[2024-01-24 11:05:25,360] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:05:25,363] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:05:25,364] [INFO] DQC Reference Directory: /var/lib/cwl/stgc08a09e2-ec9b-481a-9503-627bc81e8065/dqc_reference
[2024-01-24 11:05:26,839] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:05:26,840] [INFO] Task started: Prodigal
[2024-01-24 11:05:26,840] [INFO] Running command: gunzip -c /var/lib/cwl/stgce13d856-66a4-4301-aa4a-24efd1185752/GCF_024508335.1_ASM2450833v1_genomic.fna.gz | prodigal -d GCF_024508335.1_ASM2450833v1_genomic.fna/cds.fna -a GCF_024508335.1_ASM2450833v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:05:37,239] [INFO] Task succeeded: Prodigal
[2024-01-24 11:05:37,239] [INFO] Task started: HMMsearch
[2024-01-24 11:05:37,239] [INFO] Running command: hmmsearch --tblout GCF_024508335.1_ASM2450833v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc08a09e2-ec9b-481a-9503-627bc81e8065/dqc_reference/reference_markers.hmm GCF_024508335.1_ASM2450833v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:05:37,511] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:05:37,512] [INFO] Found 6/6 markers.
[2024-01-24 11:05:37,553] [INFO] Query marker FASTA was written to GCF_024508335.1_ASM2450833v1_genomic.fna/markers.fasta
[2024-01-24 11:05:37,554] [INFO] Task started: Blastn
[2024-01-24 11:05:37,554] [INFO] Running command: blastn -query GCF_024508335.1_ASM2450833v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc08a09e2-ec9b-481a-9503-627bc81e8065/dqc_reference/reference_markers.fasta -out GCF_024508335.1_ASM2450833v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:05:38,841] [INFO] Task succeeded: Blastn
[2024-01-24 11:05:38,844] [INFO] Selected 22 target genomes.
[2024-01-24 11:05:38,845] [INFO] Target genome list was writen to GCF_024508335.1_ASM2450833v1_genomic.fna/target_genomes.txt
[2024-01-24 11:05:38,854] [INFO] Task started: fastANI
[2024-01-24 11:05:38,854] [INFO] Running command: fastANI --query /var/lib/cwl/stgce13d856-66a4-4301-aa4a-24efd1185752/GCF_024508335.1_ASM2450833v1_genomic.fna.gz --refList GCF_024508335.1_ASM2450833v1_genomic.fna/target_genomes.txt --output GCF_024508335.1_ASM2450833v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:05:56,814] [INFO] Task succeeded: fastANI
[2024-01-24 11:05:56,815] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc08a09e2-ec9b-481a-9503-627bc81e8065/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:05:56,815] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc08a09e2-ec9b-481a-9503-627bc81e8065/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:05:56,840] [INFO] Found 22 fastANI hits (0 hits with ANI > threshold)
[2024-01-24 11:05:56,840] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-24 11:05:56,840] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Cellulomonas timonensis	strain=SN7	GCA_900046455.1	1689271	1689271	type	True	87.003	991	1190	95	below_threshold
Cellulomonas cellasea	strain=NBRC 3753	GCA_006538745.1	43670	43670	type	True	81.0982	708	1190	95	below_threshold
Cellulomonas cellasea	strain=DSM 20118	GCA_000767135.1	43670	43670	type	True	81.0974	570	1190	95	below_threshold
Cellulomonas biazotea	strain=NBRC12680	GCA_004306155.1	1709	1709	type	True	80.4761	606	1190	95	below_threshold
Cellulomonas hominis	strain=DSM 9581	GCA_014201095.1	156981	156981	suspected-type	True	80.3976	658	1190	95	below_threshold
Cellulomonas terrae	strain=NBRC 100819	GCA_007990265.1	311234	311234	type	True	80.3436	629	1190	95	below_threshold
Cellulomonas soli	strain=DSM 24484	GCA_013409305.1	931535	931535	type	True	80.3092	626	1190	95	below_threshold
Cellulomonas hominis	strain=NBRC 16055	GCA_007989225.1	156981	156981	suspected-type	True	80.2993	633	1190	95	below_threshold
Cellulomonas palmilytica	strain=EW123	GCA_021590045.1	2608402	2608402	type	True	80.282	608	1190	95	below_threshold
Cellulomonas xylanilytica	strain=NBRC 101102	GCA_007989805.1	233583	233583	type	True	80.1642	645	1190	95	below_threshold
Cellulomonas oligotrophica	strain=DSM 24482	GCA_013409875.1	931536	931536	type	True	80.0938	642	1190	95	below_threshold
Cellulomonas fulva	strain=DKR-3	GCA_018531375.1	2835530	2835530	type	True	80.0814	588	1190	95	below_threshold
Cellulomonas telluris	strain=CPCC 204705	GCA_004886325.1	2306636	2306636	type	True	80.0786	618	1190	95	below_threshold
Cellulomonas avistercoris	strain=Sa3CUA2	GCA_014836445.1	2762242	2762242	type	True	79.9421	595	1190	95	below_threshold
Oerskovia jenensis	strain=DSM 46000	GCA_016907235.1	162169	162169	type	True	79.4928	507	1190	95	below_threshold
Oerskovia turbata	strain=JCM3160	GCA_004121315.1	1713	1713	type	True	79.4477	488	1190	95	below_threshold
Actinotalea solisilvae	strain=KACC 19191	GCA_016464425.1	2072922	2072922	type	True	79.4327	571	1190	95	below_threshold
Oerskovia turbata	strain=NRRL B-8019	GCA_000718325.1	1713	1713	type	True	79.3707	484	1190	95	below_threshold
Oerskovia merdavium	strain=Sa2CUA9	GCA_014836755.1	2762227	2762227	type	True	79.2916	498	1190	95	below_threshold
Sanguibacter keddieii	strain=DSM 10542	GCA_000024925.1	60920	60920	type	True	79.1706	518	1190	95	below_threshold
Cellulomonas carbonis	strain=T26	GCA_000767175.1	1386092	1386092	type	True	79.1385	495	1190	95	below_threshold
Sanguibacter suarezii	strain=NBRC 16159	GCA_001552735.1	60921	60921	type	True	79.0606	459	1190	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:05:56,842] [INFO] DFAST Taxonomy check result was written to GCF_024508335.1_ASM2450833v1_genomic.fna/tc_result.tsv
