[2024-01-24 11:51:06,028] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:51:06,030] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:51:06,031] [INFO] DQC Reference Directory: /var/lib/cwl/stgc629a3ce-46af-4528-b01b-1f6ed685df12/dqc_reference
[2024-01-24 11:51:07,309] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:51:07,310] [INFO] Task started: Prodigal
[2024-01-24 11:51:07,311] [INFO] Running command: gunzip -c /var/lib/cwl/stg1bc0a909-c67c-4626-bd60-6f4c9a224306/GCF_025994955.1_ASM2599495v1_genomic.fna.gz | prodigal -d GCF_025994955.1_ASM2599495v1_genomic.fna/cds.fna -a GCF_025994955.1_ASM2599495v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:51:16,089] [INFO] Task succeeded: Prodigal
[2024-01-24 11:51:16,090] [INFO] Task started: HMMsearch
[2024-01-24 11:51:16,090] [INFO] Running command: hmmsearch --tblout GCF_025994955.1_ASM2599495v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc629a3ce-46af-4528-b01b-1f6ed685df12/dqc_reference/reference_markers.hmm GCF_025994955.1_ASM2599495v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:51:16,382] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:51:16,383] [INFO] Found 6/6 markers.
[2024-01-24 11:51:16,411] [INFO] Query marker FASTA was written to GCF_025994955.1_ASM2599495v1_genomic.fna/markers.fasta
[2024-01-24 11:51:16,412] [INFO] Task started: Blastn
[2024-01-24 11:51:16,412] [INFO] Running command: blastn -query GCF_025994955.1_ASM2599495v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc629a3ce-46af-4528-b01b-1f6ed685df12/dqc_reference/reference_markers.fasta -out GCF_025994955.1_ASM2599495v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:51:17,177] [INFO] Task succeeded: Blastn
[2024-01-24 11:51:17,180] [INFO] Selected 14 target genomes.
[2024-01-24 11:51:17,180] [INFO] Target genome list was writen to GCF_025994955.1_ASM2599495v1_genomic.fna/target_genomes.txt
[2024-01-24 11:51:17,185] [INFO] Task started: fastANI
[2024-01-24 11:51:17,185] [INFO] Running command: fastANI --query /var/lib/cwl/stg1bc0a909-c67c-4626-bd60-6f4c9a224306/GCF_025994955.1_ASM2599495v1_genomic.fna.gz --refList GCF_025994955.1_ASM2599495v1_genomic.fna/target_genomes.txt --output GCF_025994955.1_ASM2599495v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:51:25,790] [INFO] Task succeeded: fastANI
[2024-01-24 11:51:25,790] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc629a3ce-46af-4528-b01b-1f6ed685df12/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:51:25,791] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc629a3ce-46af-4528-b01b-1f6ed685df12/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:51:25,807] [INFO] Found 13 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 11:51:25,807] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:51:25,807] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Gluconobacter japonicus	strain=NBRC 3271	GCA_002723975.1	376620	376620	type	True	99.9953	981	1000	95	conclusive
Gluconobacter frateurii	strain=NBRC 3264	GCA_002723955.1	38308	38308	suspected-type	True	88.0354	765	1000	95	below_threshold
Gluconobacter thailandicus	strain=NBRC 100600	GCA_007990185.1	257438	257438	type	True	86.9223	793	1000	95	below_threshold
Gluconobacter thailandicus	strain=F149-1	GCA_000964465.1	257438	257438	type	True	86.8767	783	1000	95	below_threshold
Gluconobacter cerinus	strain=NBRC 3267	GCA_002723935.1	38307	38307	type	True	83.0532	609	1000	95	below_threshold
Gluconobacter aidae	strain=AC10	GCA_009547075.1	2662454	2662454	type	True	80.0149	393	1000	95	below_threshold
Gluconobacter potus	strain=R-71646	GCA_015244705.1	2724927	2724927	type	True	79.977	410	1000	95	below_threshold
Gluconobacter cerevisiae	strain=LMG 27748	GCA_015244675.1	1379734	1379734	type	True	79.7107	401	1000	95	below_threshold
Gluconobacter cerevisiae	strain=DSM 27644	GCA_018811965.1	1379734	1379734	type	True	79.6898	394	1000	95	below_threshold
Gluconobacter kanchanaburiensis	strain=NBRC 103587	GCA_007988905.1	563199	563199	type	True	79.5835	358	1000	95	below_threshold
Gluconobacter morbifer	strain=G707	GCA_000234355.2	479935	479935	type	True	79.1596	360	1000	95	below_threshold
Neokomagataea anthophila	strain=TBRC 2177	GCA_018122595.1	2826925	2826925	type	True	77.5519	129	1000	95	below_threshold
