[2024-01-24 11:18:17,701] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:18:17,703] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:18:17,703] [INFO] DQC Reference Directory: /var/lib/cwl/stga4c5438d-81cd-4027-93a1-bc9f486a73be/dqc_reference
[2024-01-24 11:18:19,086] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:18:19,087] [INFO] Task started: Prodigal
[2024-01-24 11:18:19,087] [INFO] Running command: gunzip -c /var/lib/cwl/stg46172f3c-e67a-402b-b544-6bb236cf272b/GCF_024505145.1_ASM2450514v1_genomic.fna.gz | prodigal -d GCF_024505145.1_ASM2450514v1_genomic.fna/cds.fna -a GCF_024505145.1_ASM2450514v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:18:56,337] [INFO] Task succeeded: Prodigal
[2024-01-24 11:18:56,338] [INFO] Task started: HMMsearch
[2024-01-24 11:18:56,338] [INFO] Running command: hmmsearch --tblout GCF_024505145.1_ASM2450514v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga4c5438d-81cd-4027-93a1-bc9f486a73be/dqc_reference/reference_markers.hmm GCF_024505145.1_ASM2450514v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:18:56,743] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:18:56,744] [INFO] Found 6/6 markers.
[2024-01-24 11:18:56,829] [INFO] Query marker FASTA was written to GCF_024505145.1_ASM2450514v1_genomic.fna/markers.fasta
[2024-01-24 11:18:56,829] [INFO] Task started: Blastn
[2024-01-24 11:18:56,829] [INFO] Running command: blastn -query GCF_024505145.1_ASM2450514v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga4c5438d-81cd-4027-93a1-bc9f486a73be/dqc_reference/reference_markers.fasta -out GCF_024505145.1_ASM2450514v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:18:58,130] [INFO] Task succeeded: Blastn
[2024-01-24 11:18:58,134] [INFO] Selected 16 target genomes.
[2024-01-24 11:18:58,135] [INFO] Target genome list was writen to GCF_024505145.1_ASM2450514v1_genomic.fna/target_genomes.txt
[2024-01-24 11:18:58,147] [INFO] Task started: fastANI
[2024-01-24 11:18:58,147] [INFO] Running command: fastANI --query /var/lib/cwl/stg46172f3c-e67a-402b-b544-6bb236cf272b/GCF_024505145.1_ASM2450514v1_genomic.fna.gz --refList GCF_024505145.1_ASM2450514v1_genomic.fna/target_genomes.txt --output GCF_024505145.1_ASM2450514v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:19:49,406] [INFO] Task succeeded: fastANI
[2024-01-24 11:19:49,407] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga4c5438d-81cd-4027-93a1-bc9f486a73be/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:19:49,408] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga4c5438d-81cd-4027-93a1-bc9f486a73be/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:19:49,425] [INFO] Found 16 fastANI hits (0 hits with ANI > threshold)
[2024-01-24 11:19:49,426] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-24 11:19:49,426] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Streptomyces iranensis	strain=DSM 41954	GCA_017874715.1	576784	576784	type	True	94.5221	2801	3563	95	below_threshold
Streptomyces rapamycinicus	strain=NRRL 5491	GCA_003675955.1	1226757	1226757	type	True	94.516	2911	3563	95	below_threshold
Streptomyces rapamycinicus	strain=NRRL 5491	GCA_024298965.1	1226757	1226757	type	True	94.4949	2910	3563	95	below_threshold
Streptomyces rapamycinicus	strain=DSM 41530	GCA_014205315.1	1226757	1226757	type	True	94.4497	2903	3563	95	below_threshold
Streptomyces rapamycinicus	strain=NRRL 5491	GCA_000418455.1	1226757	1226757	type	True	94.4466	2907	3563	95	below_threshold
Streptomyces javensis	strain=DSM 41764	GCA_016103505.1	114698	114698	type	True	94.4051	2208	3563	95	below_threshold
Streptomyces sabulosicollis	strain=PRKS01-29	GCA_016103465.1	2715963	2715963	type	True	93.8521	2244	3563	95	below_threshold
Streptomyces yatensis	strain=DSM 41771	GCA_018069625.1	155177	155177	type	True	92.1973	2760	3563	95	below_threshold
Streptomyces antimycoticus	strain=NBRC 12839	GCA_005405925.1	68175	68175	type	True	92.0212	2770	3563	95	below_threshold
Streptomyces hygroscopicus subsp. hygroscopicus	strain=NBRC 13472	GCA_001553455.1	68042	1912	type	True	90.4727	2114	3563	95	below_threshold
Streptomyces milbemycinicus	strain=NRRL 5739	GCA_002154605.1	476552	476552	type	True	84.2123	1855	3563	95	below_threshold
Streptomyces pactum	strain=ATCC 27456	GCA_016031615.1	68249	68249	type	True	81.6094	1512	3563	95	below_threshold
Streptomyces paromomycinus	strain=NBRC 15454	GCA_003865155.1	92743	92743	type	True	81.0517	1530	3563	95	below_threshold
Streptomyces albireticuli	strain=NRRL B1670	GCA_021228125.1	1940	1940	type	True	81.0236	1440	3563	95	below_threshold
