc_reference/reference_markers.hmm GCF_024138145.1_ASM2413814v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:20:14,649] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:20:14,651] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg313ae425-1b63-4c60-8b23-54e4fffe18eb/GCF_024138145.1_ASM2413814v1_genomic.fna.gz]
[2024-01-24 11:20:14,696] [INFO] Query marker FASTA was written to GCF_024138145.1_ASM2413814v1_genomic.fna/markers.fasta
[2024-01-24 11:20:14,697] [INFO] Task started: Blastn
[2024-01-24 11:20:14,697] [INFO] Running command: blastn -query GCF_024138145.1_ASM2413814v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg519b2e84-52d1-47b9-b81e-46bdec86c285/dqc_reference/reference_markers.fasta -out GCF_024138145.1_ASM2413814v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:20:15,245] [INFO] Task succeeded: Blastn
[2024-01-24 11:20:15,249] [INFO] Selected 11 target genomes.
[2024-01-24 11:20:15,249] [INFO] Target genome list was writen to GCF_024138145.1_ASM2413814v1_genomic.fna/target_genomes.txt
[2024-01-24 11:20:15,255] [INFO] Task started: fastANI
[2024-01-24 11:20:15,256] [INFO] Running command: fastANI --query /var/lib/cwl/stg313ae425-1b63-4c60-8b23-54e4fffe18eb/GCF_024138145.1_ASM2413814v1_genomic.fna.gz --refList GCF_024138145.1_ASM2413814v1_genomic.fna/target_genomes.txt --output GCF_024138145.1_ASM2413814v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:20:24,349] [INFO] Task succeeded: fastANI
[2024-01-24 11:20:24,350] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg519b2e84-52d1-47b9-b81e-46bdec86c285/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:20:24,350] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg519b2e84-52d1-47b9-b81e-46bdec86c285/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:20:24,360] [INFO] Found 11 fastANI hits (0 hits with ANI > threshold)
[2024-01-24 11:20:24,360] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-24 11:20:24,360] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Natronococcus jeotgali	strain=DSM 18795	GCA_000337695.1	413812	413812	type	True	79.381	531	1283	95	below_threshold
Natronococcus pandeyae	strain=LS1_42	GCA_008122205.1	2055836	2055836	type	True	79.1957	578	1283	95	below_threshold
Natrinema versiforme	strain=JCM 10478	GCA_000337195.1	88724	88724	type	True	79.1408	499	1283	95	below_threshold
Halopiger salifodinae	strain=CGMCC 1.12284	GCA_900110455.1	1202768	1202768	type	True	79.052	545	1283	95	below_threshold
Natrinema amylolyticum	strain=LT61	GCA_020515625.1	2878679	2878679	type	True	78.9985	533	1283	95	below_threshold
Halopiger salifodinae	strain=KCY07-B2	GCA_000784335.1	1202768	1202768	type	True	78.9551	537	1283	95	below_threshold
Natrinema salaciae	strain=DSM 25055	GCA_900110865.1	1186196	1186196	type	True	78.9516	542	1283	95	below_threshold
Natrinema pallidum	strain=DSM 3751	GCA_000337615.1	69527	69527	type	True	78.8829	455	1283	95	below_threshold
Natrinema altunense	strain=AJ2	GCA_000731985.1	222984	222984	type	True	78.8682	469	1283	95	below_threshold
Haloterrigena hispanica	strain=DSM 18328	GCA_004217335.1	392421	392421	type	True	78.8264	464	1283	95	below_threshold
Natrinema altunense	strain=JCM 12890	GCA_000337155.1	222984	222984	type	True	78.8169	461	1283	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:20:24,362] [INFO] DFAST Taxonomy check result was written to GCF_024138145.1_ASM2413814v1_genomic.fna/tc_result.tsv
[2024-01-24 11:20:24,362] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:20:24,362] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:20:24,363] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg519b2e84-52d1-47b9-b81e-46bdec86c285/dqc_reference/checkm_data
[2024-01-24 11:20:24,364] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:20:24,408] [INFO] Task started: CheckM
