[2024-01-24 12:43:54,223] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:43:54,225] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:43:54,225] [INFO] DQC Reference Directory: /var/lib/cwl/stg2ccff751-9efa-455e-8052-027d8b1682a2/dqc_reference
[2024-01-24 12:43:59,335] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:43:59,336] [INFO] Task started: Prodigal
[2024-01-24 12:43:59,336] [INFO] Running command: gunzip -c /var/lib/cwl/stg3da5bcc8-4d46-4d49-a77f-372e4bcc5df5/GCF_025148125.1_ASM2514812v1_genomic.fna.gz | prodigal -d GCF_025148125.1_ASM2514812v1_genomic.fna/cds.fna -a GCF_025148125.1_ASM2514812v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:44:10,534] [INFO] Task succeeded: Prodigal
[2024-01-24 12:44:10,534] [INFO] Task started: HMMsearch
[2024-01-24 12:44:10,534] [INFO] Running command: hmmsearch --tblout GCF_025148125.1_ASM2514812v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg2ccff751-9efa-455e-8052-027d8b1682a2/dqc_reference/reference_markers.hmm GCF_025148125.1_ASM2514812v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:44:10,834] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:44:10,835] [INFO] Found 6/6 markers.
[2024-01-24 12:44:10,876] [INFO] Query marker FASTA was written to GCF_025148125.1_ASM2514812v1_genomic.fna/markers.fasta
[2024-01-24 12:44:10,877] [INFO] Task started: Blastn
[2024-01-24 12:44:10,877] [INFO] Running command: blastn -query GCF_025148125.1_ASM2514812v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2ccff751-9efa-455e-8052-027d8b1682a2/dqc_reference/reference_markers.fasta -out GCF_025148125.1_ASM2514812v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:44:11,520] [INFO] Task succeeded: Blastn
[2024-01-24 12:44:11,523] [INFO] Selected 13 target genomes.
[2024-01-24 12:44:11,523] [INFO] Target genome list was writen to GCF_025148125.1_ASM2514812v1_genomic.fna/target_genomes.txt
[2024-01-24 12:44:11,549] [INFO] Task started: fastANI
[2024-01-24 12:44:11,549] [INFO] Running command: fastANI --query /var/lib/cwl/stg3da5bcc8-4d46-4d49-a77f-372e4bcc5df5/GCF_025148125.1_ASM2514812v1_genomic.fna.gz --refList GCF_025148125.1_ASM2514812v1_genomic.fna/target_genomes.txt --output GCF_025148125.1_ASM2514812v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:44:22,071] [INFO] Task succeeded: fastANI
[2024-01-24 12:44:22,072] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg2ccff751-9efa-455e-8052-027d8b1682a2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:44:22,073] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg2ccff751-9efa-455e-8052-027d8b1682a2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:44:22,087] [INFO] Found 12 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 12:44:22,087] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:44:22,087] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Blautia wexlerae	strain=DSM 19850	GCA_025148125.1	418240	418240	suspected-type	True	100.0	1561	1561	95	conclusive
Blautia wexlerae	strain=DSM 19850	GCA_000484655.1	418240	418240	suspected-type	True	99.8514	1386	1561	95	conclusive
Blautia luti	strain=DSM 14534	GCA_009707925.1	89014	89014	suspected-type	True	86.6259	742	1561	95	below_threshold
Eubacterium ventriosum	strain=ATCC 27560	GCA_025150745.1	39496	39496	suspected-type	True	83.6533	89	1561	95	below_threshold
Blautia massiliensis	strain=GD9	GCA_001487165.1	1737424	1737424	type	True	83.0584	444	1561	95	below_threshold
Mediterraneibacter butyricigenes	strain=KCTC 15684	GCA_003574295.1	2316025	2316025	type	True	82.911	162	1561	95	below_threshold
Blautia caecimuris	strain=DSM 29492	GCA_024622975.1	1796615	1796615	type	True	82.2201	359	1561	95	below_threshold
Blautia obeum	strain=ATCC 29174	GCA_025147765.1	40520	40520	type	True	81.8655	390	1561	95	below_threshold
Blautia obeum	strain=ATCC 29174	GCA_000153905.1	40520	40520	type	True	81.4931	388	1561	95	below_threshold
Blautia intestinalis	strain=27-44	GCA_014297355.1	2763028	2763028	type	True	80.5326	351	1561	95	below_threshold