Streptomyces rimosus subsp. rimosus	strain=R7	GCA_022760195.1	132474	1927	type	True	80.9585	1571	3563	95	below_threshold
Streptomyces monomycini	strain=NRRL B-24309	GCA_022271435.1	371720	371720	type	True	80.9097	1478	3563	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:19:49,428] [INFO] DFAST Taxonomy check result was written to GCF_024505145.1_ASM2450514v1_genomic.fna/tc_result.tsv
[2024-01-24 11:19:49,429] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:19:49,429] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:19:49,429] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga4c5438d-81cd-4027-93a1-bc9f486a73be/dqc_reference/checkm_data
[2024-01-24 11:19:49,430] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:19:49,527] [INFO] Task started: CheckM
[2024-01-24 11:19:49,527] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_024505145.1_ASM2450514v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_024505145.1_ASM2450514v1_genomic.fna/checkm_input GCF_024505145.1_ASM2450514v1_genomic.fna/checkm_result
[2024-01-24 11:22:07,166] [INFO] Task succeeded: CheckM
[2024-01-24 11:22:07,168] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 5.21%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:22:07,193] [INFO] ===== Completeness check finished =====
[2024-01-24 11:22:07,194] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:22:07,194] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_024505145.1_ASM2450514v1_genomic.fna/markers.fasta)
[2024-01-24 11:22:07,194] [INFO] Task started: Blastn
[2024-01-24 11:22:07,195] [INFO] Running command: blastn -query GCF_024505145.1_ASM2450514v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga4c5438d-81cd-4027-93a1-bc9f486a73be/dqc_reference/reference_markers_gtdb.fasta -out GCF_024505145.1_ASM2450514v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:22:09,266] [INFO] Task succeeded: Blastn
[2024-01-24 11:22:09,270] [INFO] Selected 10 target genomes.
[2024-01-24 11:22:09,271] [INFO] Target genome list was writen to GCF_024505145.1_ASM2450514v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:22:09,457] [INFO] Task started: fastANI
[2024-01-24 11:22:09,457] [INFO] Running command: fastANI --query /var/lib/cwl/stg46172f3c-e67a-402b-b544-6bb236cf272b/GCF_024505145.1_ASM2450514v1_genomic.fna.gz --refList GCF_024505145.1_ASM2450514v1_genomic.fna/target_genomes_gtdb.txt --output GCF_024505145.1_ASM2450514v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:22:49,318] [INFO] Task succeeded: fastANI
[2024-01-24 11:22:49,331] [INFO] Found 10 fastANI hits (0 hits with ANI > circumscription radius)
[2024-01-24 11:22:49,332] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_016741935.1	s__Streptomyces sp016741935	94.8838	3073	3563	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0488	N/A	N/A	N/A	N/A	1	-
GCF_018138715.1	s__Streptomyces asiaticus	94.6776	2903	3563	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	98.17	95.36	0.90	0.81	10	-
GCF_017874715.1	s__Streptomyces iranensis	94.5742	2794	3563	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.67	99.98	99.98	1.00	1.00	2	-
GCA_005405945.1	s__Streptomyces violaceusniger	94.5255	2829	3563	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	97.67	97.67	0.85	0.85	2	-
GCF_003675955.1	s__Streptomyces rapamycinicus	94.5193	2910	3563	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.67	99.95	99.91	0.99	0.99	3	-
GCF_000968685.2	s__Streptomyces antioxidans	93.4413	2363	3563	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCF_015689475.1	s__Streptomyces solisilvae	92.2055	2781	3563	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	98.71	97.94	0.94	0.86	10	-
GCF_018069625.1	s__Streptomyces yatensis	92.1944	2760	3563	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	96.0185	N/A	N/A	N/A	N/A	1	-
GCF_000147815.2	s__Streptomyces violaceusniger_A	91.984	2708	3563	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.3301	95.69	95.54	0.80	0.80	4	-
GCF_001553455.1	s__Streptomyces hygroscopicus	90.4642	2115	3563	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	98.24	98.00	0.92	0.90	7	-
--------------------------------------------------------------------------------
[2024-01-24 11:22:49,333] [INFO] GTDB search result was written to GCF_024505145.1_ASM2450514v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:22:49,334] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:22:49,337] [INFO] DFAST_QC result json was written to GCF_024505145.1_ASM2450514v1_genomic.fna/dqc_result.json
[2024-01-24 11:22:49,338] [INFO] DFAST_QC completed!
[2024-01-24 11:22:49,338] [INFO] Total running time: 0h4m32s