[2024-01-25 20:25:50,449] [INFO] DFAST_QC pipeline started.
[2024-01-25 20:25:50,451] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 20:25:50,451] [INFO] DQC Reference Directory: /var/lib/cwl/stg60b63240-1db6-4350-8cba-472f2a79cc3e/dqc_reference
[2024-01-25 20:25:51,603] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 20:25:51,604] [INFO] Task started: Prodigal
[2024-01-25 20:25:51,604] [INFO] Running command: gunzip -c /var/lib/cwl/stg60538900-d44c-44c8-948f-6ba654567d15/GCF_024347655.1_ASM2434765v1_genomic.fna.gz | prodigal -d GCF_024347655.1_ASM2434765v1_genomic.fna/cds.fna -a GCF_024347655.1_ASM2434765v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 20:26:01,534] [INFO] Task succeeded: Prodigal
[2024-01-25 20:26:01,534] [INFO] Task started: HMMsearch
[2024-01-25 20:26:01,534] [INFO] Running command: hmmsearch --tblout GCF_024347655.1_ASM2434765v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg60b63240-1db6-4350-8cba-472f2a79cc3e/dqc_reference/reference_markers.hmm GCF_024347655.1_ASM2434765v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 20:26:01,756] [INFO] Task succeeded: HMMsearch
[2024-01-25 20:26:01,757] [INFO] Found 6/6 markers.
[2024-01-25 20:26:01,791] [INFO] Query marker FASTA was written to GCF_024347655.1_ASM2434765v1_genomic.fna/markers.fasta
[2024-01-25 20:26:01,791] [INFO] Task started: Blastn
[2024-01-25 20:26:01,791] [INFO] Running command: blastn -query GCF_024347655.1_ASM2434765v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg60b63240-1db6-4350-8cba-472f2a79cc3e/dqc_reference/reference_markers.fasta -out GCF_024347655.1_ASM2434765v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:26:02,526] [INFO] Task succeeded: Blastn
[2024-01-25 20:26:02,528] [INFO] Selected 11 target genomes.
[2024-01-25 20:26:02,528] [INFO] Target genome list was writen to GCF_024347655.1_ASM2434765v1_genomic.fna/target_genomes.txt
[2024-01-25 20:26:02,554] [INFO] Task started: fastANI
[2024-01-25 20:26:02,555] [INFO] Running command: fastANI --query /var/lib/cwl/stg60538900-d44c-44c8-948f-6ba654567d15/GCF_024347655.1_ASM2434765v1_genomic.fna.gz --refList GCF_024347655.1_ASM2434765v1_genomic.fna/target_genomes.txt --output GCF_024347655.1_ASM2434765v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 20:26:16,562] [INFO] Task succeeded: fastANI
[2024-01-25 20:26:16,562] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg60b63240-1db6-4350-8cba-472f2a79cc3e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 20:26:16,563] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg60b63240-1db6-4350-8cba-472f2a79cc3e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 20:26:16,570] [INFO] Found 11 fastANI hits (5 hits with ANI > threshold)
[2024-01-25 20:26:16,570] [INFO] The taxonomy check result is classified as 'inconclusive'.
[2024-01-25 20:26:16,570] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Vibrio toranzoniae	strain=CECT 7225	GCA_024347655.1	1194427	1194427	type	True	100.0	1530	1534	95	inconclusive
Vibrio toranzoniae	strain=CECT 7225	GCA_900089765.1	1194427	1194427	type	True	99.9847	1496	1534	95	inconclusive
Vibrio toranzoniae	strain=Vb 10.8	GCA_001541335.1	1194427	1194427	type	True	99.9587	1466	1534	95	inconclusive
Vibrio kanaloae	strain=LMG 20539	GCA_024347535.1	170673	170673	type	True	95.4097	1362	1534	95	inconclusive
Vibrio kanaloae	strain=CCUG 56968	GCA_008801285.1	170673	170673	type	True	95.3516	1339	1534	95	inconclusive
Vibrio pomeroyi	strain=LMG 20537	GCA_024347595.1	198832	198832	type	True	89.6208	1279	1534	95	below_threshold
Vibrio celticus	strain=CECT 7224	GCA_024347335.1	446372	446372	type	True	88.4467	1249	1534	95	below_threshold
Vibrio coralliirubri	strain=DSM 27495	GCA_024347375.1	1516159	1516159	type	True	88.3217	1257	1534	95	below_threshold
Vibrio gigantis	strain=LMG 22741	GCA_024347515.1	296199	296199	type	True	88.2365	1244	1534	95	below_threshold
Vibrio gigantis	strain=LGP 13	GCA_002156475.1	296199	296199	type	True	88.1153	1202	1534	95	below_threshold
Vibrio bathopelagicus	strain=Sal10	GCA_014879975.1	2777577	2777577	type	True	88.0348	1244	1534	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 20:26:16,572] [INFO] DFAST Taxonomy check result was written to GCF_024347655.1_ASM2434765v1_genomic.fna/tc_result.tsv
[2024-01-25 20:26:16,572] [INFO] ===== Taxonomy check completed =====
[2024-01-25 20:26:16,572] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 20:26:16,572] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg60b63240-1db6-4350-8cba-472f2a79cc3e/dqc_reference/checkm_data
