[2024-01-24 10:48:09,562] [INFO] DFAST_QC pipeline started.
[2024-01-24 10:48:09,565] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 10:48:09,565] [INFO] DQC Reference Directory: /var/lib/cwl/stg7922f474-8d62-456f-98e1-29801155061a/dqc_reference
[2024-01-24 10:48:23,956] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 10:48:23,958] [INFO] Task started: Prodigal
[2024-01-24 10:48:23,959] [INFO] Running command: gunzip -c /var/lib/cwl/stg6697a9f3-855c-4fcd-b9ef-d1c4baedfd20/GCF_025002605.1_ASM2500260v1_genomic.fna.gz | prodigal -d GCF_025002605.1_ASM2500260v1_genomic.fna/cds.fna -a GCF_025002605.1_ASM2500260v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 10:48:37,507] [INFO] Task succeeded: Prodigal
[2024-01-24 10:48:37,507] [INFO] Task started: HMMsearch
[2024-01-24 10:48:37,507] [INFO] Running command: hmmsearch --tblout GCF_025002605.1_ASM2500260v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg7922f474-8d62-456f-98e1-29801155061a/dqc_reference/reference_markers.hmm GCF_025002605.1_ASM2500260v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 10:48:37,811] [INFO] Task succeeded: HMMsearch
[2024-01-24 10:48:37,813] [INFO] Found 6/6 markers.
[2024-01-24 10:48:37,858] [INFO] Query marker FASTA was written to GCF_025002605.1_ASM2500260v1_genomic.fna/markers.fasta
[2024-01-24 10:48:37,858] [INFO] Task started: Blastn
[2024-01-24 10:48:37,858] [INFO] Running command: blastn -query GCF_025002605.1_ASM2500260v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg7922f474-8d62-456f-98e1-29801155061a/dqc_reference/reference_markers.fasta -out GCF_025002605.1_ASM2500260v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 10:48:38,692] [INFO] Task succeeded: Blastn
[2024-01-24 10:48:38,695] [INFO] Selected 18 target genomes.
[2024-01-24 10:48:38,696] [INFO] Target genome list was writen to GCF_025002605.1_ASM2500260v1_genomic.fna/target_genomes.txt
[2024-01-24 10:48:38,704] [INFO] Task started: fastANI
[2024-01-24 10:48:38,704] [INFO] Running command: fastANI --query /var/lib/cwl/stg6697a9f3-855c-4fcd-b9ef-d1c4baedfd20/GCF_025002605.1_ASM2500260v1_genomic.fna.gz --refList GCF_025002605.1_ASM2500260v1_genomic.fna/target_genomes.txt --output GCF_025002605.1_ASM2500260v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 10:49:00,304] [INFO] Task succeeded: fastANI
[2024-01-24 10:49:00,304] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg7922f474-8d62-456f-98e1-29801155061a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 10:49:00,305] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg7922f474-8d62-456f-98e1-29801155061a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 10:49:00,330] [INFO] Found 18 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 10:49:00,330] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 10:49:00,331] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Enterobacter sichuanensis	strain=WCHECL1597	GCA_025002605.1	2071710	2071710	type	True	100.0	1642	1642	95	conclusive
Enterobacter sichuanensis	strain=WCHECL1597	GCA_002939185.1	2071710	2071710	type	True	98.1069	1392	1642	95	conclusive
Enterobacter roggenkampii	strain=DSM 16690	GCA_024390995.1	1812935	1812935	type	True	91.8832	1285	1642	95	below_threshold
Enterobacter roggenkampii	strain=FDAARGOS 1430	GCA_019047025.1	1812935	1812935	type	True	91.8807	1338	1642	95	below_threshold