[2024-01-24 11:05:56,843] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:05:56,843] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:05:56,843] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc08a09e2-ec9b-481a-9503-627bc81e8065/dqc_reference/checkm_data
[2024-01-24 11:05:56,845] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:05:56,885] [INFO] Task started: CheckM
[2024-01-24 11:05:56,885] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_024508335.1_ASM2450833v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_024508335.1_ASM2450833v1_genomic.fna/checkm_input GCF_024508335.1_ASM2450833v1_genomic.fna/checkm_result
[2024-01-24 11:07:01,169] [INFO] Task succeeded: CheckM
[2024-01-24 11:07:01,170] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:07:01,191] [INFO] ===== Completeness check finished =====
[2024-01-24 11:07:01,191] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:07:01,192] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_024508335.1_ASM2450833v1_genomic.fna/markers.fasta)
[2024-01-24 11:07:01,192] [INFO] Task started: Blastn
[2024-01-24 11:07:01,192] [INFO] Running command: blastn -query GCF_024508335.1_ASM2450833v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc08a09e2-ec9b-481a-9503-627bc81e8065/dqc_reference/reference_markers_gtdb.fasta -out GCF_024508335.1_ASM2450833v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:07:03,275] [INFO] Task succeeded: Blastn
[2024-01-24 11:07:03,279] [INFO] Selected 23 target genomes.
[2024-01-24 11:07:03,280] [INFO] Target genome list was writen to GCF_024508335.1_ASM2450833v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:07:03,308] [INFO] Task started: fastANI
[2024-01-24 11:07:03,308] [INFO] Running command: fastANI --query /var/lib/cwl/stgce13d856-66a4-4301-aa4a-24efd1185752/GCF_024508335.1_ASM2450833v1_genomic.fna.gz --refList GCF_024508335.1_ASM2450833v1_genomic.fna/target_genomes_gtdb.txt --output GCF_024508335.1_ASM2450833v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:07:22,107] [INFO] Task succeeded: fastANI
[2024-01-24 11:07:22,137] [INFO] Found 23 fastANI hits (0 hits with ANI > circumscription radius)
[2024-01-24 11:07:22,137] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_900046455.1	s__Cellulomonas timonensis	87.006	990	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	100.00	100.00	0.99	0.99	2	-
GCF_006538745.1	s__Cellulomonas cellasea	81.0151	717	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	97.79	95.59	0.95	0.91	3	-
GCF_003115775.1	s__Cellulomonas sp003115775	80.8573	594	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_013409475.1	s__Cellulomonas hominis_B	80.4815	648	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_008033115.1	s__Cellulomonas sp008033115	80.4762	667	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	96.34	96.34	0.91	0.91	2	-
GCF_005239125.1	s__Cellulomonas hominis_A	80.4665	583	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_011603975.1	s__Cellulomonas sp011603975	80.4249	568	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_901521055.1	s__Cellulomonas hominis_C	80.3713	632	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003470205.1	s__Cellulomonas rhizosphaerae	80.3522	546	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001426705.1	s__Cellulomonas sp001426705	80.3503	632	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_007990265.1	s__Cellulomonas terrae	80.34	629	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016862675.1	s__Cellulomonas chitinilytica	80.2667	655	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_014763765.1	s__Cellulomonas sp014763765	80.2248	565	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016862755.1	s__Cellulomonas pakistanensis	80.2089	652	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_007679345.1	s__Cellulomonas cellulans_A	80.194	663	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018388465.1	s__Cellulomonas sp018388465	80.1326	647	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	99.65	99.29	0.98	0.97	3	-
GCF_001424125.1	s__Cellulomonas sp001424125	80.1247	599	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017744555.1	s__Cellulomonas sp017744555	80.0632	623	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_006386485.1	s__Cellulomonas oligotrophica	79.997	628	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	100.00	100.00	1.00	1.00	3	-
GCF_001722485.1	s__Cellulosimicrobium cellulans_A	79.4148	465	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulosimicrobium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003052455.1	s__Cellulosimicrobium cinnabarinum	79.1683	539	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulosimicrobium	96.0261	97.94	97.40	0.92	0.90	4	-
GCF_001552735.1	s__Sanguibacter suarezii	79.0612	459	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Sanguibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009708005.2	s__Cellulosimicrobium sp009708005	78.9416	553	1190	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulosimicrobium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 11:07:22,139] [INFO] GTDB search result was written to GCF_024508335.1_ASM2450833v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:07:22,139] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:07:22,144] [INFO] DFAST_QC result json was written to GCF_024508335.1_ASM2450833v1_genomic.fna/dqc_result.json
[2024-01-24 11:07:22,144] [INFO] DFAST_QC completed!
[2024-01-24 11:07:22,144] [INFO] Total running time: 0h1m57s