[2024-01-25 20:26:16,573] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 20:26:16,618] [INFO] Task started: CheckM
[2024-01-25 20:26:16,619] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_024347655.1_ASM2434765v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_024347655.1_ASM2434765v1_genomic.fna/checkm_input GCF_024347655.1_ASM2434765v1_genomic.fna/checkm_result
[2024-01-25 20:26:50,298] [INFO] Task succeeded: CheckM
[2024-01-25 20:26:50,299] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 20:26:50,317] [INFO] ===== Completeness check finished =====
[2024-01-25 20:26:50,317] [INFO] ===== Start GTDB Search =====
[2024-01-25 20:26:50,317] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_024347655.1_ASM2434765v1_genomic.fna/markers.fasta)
[2024-01-25 20:26:50,318] [INFO] Task started: Blastn
[2024-01-25 20:26:50,318] [INFO] Running command: blastn -query GCF_024347655.1_ASM2434765v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg60b63240-1db6-4350-8cba-472f2a79cc3e/dqc_reference/reference_markers_gtdb.fasta -out GCF_024347655.1_ASM2434765v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:26:51,406] [INFO] Task succeeded: Blastn
[2024-01-25 20:26:51,408] [INFO] Selected 15 target genomes.
[2024-01-25 20:26:51,408] [INFO] Target genome list was writen to GCF_024347655.1_ASM2434765v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 20:26:51,428] [INFO] Task started: fastANI
[2024-01-25 20:26:51,428] [INFO] Running command: fastANI --query /var/lib/cwl/stg60538900-d44c-44c8-948f-6ba654567d15/GCF_024347655.1_ASM2434765v1_genomic.fna.gz --refList GCF_024347655.1_ASM2434765v1_genomic.fna/target_genomes_gtdb.txt --output GCF_024347655.1_ASM2434765v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 20:27:09,512] [INFO] Task succeeded: fastANI
[2024-01-25 20:27:09,522] [INFO] Found 15 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 20:27:09,522] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_900089765.1	s__Vibrio toranzoniae	99.9847	1496	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.372	98.37	97.69	0.93	0.89	7	conclusive
GCF_008801285.1	s__Vibrio kanaloae	95.362	1338	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.372	98.50	98.26	0.91	0.88	16	-
GCF_013394185.1	s__Vibrio sp013394185	89.728	1271	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.0	100.00	100.00	0.99	0.99	2	-
GCF_000316985.1	s__Vibrio sp000316985	89.2685	1163	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001048535.1	s__Vibrio crassostreae	88.6746	1242	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.0	97.86	96.20	0.91	0.83	177	-
GCF_000272105.2	s__Vibrio splendidus_I	88.515	1201	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002156455.1	s__Vibrio coralliirubri	88.407	1169	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.2787	96.55	95.77	0.88	0.83	22	-
GCF_007858795.1	s__Vibrio sp007858795	88.3714	1236	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002156525.1	s__Vibrio celticus	88.3079	1205	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.2787	99.99	99.99	1.00	1.00	2	-
GCF_002875945.1	s__Vibrio lentus_A	88.2804	1237	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002156475.1	s__Vibrio gigantis	88.1153	1202	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.0	98.72	97.43	0.95	0.90	3	-
GCF_014879975.1	s__Vibrio bathopelagicus	88.0346	1244	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014878155.1	s__Vibrio lentus	88.0075	1224	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.0	96.60	95.56	0.86	0.83	91	-
GCF_002875705.1	s__Vibrio sp002875705	87.8343	1181	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	95.0	99.93	99.66	0.96	0.93	9	-
GCF_000272405.2	s__Vibrio tasmaniensis_A	87.5827	1128	1534	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Vibrionaceae;g__Vibrio	96.5791	97.23	97.01	0.87	0.85	20	-
--------------------------------------------------------------------------------
[2024-01-25 20:27:09,525] [INFO] GTDB search result was written to GCF_024347655.1_ASM2434765v1_genomic.fna/result_gtdb.tsv
[2024-01-25 20:27:09,525] [INFO] ===== GTDB Search completed =====
[2024-01-25 20:27:09,528] [INFO] DFAST_QC result json was written to GCF_024347655.1_ASM2434765v1_genomic.fna/dqc_result.json
[2024-01-25 20:27:09,528] [INFO] DFAST_QC completed!
[2024-01-25 20:27:09,528] [INFO] Total running time: 0h1m19s