Enterobacter roggenkampii	strain=DSM 16690	GCA_001729805.1	1812935	1812935	type	True	91.8656	1333	1642	95	below_threshold
Enterobacter asburiae	strain=ATCC 35953	GCA_001521715.1	61645	61645	type	True	91.7422	1289	1642	95	below_threshold
Enterobacter asburiae	strain=FDAARGOS_892	GCA_016027695.1	61645	61645	type	True	91.6595	1292	1642	95	below_threshold
Enterobacter chengduensis	strain=WCHECl-C4 = WCHECh050004	GCA_001984825.2	2494701	2494701	type	True	90.9883	1315	1642	95	below_threshold
Enterobacter bugandensis		GCA_900324475.1	881260	881260	type	True	90.317	1317	1642	95	below_threshold
Enterobacter bugandensis	strain=FDAARGOS 1427	GCA_019046905.1	881260	881260	type	True	90.295	1314	1642	95	below_threshold
Enterobacter cloacae	strain=ATCC 13047	GCA_000755525.1	550	550	type	True	89.3927	1360	1642	95	below_threshold
Enterobacter cloacae	strain=DSM 30054	GCA_013376815.1	550	550	type	True	89.3702	1350	1642	95	below_threshold
Enterobacter cloacae	strain=FDAARGOS 1431	GCA_019047105.1	550	550	type	True	89.3642	1374	1642	95	below_threshold
Enterobacter quasimori	strain=090044	GCA_003964905.1	2838947	2838947	type	True	89.2897	1305	1642	95	below_threshold
Enterobacter cloacae	strain=DSM 30054	GCA_021469225.1	550	550	type	True	89.2871	1323	1642	95	below_threshold
Enterobacter mori	strain=LMG 25706	GCA_000211415.1	539813	539813	type	True	88.9586	1273	1642	95	below_threshold
Enterobacter wuhouensis	strain=WCHEW120002	GCA_004331265.1	2529381	2529381	type	True	88.2701	1234	1642	95	below_threshold
Enterobacter hormaechei	strain=FDAARGOS 1433	GCA_019048245.1	158836	158836	suspected-type	True	87.5298	1217	1642	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 10:49:00,332] [INFO] DFAST Taxonomy check result was written to GCF_025002605.1_ASM2500260v1_genomic.fna/tc_result.tsv
[2024-01-24 10:49:00,333] [INFO] ===== Taxonomy check completed =====
[2024-01-24 10:49:00,333] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 10:49:00,333] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg7922f474-8d62-456f-98e1-29801155061a/dqc_reference/checkm_data
[2024-01-24 10:49:00,335] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 10:49:00,404] [INFO] Task started: CheckM
[2024-01-24 10:49:00,404] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_025002605.1_ASM2500260v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_025002605.1_ASM2500260v1_genomic.fna/checkm_input GCF_025002605.1_ASM2500260v1_genomic.fna/checkm_result
[2024-01-24 10:49:46,388] [INFO] Task succeeded: CheckM
[2024-01-24 10:49:46,389] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 98.96%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 10:49:46,412] [INFO] ===== Completeness check finished =====
[2024-01-24 10:49:46,412] [INFO] ===== Start GTDB Search =====
[2024-01-24 10:49:46,413] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_025002605.1_ASM2500260v1_genomic.fna/markers.fasta)
[2024-01-24 10:49:46,413] [INFO] Task started: Blastn
[2024-01-24 10:49:46,413] [INFO] Running command: blastn -query GCF_025002605.1_ASM2500260v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg7922f474-8d62-456f-98e1-29801155061a/dqc_reference/reference_markers_gtdb.fasta -out GCF_025002605.1_ASM2500260v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 10:49:47,645] [INFO] Task succeeded: Blastn
[2024-01-24 10:49:47,651] [INFO] Selected 23 target genomes.
