[2024-01-24 14:47:05,220] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:47:05,225] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:47:05,225] [INFO] DQC Reference Directory: /var/lib/cwl/stga2f92923-9bf3-476c-9b3a-0c6f7125db33/dqc_reference
[2024-01-24 14:47:06,475] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:47:06,476] [INFO] Task started: Prodigal
[2024-01-24 14:47:06,476] [INFO] Running command: gunzip -c /var/lib/cwl/stg9572affb-8d2c-4ecb-b169-5ab8230908ad/GCF_025995575.1_ASM2599557v1_genomic.fna.gz | prodigal -d GCF_025995575.1_ASM2599557v1_genomic.fna/cds.fna -a GCF_025995575.1_ASM2599557v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:47:14,935] [INFO] Task succeeded: Prodigal
[2024-01-24 14:47:14,936] [INFO] Task started: HMMsearch
[2024-01-24 14:47:14,936] [INFO] Running command: hmmsearch --tblout GCF_025995575.1_ASM2599557v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga2f92923-9bf3-476c-9b3a-0c6f7125db33/dqc_reference/reference_markers.hmm GCF_025995575.1_ASM2599557v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:47:15,180] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:47:15,182] [INFO] Found 6/6 markers.
[2024-01-24 14:47:15,212] [INFO] Query marker FASTA was written to GCF_025995575.1_ASM2599557v1_genomic.fna/markers.fasta
[2024-01-24 14:47:15,213] [INFO] Task started: Blastn
[2024-01-24 14:47:15,213] [INFO] Running command: blastn -query GCF_025995575.1_ASM2599557v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga2f92923-9bf3-476c-9b3a-0c6f7125db33/dqc_reference/reference_markers.fasta -out GCF_025995575.1_ASM2599557v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:47:15,893] [INFO] Task succeeded: Blastn
[2024-01-24 14:47:15,897] [INFO] Selected 9 target genomes.
[2024-01-24 14:47:15,897] [INFO] Target genome list was writen to GCF_025995575.1_ASM2599557v1_genomic.fna/target_genomes.txt
[2024-01-24 14:47:15,902] [INFO] Task started: fastANI
[2024-01-24 14:47:15,902] [INFO] Running command: fastANI --query /var/lib/cwl/stg9572affb-8d2c-4ecb-b169-5ab8230908ad/GCF_025995575.1_ASM2599557v1_genomic.fna.gz --refList GCF_025995575.1_ASM2599557v1_genomic.fna/target_genomes.txt --output GCF_025995575.1_ASM2599557v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:47:21,884] [INFO] Task succeeded: fastANI
[2024-01-24 14:47:21,884] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga2f92923-9bf3-476c-9b3a-0c6f7125db33/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:47:21,885] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga2f92923-9bf3-476c-9b3a-0c6f7125db33/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:47:21,893] [INFO] Found 9 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 14:47:21,893] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:47:21,893] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Acetobacter orientalis	strain=NBRC 16606	GCA_007989335.1	146474	146474	type	True	99.9903	936	949	95	conclusive
Acetobacter orientalis	strain=21F-2	GCA_000963965.1	146474	146474	type	True	99.9811	932	949	95	conclusive
Acetobacter cibinongensis	strain=NBRC 16605	GCA_007989305.1	146475	146475	type	True	80.0304	434	949	95	below_threshold
Acetobacter cibinongensis	strain=4H-1	GCA_000963925.1	146475	146475	type	True	79.9833	434	949	95	below_threshold
Acetobacter indonesiensis	strain=NBRC 16471	GCA_007991075.1	104101	104101	type	True	79.3803	334	949	95	below_threshold
Acetobacter indonesiensis	strain=5H-1	GCA_000963945.1	104101	104101	type	True	79.3043	342	949	95	below_threshold
Acetobacter senegalensis	strain=LMG 23690	GCA_001580995.1	446692	446692	type	True	79.1886	346	949	95	below_threshold
Acetobacter tropicalis	strain=NBRC 16470	GCA_007989245.1	104102	104102	type	True	78.8559	320	949	95	below_threshold
Acetobacter ascendens	strain=LMG 1590	GCA_001766235.1	481146	481146	type	True	77.9508	249	949	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:47:21,895] [INFO] DFAST Taxonomy check result was written to GCF_025995575.1_ASM2599557v1_genomic.fna/tc_result.tsv
[2024-01-24 14:47:21,895] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:47:21,896] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:47:21,896] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga2f92923-9bf3-476c-9b3a-0c6f7125db33/dqc_reference/checkm_data