Jingyaoa shaoxingensis	strain=NSJ-46	GCA_014385005.1	2763671	2763671	type	True	80.0951	212	1561	95	below_threshold
Roseburia faecis	strain=M72	GCA_001406815.1	301302	301302	type	True	79.0234	109	1561	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:44:22,089] [INFO] DFAST Taxonomy check result was written to GCF_025148125.1_ASM2514812v1_genomic.fna/tc_result.tsv
[2024-01-24 12:44:22,089] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:44:22,089] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:44:22,090] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg2ccff751-9efa-455e-8052-027d8b1682a2/dqc_reference/checkm_data
[2024-01-24 12:44:22,091] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:44:22,135] [INFO] Task started: CheckM
[2024-01-24 12:44:22,135] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_025148125.1_ASM2514812v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_025148125.1_ASM2514812v1_genomic.fna/checkm_input GCF_025148125.1_ASM2514812v1_genomic.fna/checkm_result
[2024-01-24 12:44:59,828] [INFO] Task succeeded: CheckM
[2024-01-24 12:44:59,829] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:44:59,847] [INFO] ===== Completeness check finished =====
[2024-01-24 12:44:59,847] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:44:59,847] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_025148125.1_ASM2514812v1_genomic.fna/markers.fasta)
[2024-01-24 12:44:59,848] [INFO] Task started: Blastn
[2024-01-24 12:44:59,848] [INFO] Running command: blastn -query GCF_025148125.1_ASM2514812v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2ccff751-9efa-455e-8052-027d8b1682a2/dqc_reference/reference_markers_gtdb.fasta -out GCF_025148125.1_ASM2514812v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:45:00,864] [INFO] Task succeeded: Blastn
[2024-01-24 12:45:00,868] [INFO] Selected 7 target genomes.
[2024-01-24 12:45:00,868] [INFO] Target genome list was writen to GCF_025148125.1_ASM2514812v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:45:00,883] [INFO] Task started: fastANI
[2024-01-24 12:45:00,883] [INFO] Running command: fastANI --query /var/lib/cwl/stg3da5bcc8-4d46-4d49-a77f-372e4bcc5df5/GCF_025148125.1_ASM2514812v1_genomic.fna.gz --refList GCF_025148125.1_ASM2514812v1_genomic.fna/target_genomes_gtdb.txt --output GCF_025148125.1_ASM2514812v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:45:08,882] [INFO] Task succeeded: fastANI
[2024-01-24 12:45:08,892] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:45:08,893] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000484655.1	s__Blautia_A wexlerae	99.8514	1386	1561	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Blautia_A	95.0	97.38	96.15	0.76	0.65	140	conclusive
GCF_013300825.1	s__Blautia_A wexlerae_B	94.2969	914	1561	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Blautia_A	95.0	98.68	98.07	0.89	0.86	5	-
GCF_003471165.1	s__Blautia_A sp003471165	88.439	913	1561	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Blautia_A	95.0	98.40	97.74	0.83	0.79	20	-
GCF_018919065.1	s__Blautia_A sp018919065	88.0267	732	1561	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Blautia_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009707925.1	s__Blautia_A luti	86.6617	741	1561	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Blautia_A	95.0	98.33	97.04	0.87	0.84	5	-
GCF_003477525.1	s__Blautia_A sp003477525	86.311	808	1561	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Blautia_A	95.0	99.30	98.60	0.90	0.81	3	-
GCA_905209435.1	s__Blautia_A sp905209435	85.2747	624	1561	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__Blautia_A	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 12:45:08,899] [INFO] GTDB search result was written to GCF_025148125.1_ASM2514812v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:45:08,900] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:45:08,908] [INFO] DFAST_QC result json was written to GCF_025148125.1_ASM2514812v1_genomic.fna/dqc_result.json
[2024-01-24 12:45:08,908] [INFO] DFAST_QC completed!
[2024-01-24 12:45:08,908] [INFO] Total running time: 0h1m15s