[2024-01-24 10:49:47,651] [INFO] Target genome list was writen to GCF_025002605.1_ASM2500260v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 10:49:47,694] [INFO] Task started: fastANI
[2024-01-24 10:49:47,695] [INFO] Running command: fastANI --query /var/lib/cwl/stg6697a9f3-855c-4fcd-b9ef-d1c4baedfd20/GCF_025002605.1_ASM2500260v1_genomic.fna.gz --refList GCF_025002605.1_ASM2500260v1_genomic.fna/target_genomes_gtdb.txt --output GCF_025002605.1_ASM2500260v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 10:50:12,181] [INFO] Task succeeded: fastANI
[2024-01-24 10:50:12,203] [INFO] Found 21 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 10:50:12,204] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002939185.1	s__Enterobacter sichuanensis	98.1069	1392	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.38	97.99	0.90	0.85	19	conclusive
GCF_001022965.1	s__Enterobacter cloacae_L	92.4813	1401	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	97.51	97.16	0.91	0.90	5	-
GCF_001729805.1	s__Enterobacter roggenkampii	91.8656	1333	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.21	95.50	0.89	0.83	197	-
GCF_009755685.1	s__Enterobacter sp000568095	91.861	1347	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0476	98.37	98.04	0.95	0.92	11	-
GCF_001521715.1	s__Enterobacter asburiae	91.7422	1289	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	96.8818	98.42	96.92	0.89	0.84	32	-
GCF_003634515.1	s__Enterobacter asburiae_A	91.695	1340	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	96.0274	97.62	96.46	0.91	0.85	7	-
GCF_007035645.1	s__Enterobacter asburiae_B	91.6059	1334	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	96.8818	98.31	97.34	0.92	0.85	70	-
GCF_000493015.1	s__Enterobacter sp000493015	91.5788	1326	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.7595	98.68	98.68	0.92	0.92	2	-
GCF_008364625.1	s__Enterobacter dykesii	91.5178	1302	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	96.5795	99.13	97.97	0.97	0.95	7	-
GCF_008364555.1	s__Enterobacter vonholyi	91.4997	1305	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.34	98.09	0.94	0.93	8	-
GCF_002208095.1	s__Enterobacter cloacae_M	91.4435	1341	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	96.7528	98.28	96.89	0.91	0.85	84	-
GCF_001984825.2	s__Enterobacter chengduensis	90.9883	1315	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	97.11	95.58	0.88	0.79	24	-
GCF_011754535.1	s__Enterobacter cloacae_N	90.4528	1335	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	99.03	98.99	0.93	0.91	4	-
GCF_003594915.1	s__Enterobacter chuandaensis	90.3234	1339	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.38	98.32	0.92	0.92	3	-
GCF_900324475.1	s__Enterobacter bugandensis	90.317	1317	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.49	95.52	0.94	0.88	115	-
GCF_001729765.1	s__Enterobacter kobei	89.9075	1268	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.81	98.45	0.89	0.85	141	-
GCF_003046225.1	s__Enterobacter cloacae_I	89.8958	1306	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	97.61	95.47	0.89	0.86	14	-
GCF_000025565.1	s__Enterobacter cloacae	89.2921	1386	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Enterobacter	95.0	98.05	95.14	0.91	0.80	129	-
GCF_018323885.1	s__Citrobacter_A amalonaticus	81.8534	923	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Citrobacter_A	95.0	98.67	95.02	0.91	0.83	59	-
GCF_001266615.1	s__Kluyvera cryocrescens_A	81.2842	821	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Kluyvera	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001756855.1	s__Erwinia dacicola	79.3801	241	1642	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Erwinia	95.0	98.47	98.24	0.66	0.61	4	-
--------------------------------------------------------------------------------
[2024-01-24 10:50:12,206] [INFO] GTDB search result was written to GCF_025002605.1_ASM2500260v1_genomic.fna/result_gtdb.tsv
[2024-01-24 10:50:12,207] [INFO] ===== GTDB Search completed =====
[2024-01-24 10:50:12,213] [INFO] DFAST_QC result json was written to GCF_025002605.1_ASM2500260v1_genomic.fna/dqc_result.json
[2024-01-24 10:50:12,214] [INFO] DFAST_QC completed!
[2024-01-24 10:50:12,214] [INFO] Total running time: 0h2m3s