[2024-01-24 14:47:21,897] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:47:21,930] [INFO] Task started: CheckM
[2024-01-24 14:47:21,930] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_025995575.1_ASM2599557v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_025995575.1_ASM2599557v1_genomic.fna/checkm_input GCF_025995575.1_ASM2599557v1_genomic.fna/checkm_result
[2024-01-24 14:47:55,389] [INFO] Task succeeded: CheckM
[2024-01-24 14:47:55,391] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:47:55,408] [INFO] ===== Completeness check finished =====
[2024-01-24 14:47:55,408] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:47:55,409] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_025995575.1_ASM2599557v1_genomic.fna/markers.fasta)
[2024-01-24 14:47:55,409] [INFO] Task started: Blastn
[2024-01-24 14:47:55,410] [INFO] Running command: blastn -query GCF_025995575.1_ASM2599557v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga2f92923-9bf3-476c-9b3a-0c6f7125db33/dqc_reference/reference_markers_gtdb.fasta -out GCF_025995575.1_ASM2599557v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:47:56,517] [INFO] Task succeeded: Blastn
[2024-01-24 14:47:56,520] [INFO] Selected 14 target genomes.
[2024-01-24 14:47:56,520] [INFO] Target genome list was writen to GCF_025995575.1_ASM2599557v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:47:56,539] [INFO] Task started: fastANI
[2024-01-24 14:47:56,540] [INFO] Running command: fastANI --query /var/lib/cwl/stg9572affb-8d2c-4ecb-b169-5ab8230908ad/GCF_025995575.1_ASM2599557v1_genomic.fna.gz --refList GCF_025995575.1_ASM2599557v1_genomic.fna/target_genomes_gtdb.txt --output GCF_025995575.1_ASM2599557v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:48:05,282] [INFO] Task succeeded: fastANI
[2024-01-24 14:48:05,294] [INFO] Found 13 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:48:05,295] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000963965.1	s__Acetobacter orientalis	99.9811	932	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	98.55	97.80	0.92	0.88	7	conclusive
GCF_000963925.1	s__Acetobacter cibinongensis	79.9833	434	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	98.47	96.95	0.93	0.86	3	-
GCF_000963945.1	s__Acetobacter indonesiensis	79.3043	342	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	98.28	96.85	0.92	0.88	4	-
GCF_001580995.1	s__Acetobacter senegalensis	79.1886	346	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	97.25	96.97	0.85	0.80	6	-
GCF_001580915.1	s__Acetobacter tropicalis	78.931	321	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	98.32	97.71	0.90	0.87	9	-
GCF_000613905.1	s__Acetobacter persici	78.8414	259	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	96.00	95.85	0.80	0.79	8	-
GCF_001580535.1	s__Acetobacter cerevisiae	78.6066	279	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	96.52	96.47	0.88	0.86	4	-
GCF_001580615.1	s__Acetobacter malorum	78.5312	280	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	95.98	95.83	0.82	0.80	5	-
GCF_000723785.2	s__Acetobacter pasteurianus	78.2297	256	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	98.11	97.27	0.89	0.78	27	-
GCF_004014775.2	s__Acetobacter oryzoeni	78.1343	272	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	97.72	97.29	0.89	0.84	4	-
GCF_002738225.1	s__Acetobacter pomorum	78.0596	240	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	97.71	97.71	0.83	0.83	2	-
GCF_001766235.1	s__Acetobacter ascendens	77.9515	249	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	98.38	98.13	0.85	0.83	6	-
GCF_001499675.1	s__Acetobacter ghanensis	77.8807	238	949	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Acetobacter	95.0	99.99	99.99	1.00	1.00	2	-
--------------------------------------------------------------------------------
[2024-01-24 14:48:05,296] [INFO] GTDB search result was written to GCF_025995575.1_ASM2599557v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:48:05,297] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:48:05,302] [INFO] DFAST_QC result json was written to GCF_025995575.1_ASM2599557v1_genomic.fna/dqc_result.json
[2024-01-24 14:48:05,302] [INFO] DFAST_QC completed!
[2024-01-24 14:48:05,302] [INFO] Total running time: 0h1m0s