[2024-01-24 11:59:10,249] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:59:10,252] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:59:10,252] [INFO] DQC Reference Directory: /var/lib/cwl/stgeef1ca06-e338-4317-b345-ee3a85a38c1f/dqc_reference
[2024-01-24 11:59:11,542] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:59:11,543] [INFO] Task started: Prodigal
[2024-01-24 11:59:11,543] [INFO] Running command: gunzip -c /var/lib/cwl/stgd3530918-7e03-4980-9b56-a895580d23b6/GCF_024494685.1_ASM2449468v1_genomic.fna.gz | prodigal -d GCF_024494685.1_ASM2449468v1_genomic.fna/cds.fna -a GCF_024494685.1_ASM2449468v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:59:26,062] [INFO] Task succeeded: Prodigal
[2024-01-24 11:59:26,062] [INFO] Task started: HMMsearch
[2024-01-24 11:59:26,062] [INFO] Running command: hmmsearch --tblout GCF_024494685.1_ASM2449468v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgeef1ca06-e338-4317-b345-ee3a85a38c1f/dqc_reference/reference_markers.hmm GCF_024494685.1_ASM2449468v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:59:26,336] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:59:26,337] [WARNING] Found 3/6 markers. [/var/lib/cwl/stgd3530918-7e03-4980-9b56-a895580d23b6/GCF_024494685.1_ASM2449468v1_genomic.fna.gz]
[2024-01-24 11:59:26,364] [INFO] Query marker FASTA was written to GCF_024494685.1_ASM2449468v1_genomic.fna/markers.fasta
[2024-01-24 11:59:26,364] [INFO] Task started: Blastn
[2024-01-24 11:59:26,365] [INFO] Running command: blastn -query GCF_024494685.1_ASM2449468v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgeef1ca06-e338-4317-b345-ee3a85a38c1f/dqc_reference/reference_markers.fasta -out GCF_024494685.1_ASM2449468v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:59:26,901] [INFO] Task succeeded: Blastn
[2024-01-24 11:59:26,904] [INFO] Selected 6 target genomes.
[2024-01-24 11:59:26,904] [INFO] Target genome list was writen to GCF_024494685.1_ASM2449468v1_genomic.fna/target_genomes.txt
[2024-01-24 11:59:26,907] [INFO] Task started: fastANI
[2024-01-24 11:59:26,907] [INFO] Running command: fastANI --query /var/lib/cwl/stgd3530918-7e03-4980-9b56-a895580d23b6/GCF_024494685.1_ASM2449468v1_genomic.fna.gz --refList GCF_024494685.1_ASM2449468v1_genomic.fna/target_genomes.txt --output GCF_024494685.1_ASM2449468v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:59:33,514] [INFO] Task succeeded: fastANI
[2024-01-24 11:59:33,515] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgeef1ca06-e338-4317-b345-ee3a85a38c1f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:59:33,515] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgeef1ca06-e338-4317-b345-ee3a85a38c1f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:59:33,525] [INFO] Found 6 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 11:59:33,525] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:59:33,526] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Halorubrum rubrum	strain=YC87	GCA_024494685.1	1126240	1126240	type	True	100.0	1056	1063	95	conclusive
Halorubrum aethiopicum	strain=SAH-A6	GCA_001542905.1	1758255	1758255	type	True	92.2748	843	1063	95	below_threshold
Halorubrum halodurans	strain=Cb34	GCA_002252985.1	1383851	1383851	type	True	91.0625	738	1063	95	below_threshold
Halorubrum cibi	strain=DSM 19504	GCA_900182635.1	413815	413815	type	True	90.7543	805	1063	95	below_threshold
Halorubrum aquaticum	strain=CGMCC 1.6377	GCA_900113615.1	387340	387340	type	True	90.2103	796	1063	95	below_threshold
Halorubrum rutilum	strain=YJ-18-S1	GCA_024494565.1	1364933	1364933	type	True	83.5349	681	1063	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:59:33,527] [INFO] DFAST Taxonomy check result was written to GCF_024494685.1_ASM2449468v1_genomic.fna/tc_result.tsv
[2024-01-24 11:59:33,528] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:59:33,528] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:59:33,528] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgeef1ca06-e338-4317-b345-ee3a85a38c1f/dqc_reference/checkm_data
[2024-01-24 11:59:33,530] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:59:33,565] [INFO] Task started: CheckM
[2024-01-24 11:59:33,566] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_024494685.1_ASM2449468v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_024494685.1_ASM2449468v1_genomic.fna/checkm_input GCF_024494685.1_ASM2449468v1_genomic.fna/checkm_result
[2024-01-24 12:00:15,584] [INFO] Task succeeded: CheckM
[2024-01-24 12:00:15,585] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.67%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:00:15,607] [INFO] ===== Completeness check finished =====
[2024-01-24 12:00:15,607] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:00:15,608] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_024494685.1_ASM2449468v1_genomic.fna/markers.fasta)
[2024-01-24 12:00:15,608] [INFO] Task started: Blastn
[2024-01-24 12:00:15,609] [INFO] Running command: blastn -query GCF_024494685.1_ASM2449468v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgeef1ca06-e338-4317-b345-ee3a85a38c1f/dqc_reference/reference_markers_gtdb.fasta -out GCF_024494685.1_ASM2449468v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:00:16,159] [INFO] Task succeeded: Blastn
[2024-01-24 12:00:16,163] [INFO] Selected 6 target genomes.
[2024-01-24 12:00:16,163] [INFO] Target genome list was writen to GCF_024494685.1_ASM2449468v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:00:16,168] [INFO] Task started: fastANI
[2024-01-24 12:00:16,168] [INFO] Running command: fastANI --query /var/lib/cwl/stgd3530918-7e03-4980-9b56-a895580d23b6/GCF_024494685.1_ASM2449468v1_genomic.fna.gz --refList GCF_024494685.1_ASM2449468v1_genomic.fna/target_genomes_gtdb.txt --output GCF_024494685.1_ASM2449468v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:00:22,256] [INFO] Task succeeded: fastANI
[2024-01-24 12:00:22,262] [INFO] Found 6 fastANI hits (0 hits with ANI > circumscription radius)
[2024-01-24 12:00:22,262] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001542905.1	s__Halorubrum aethiopicum	92.2588	844	1063	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Halorubrum	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002252985.1	s__Halorubrum halodurans	91.0434	739	1063	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Halorubrum	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900182635.1	s__Halorubrum cibi	90.7694	803	1063	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Halorubrum	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900113615.1	s__Halorubrum aquaticum	90.2103	796	1063	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Halorubrum	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003697845.1	s__Halorubrum sp003697845	88.8287	843	1063	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Halorubrum	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003287355.1	s__Halorubrum sp003287355	88.4151	801	1063	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Halorubrum	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 12:00:22,263] [INFO] GTDB search result was written to GCF_024494685.1_ASM2449468v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:00:22,264] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:00:22,266] [INFO] DFAST_QC result json was written to GCF_024494685.1_ASM2449468v1_genomic.fna/dqc_result.json
[2024-01-24 12:00:22,266] [INFO] DFAST_QC completed!
[2024-01-24 12:00:22,267] [INFO] Total running time: 0h1m12s