[2024-01-24 11:36:20,675] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:36:20,678] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:36:20,678] [INFO] DQC Reference Directory: /var/lib/cwl/stgc070700c-b35c-4099-ad8a-1f96bc5b1a02/dqc_reference
[2024-01-24 11:36:21,863] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:36:21,863] [INFO] Task started: Prodigal
[2024-01-24 11:36:21,864] [INFO] Running command: gunzip -c /var/lib/cwl/stg35bd1d4f-b71c-4b9a-ad68-35277fc6028a/GCF_024494725.1_ASM2449472v1_genomic.fna.gz | prodigal -d GCF_024494725.1_ASM2449472v1_genomic.fna/cds.fna -a GCF_024494725.1_ASM2449472v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:36:38,473] [INFO] Task succeeded: Prodigal
[2024-01-24 11:36:38,474] [INFO] Task started: HMMsearch
[2024-01-24 11:36:38,474] [INFO] Running command: hmmsearch --tblout GCF_024494725.1_ASM2449472v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc070700c-b35c-4099-ad8a-1f96bc5b1a02/dqc_reference/reference_markers.hmm GCF_024494725.1_ASM2449472v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:36:38,687] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:36:38,689] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg35bd1d4f-b71c-4b9a-ad68-35277fc6028a/GCF_024494725.1_ASM2449472v1_genomic.fna.gz]
[2024-01-24 11:36:38,722] [INFO] Query marker FASTA was written to GCF_024494725.1_ASM2449472v1_genomic.fna/markers.fasta
[2024-01-24 11:36:38,722] [INFO] Task started: Blastn
[2024-01-24 11:36:38,722] [INFO] Running command: blastn -query GCF_024494725.1_ASM2449472v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc070700c-b35c-4099-ad8a-1f96bc5b1a02/dqc_reference/reference_markers.fasta -out GCF_024494725.1_ASM2449472v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:36:39,239] [INFO] Task succeeded: Blastn
[2024-01-24 11:36:39,242] [INFO] Selected 8 target genomes.
[2024-01-24 11:36:39,242] [INFO] Target genome list was writen to GCF_024494725.1_ASM2449472v1_genomic.fna/target_genomes.txt
[2024-01-24 11:36:39,246] [INFO] Task started: fastANI
[2024-01-24 11:36:39,246] [INFO] Running command: fastANI --query /var/lib/cwl/stg35bd1d4f-b71c-4b9a-ad68-35277fc6028a/GCF_024494725.1_ASM2449472v1_genomic.fna.gz --refList GCF_024494725.1_ASM2449472v1_genomic.fna/target_genomes.txt --output GCF_024494725.1_ASM2449472v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:36:45,489] [INFO] Task succeeded: fastANI
[2024-01-24 11:36:45,490] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc070700c-b35c-4099-ad8a-1f96bc5b1a02/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:36:45,491] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc070700c-b35c-4099-ad8a-1f96bc5b1a02/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:36:45,502] [INFO] Found 8 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 11:36:45,502] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:36:45,502] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Haloplanus ruber	strain=R35	GCA_024494725.1	869892	869892	type	True	100.0	1086	1086	95	conclusive
Haloplanus rubicundus	strain=CBA1112	GCA_003342675.1	1547898	1547898	type	True	83.1764	711	1086	95	below_threshold
Haloplanus natans	strain=DSM 17983	GCA_000427685.1	376171	376171	type	True	82.8315	680	1086	95	below_threshold
Haloplanus aerogenes	strain=CGMCC 1.10124	GCA_003688715.1	660522	660522	type	True	82.795	666	1086	95	below_threshold
Haloplanus aerogenes	strain=JCM 16430	GCA_003856835.1	660522	660522	type	True	82.7923	682	1086	95	below_threshold
Haloplanus salinus	strain=JCM 18368	GCA_003336245.1	1126245	1126245	type	True	82.5508	672	1086	95	below_threshold
Haloplanus vescus	strain=CGMCC 1.8712	GCA_900107665.1	555874	555874	type	True	82.314	591	1086	95	below_threshold
Halorussus halobius	strain=HD8-83	GCA_004765805.1	1710537	1710537	type	True	77.7093	327	1086	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:36:45,592] [INFO] DFAST Taxonomy check result was written to GCF_024494725.1_ASM2449472v1_genomic.fna/tc_result.tsv
