[2024-01-24 11:26:03,034] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:26:03,035] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:26:03,035] [INFO] DQC Reference Directory: /var/lib/cwl/stg91ea2a56-4f9a-498b-8a8e-a34cd9a04dd2/dqc_reference
[2024-01-24 11:26:04,195] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:26:04,196] [INFO] Task started: Prodigal
[2024-01-24 11:26:04,196] [INFO] Running command: gunzip -c /var/lib/cwl/stgf527e065-c558-4c7a-afa7-b5d72a31d89a/GCF_026015395.1_ASM2601539v1_genomic.fna.gz | prodigal -d GCF_026015395.1_ASM2601539v1_genomic.fna/cds.fna -a GCF_026015395.1_ASM2601539v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:26:21,542] [INFO] Task succeeded: Prodigal
[2024-01-24 11:26:21,542] [INFO] