[2024-01-24 14:23:05,270] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:23:05,272] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:23:05,272] [INFO] DQC Reference Directory: /var/lib/cwl/stge7515855-2ef1-43f3-8e82-1f314474c219/dqc_reference
[2024-01-24 14:23:06,614] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:23:06,615] [INFO] Task started: Prodigal
[2024-01-24 14:23:06,615] [INFO] Running command: gunzip -c /var/lib/cwl/stg0a6cf6f1-3df4-4db6-a8e2-7abcef042b84/GCF_024390995.1_ASM2439099v1_genomic.fna.gz | prodigal -d GCF_024390995.1_ASM2439099v1_genomic.fna/cds.fna -a GCF_024390995.1_ASM2439099v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:23:19,975] [INFO] Task succeeded: Prodigal
[2024-01-24 14:23:19,975] [INFO] Task started: HMMsearch
[2024-01-24 14:23:19,975] [INFO] Running command: hmmsearch --tblout GCF_024390995.1_ASM2439099v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stge7515855-2ef1-43f3-8e82-1f314474c219/dqc_reference/reference_markers.hmm GCF_024390995.1_ASM2439099v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:23:20,356] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:23:20,358] [INFO] Found 6/6 markers.
[2024-01-24 14:23:20,408] [INFO] Query marker FASTA was written to GCF_024390995.1_ASM2439099v1_genomic.fna/markers.fasta
[2024-01-24 14:23:20,408] [INFO] Task started: Blastn
[2024-01-24 14:23:20,409] [INFO] Running command: blastn -query GCF_024390995.1_ASM2439099v1_genomic.fna/markers.fasta -db /var/lib/cwl/stge7515855-2ef1-43f3-8e82-1f314474c219/dqc_reference/reference_markers.fasta -out GCF_024390995.1_ASM2439099v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:23:21,321] [INFO] Task succeeded: Blastn
[2024-01-24 14:23:21,325] [INFO] Selected 13 target genomes.
[2024-01-24 14:23:21,325] [INFO] Target genome list was writen to GCF_024390995.1_ASM2439099v1_genomic.fna/target_genomes.txt
[2024-01-24 14:23:21,331] [INFO] Task started: fastANI
[2024-01-24 14:23:21,331] [INFO] Running command: fastANI --query /var/lib/cwl/stg0a6cf6f1-3df4-4db6-a8e2-7abcef042b84/GCF_024390995.1_ASM2439099v1_genomic.fna.gz --refList GCF_024390995.1_ASM2439099v1_genomic.fna/target_genomes.txt --output GCF_024390995.1_ASM2439099v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:23:37,417] [INFO] Task succeeded: fastANI
[2024-01-24 14:23:37,417] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stge7515855-2ef1-43f3-8e82-1f314474c219/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:23:37,418] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stge7515855-2ef1-43f3-8e82-1f314474c219/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:23:37,431] [INFO] Found 13 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 14:23:37,431] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:23:37,431] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Enterobacter roggenkampii	strain=DSM 16690	GCA_024390995.1	1812935	1812935	type	True	100.0	1544	1552	95	conclusive
Enterobacter roggenkampii	strain=DSM 16690	GCA_001729805.1	1812935	1812935	type	True	98.5148	1354	1552	95	conclusive
Enterobacter roggenkampii	strain=FDAARGOS 1430	GCA_019047025.1	1812935	1812935	type	True	98.4709	1356	1552	95	conclusive
Enterobacter vonholyi	strain=E13	GCA_008364555.1	2797505	2797505	type	True	94.2992	1283	1552	95	below_threshold
Enterobacter asburiae	strain=ATCC 35953	GCA_001521715.1	61645	61645	type	True	93.2741	1284	1552	95	below_threshold
Enterobacter asburiae	strain=FDAARGOS_892	GCA_016027695.1	61645	61645	type	True	93.2647	1276	1552	95	below_threshold
Enterobacter chengduensis	strain=WCHECl-C4 = WCHECh050004	GCA_001984825.2	2494701	2494701	type	True	92.9091	1309	1552	95	below_threshold
Enterobacter sichuanensis	strain=WCHECL1597	GCA_002939185.1	2071710	2071710	type	True	92.0056	1238	1552	95	below_threshold
Enterobacter sichuanensis	strain=WCHECL1597	GCA_025002605.1	2071710	2071710	type	True	91.9726	1275	1552	95	below_threshold
Enterobacter kobei	strain=DSM 13645	GCA_001729765.1	208224	208224	type	True	90.7354	1284	1552	95	below_threshold
Enterobacter wuhouensis	strain=WCHEW120002	GCA_004331265.1	2529381	2529381	type	True	89.1859	1203	1552	95	below_threshold
Enterobacter cloacae	strain=DSM 30054	GCA_021469225.1	550	550	type	True	89.1629	1274	1552	95	below_threshold
Enterobacter hormaechei	strain=FDAARGOS 1433	GCA_019048245.1	158836	158836	suspected-type	True	88.4976	1209	1552	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:23:37,435] [INFO] DFAST Taxonomy check result was written to GCF_024390995.1_ASM2439099v1_genomic.fna/tc_result.tsv
[2024-01-24 14:23:37,439] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:23:37,439] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:23:37,439] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stge7515855-2ef1-43f3-8e82-1f314474c219/dqc_reference/checkm_data
[2024-01-24 14:23:37,444] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:23:37,508] [INFO] Task started: CheckM
[2024-01-24 14:23:37,508] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_024390995.1_ASM2439099v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_024390995.1_ASM2439099v1_genomic.fna/checkm_input GCF_024390995.1_ASM2439099v1_genomic.fna/checkm_result
[2024-01-24 14:24:20,337] [INFO] Task succeeded: CheckM
[2024-01-24 14:24:20,339] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 98.96%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:24:20,365] [INFO] ===== Completeness check finished =====
[2024-01-24 14:24:20,366] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:24:20,366] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_024390995.1_ASM2439099v1_genomic.fna/markers.fasta)
[2024-01-24 14:24:20,366] [INFO] Task started: Blastn
[2024-01-24 14:24:20,367] [INFO] Running command: blastn -query GCF_024390995.1_ASM2439099v1_genomic.fna/markers.fasta -db /var/lib/cwl/stge7515855-2ef1-43f3-8e82-1f314474c219/dqc_reference/reference_markers_gtdb.fasta -out GCF_024390995.1_ASM2439099v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:24:21,631] [INFO] Task succeeded: Blastn
[2024-01-24 14:24:21,635] [INFO] Selected 16 target genomes.
[2024-01-24 14:24:21,635] [INFO] Target genome list was writen to GCF_024390995.1_ASM2439099v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:24:21,654] [INFO] Task started: fastANI
[2024-01-24 14:24:21,655] [INFO] Running command: fastANI --query /var/lib/cwl/stg0a6cf6f1-3df4-4db6-a8e2-7abcef042b84/GCF_024390995.1_ASM2439099v1_genomic.fna.gz --refList GCF_024390995.1_ASM2439099v1_genomic.fna/target_genomes_gtdb.txt --output GCF_024390995.1_ASM2439099v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:24:39,435] [INFO] Task succeeded: fastANI
[2024-01-24 14:24:39,454] [INFO] Found 16 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:24:39,454] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001729805.1	s__Enterobacter roggenkampii	98.5003	1355	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.21	95.50	0.89	0.83	197	conclusive
GCF_009755685.1	s__Enterobacter sp000568095	94.3053	1286	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0476	98.37	98.04	0.95	0.92	11	-
GCF_008364555.1	s__Enterobacter vonholyi	94.2992	1283	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.34	98.09	0.94	0.93	8	-
GCF_008364625.1	s__Enterobacter dykesii	93.4851	1252	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	96.5795	99.13	97.97	0.97	0.95	7	-
GCF_003634515.1	s__Enterobacter asburiae_A	93.4011	1292	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	96.0274	97.62	96.46	0.91	0.85	7	-
GCF_007035645.1	s__Enterobacter asburiae_B	93.3884	1305	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	96.8818	98.31	97.34	0.92	0.85	70	-
GCF_001521715.1	s__Enterobacter asburiae	93.2701	1284	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	96.8818	98.42	96.92	0.89	0.84	32	-
GCF_000493015.1	s__Enterobacter sp000493015	93.2647	1286	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.7595	98.68	98.68	0.92	0.92	2	-
GCF_008080435.1	s__Enterobacter asburiae_C	93.0269	1281	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.5575	N/A	N/A	N/A	N/A	1	-
GCF_001984825.2	s__Enterobacter chengduensis	92.8966	1309	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	97.11	95.58	0.88	0.79	24	-
GCF_001022965.1	s__Enterobacter cloacae_L	92.6262	1288	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	97.51	97.16	0.91	0.90	5	-
GCF_002939185.1	s__Enterobacter sichuanensis	92.0056	1238	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.38	97.99	0.90	0.85	19	-
GCF_011754535.1	s__Enterobacter cloacae_N	91.7607	1274	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	99.03	98.99	0.93	0.91	4	-
GCF_001729765.1	s__Enterobacter kobei	90.7256	1286	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.81	98.45	0.89	0.85	141	-
GCF_900322725.1	s__Enterobacter quasihormaechei	88.4179	1210	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	99.46	97.61	0.96	0.89	41	-
GCF_013375935.1	s__Enterobacter cloacae_O	88.2603	1199	1552	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.68	98.59	0.94	0.92	6	-
--------------------------------------------------------------------------------
[2024-01-24 14:24:39,456] [INFO] GTDB search result was written to GCF_024390995.1_ASM2439099v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:24:39,456] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:24:39,460] [INFO] DFAST_QC result json was written to GCF_024390995.1_ASM2439099v1_genomic.fna/dqc_result.json
[2024-01-24 14:24:39,460] [INFO] DFAST_QC completed!
[2024-01-24 14:24:39,460] [INFO] Total running time: 0h1m34s