Acetobacter garciniae	strain=TBRC 12339	GCA_017377735.1	2817435	2817435	type	True	76.8392	88	1000	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:51:25,809] [INFO] DFAST Taxonomy check result was written to GCF_025994955.1_ASM2599495v1_genomic.fna/tc_result.tsv
[2024-01-24 11:51:25,810] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:51:25,810] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:51:25,810] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc629a3ce-46af-4528-b01b-1f6ed685df12/dqc_reference/checkm_data
[2024-01-24 11:51:25,811] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:51:25,851] [INFO] Task started: CheckM
[2024-01-24 11:51:25,852] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_025994955.1_ASM2599495v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_025994955.1_ASM2599495v1_genomic.fna/checkm_input GCF_025994955.1_ASM2599495v1_genomic.fna/checkm_result
[2024-01-24 11:51:57,665] [INFO] Task succeeded: CheckM
[2024-01-24 11:51:57,666] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:51:57,689] [INFO] ===== Completeness check finished =====
[2024-01-24 11:51:57,690] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:51:57,690] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_025994955.1_ASM2599495v1_genomic.fna/markers.fasta)
[2024-01-24 11:51:57,690] [INFO] Task started: Blastn
[2024-01-24 11:51:57,691] [INFO] Running command: blastn -query GCF_025994955.1_ASM2599495v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc629a3ce-46af-4528-b01b-1f6ed685df12/dqc_reference/reference_markers_gtdb.fasta -out GCF_025994955.1_ASM2599495v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:51:58,959] [INFO] Task succeeded: Blastn
[2024-01-24 11:51:58,963] [INFO] Selected 11 target genomes.
[2024-01-24 11:51:58,963] [INFO] Target genome list was writen to GCF_025994955.1_ASM2599495v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:51:58,972] [INFO] Task started: fastANI
[2024-01-24 11:51:58,972] [INFO] Running command: fastANI --query /var/lib/cwl/stg1bc0a909-c67c-4626-bd60-6f4c9a224306/GCF_025994955.1_ASM2599495v1_genomic.fna.gz --refList GCF_025994955.1_ASM2599495v1_genomic.fna/target_genomes_gtdb.txt --output GCF_025994955.1_ASM2599495v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:52:06,047] [INFO] Task succeeded: fastANI
[2024-01-24 11:52:06,067] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:52:06,067] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002723975.1	s__Gluconobacter_A japonicus	99.9953	981	1000	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter_A	95.0	96.84	95.27	0.89	0.85	9	conclusive
GCF_002723955.1	s__Gluconobacter_A frateurii	88.0354	765	1000	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter_A	95.0	97.06	97.06	0.93	0.93	2	-
GCF_000964465.1	s__Gluconobacter_A thailandicus	86.9023	781	1000	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter_A	95.0	98.77	97.43	0.93	0.88	11	-
GCF_014132155.1	s__Gluconobacter_A sp014132155	83.1151	622	1000	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter_A	95.0	97.46	96.68	0.90	0.89	4	-
GCF_002723935.1	s__Gluconobacter_A cerinus	83.0462	610	1000	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter_A	95.0	96.40	95.61	0.88	0.86	20	-
GCF_001580675.1	s__Gluconobacter potus	80.1409	405	1000	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	96.92	96.89	0.82	0.80	7	-
GCF_009547075.1	s__Gluconobacter sp009547075	80.0149	393	1000	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_015244675.1	s__Gluconobacter cerevisiae	79.7107	401	1000	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	99.99	99.99	0.99	0.99	2	-
GCF_006538885.1	s__Gluconobacter roseus	79.6338	407	1000	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	99.99	99.99	1.00	1.00	2	-
GCF_007988905.1	s__Gluconobacter kanchanaburiensis	79.5835	358	1000	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	100.00	100.00	1.00	1.00	2	-
GCF_000234355.1	s__Gluconobacter_C morbifer	79.1797	359	1000	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter_C	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 11:52:06,069] [INFO] GTDB search result was written to GCF_025994955.1_ASM2599495v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:52:06,071] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:52:06,074] [INFO] DFAST_QC result json was written to GCF_025994955.1_ASM2599495v1_genomic.fna/dqc_result.json
[2024-01-24 11:52:06,074] [INFO] DFAST_QC completed!
[2024-01-24 11:52:06,074] [INFO] Total running time: 0h1m0s