[2024-01-24 11:20:24,408] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_024138145.1_ASM2413814v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_024138145.1_ASM2413814v1_genomic.fna/checkm_input GCF_024138145.1_ASM2413814v1_genomic.fna/checkm_result
[2024-01-24 11:21:16,230] [INFO] Task succeeded: CheckM
[2024-01-24 11:21:16,232] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:21:16,255] [INFO] ===== Completeness check finished =====
[2024-01-24 11:21:16,256] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:21:16,256] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_024138145.1_ASM2413814v1_genomic.fna/markers.fasta)
[2024-01-24 11:21:16,257] [INFO] Task started: Blastn
[2024-01-24 11:21:16,257] [INFO] Running command: blastn -query GCF_024138145.1_ASM2413814v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg519b2e84-52d1-47b9-b81e-46bdec86c285/dqc_reference/reference_markers_gtdb.fasta -out GCF_024138145.1_ASM2413814v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:21:16,779] [INFO] Task succeeded: Blastn
[2024-01-24 11:21:16,783] [INFO] Selected 11 target genomes.
[2024-01-24 11:21:16,783] [INFO] Target genome list was writen to GCF_024138145.1_ASM2413814v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:21:16,790] [INFO] Task started: fastANI
[2024-01-24 11:21:16,790] [INFO] Running command: fastANI --query /var/lib/cwl/stg313ae425-1b63-4c60-8b23-54e4fffe18eb/GCF_024138145.1_ASM2413814v1_genomic.fna.gz --refList GCF_024138145.1_ASM2413814v1_genomic.fna/target_genomes_gtdb.txt --output GCF_024138145.1_ASM2413814v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:21:26,216] [INFO] Task succeeded: fastANI
[2024-01-24 11:21:26,232] [INFO] Found 11 fastANI hits (0 hits with ANI > circumscription radius)
[2024-01-24 11:21:26,232] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_017357105.1	s__Tc-Br11 sp017357105	91.7214	1034	1283	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Tc-Br11	95.0	N/A	N/A	N/A	N/A	1	-
GCA_001564275.1	s__Tc-Br11 sp001564275	79.5866	499	1283	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Tc-Br11	95.0	99.79	99.79	0.91	0.91	2	-
GCF_001953745.1	s__Natrinema saccharevitans	79.4248	517	1283	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	N/A	N/A	N/A	N/A	1	-
GCF_008122205.1	s__Natronococcus sp008122205	79.2147	576	1283	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natronococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000337195.1	s__Natrinema versiforme	79.1232	502	1283	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	N/A	N/A	N/A	N/A	1	-
GCF_005576615.1	s__Natrinema versiforme_A	79.0884	554	1283	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900110455.1	s__Natrinema salifodinae	79.0322	547	1283	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	100.00	100.00	0.98	0.98	2	-
GCA_003559215.1	s__Natronococcus sp003559215	79.0188	526	1283	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natronococcus	95.0	99.83	99.81	0.98	0.98	4	-
GCF_900110865.1	s__Natrinema salaciae	78.944	543	1283	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000337615.1	s__Natrinema pallidum	78.8689	457	1283	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	98.94	98.94	0.86	0.86	2	-
GCF_000731985.1	s__Natrinema altunense	78.8545	471	1283	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	98.98	98.00	0.96	0.93	4	-
--------------------------------------------------------------------------------
[2024-01-24 11:21:26,234] [INFO] GTDB search result was written to GCF_024138145.1_ASM2413814v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:21:26,234] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:21:26,238] [INFO] DFAST_QC result json was written to GCF_024138145.1_ASM2413814v1_genomic.fna/dqc_result.json
[2024-01-24 11:21:26,238] [INFO] DFAST_QC completed!
[2024-01-24 11:21:26,238] [INFO] Total running time: 0h1m32s