[2024-01-24 11:36:45,593] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:36:45,593] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:36:45,593] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc070700c-b35c-4099-ad8a-1f96bc5b1a02/dqc_reference/checkm_data
[2024-01-24 11:36:45,596] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:36:45,632] [INFO] Task started: CheckM
[2024-01-24 11:36:45,632] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_024494725.1_ASM2449472v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_024494725.1_ASM2449472v1_genomic.fna/checkm_input GCF_024494725.1_ASM2449472v1_genomic.fna/checkm_result
[2024-01-24 11:37:32,672] [INFO] Task succeeded: CheckM
[2024-01-24 11:37:32,674] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:37:32,695] [INFO] ===== Completeness check finished =====
[2024-01-24 11:37:32,695] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:37:32,696] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_024494725.1_ASM2449472v1_genomic.fna/markers.fasta)
[2024-01-24 11:37:32,696] [INFO] Task started: Blastn
[2024-01-24 11:37:32,696] [INFO] Running command: blastn -query GCF_024494725.1_ASM2449472v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc070700c-b35c-4099-ad8a-1f96bc5b1a02/dqc_reference/reference_markers_gtdb.fasta -out GCF_024494725.1_ASM2449472v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:37:33,195] [INFO] Task succeeded: Blastn
[2024-01-24 11:37:33,200] [INFO] Selected 7 target genomes.
[2024-01-24 11:37:33,200] [INFO] Target genome list was writen to GCF_024494725.1_ASM2449472v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:37:33,206] [INFO] Task started: fastANI
[2024-01-24 11:37:33,207] [INFO] Running command: fastANI --query /var/lib/cwl/stg35bd1d4f-b71c-4b9a-ad68-35277fc6028a/GCF_024494725.1_ASM2449472v1_genomic.fna.gz --refList GCF_024494725.1_ASM2449472v1_genomic.fna/target_genomes_gtdb.txt --output GCF_024494725.1_ASM2449472v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:37:39,083] [INFO] Task succeeded: fastANI
[2024-01-24 11:37:39,097] [INFO] Found 7 fastANI hits (0 hits with ANI > circumscription radius)
[2024-01-24 11:37:39,097] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_009762275.1	s__Haloplanus rallus	88.5675	792	1086	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Haloplanus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003342675.1	s__Haloplanus rubicundus	83.1778	712	1086	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Haloplanus	95.0	98.71	98.71	0.88	0.88	2	-
GCF_000427685.1	s__Haloplanus natans	82.8209	681	1086	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Haloplanus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003856835.1	s__Haloplanus aerogenes	82.7844	684	1086	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Haloplanus	95.0	100.00	100.00	1.00	1.00	2	-
GCF_003336245.1	s__Haloplanus salinus	82.5186	675	1086	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Haloplanus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900107665.1	s__Haloplanus vescus	82.3128	594	1086	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haloferacaceae;g__Haloplanus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004765805.1	s__Halorussus sp004765805	77.6918	329	1086	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Halorussus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 11:37:39,099] [INFO] GTDB search result was written to GCF_024494725.1_ASM2449472v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:37:39,100] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:37:39,104] [INFO] DFAST_QC result json was written to GCF_024494725.1_ASM2449472v1_genomic.fna/dqc_result.json
[2024-01-24 11:37:39,104] [INFO] DFAST_QC completed!
[2024-01-24 11:37:39,105] [INFO] Total running time: 0h1m18s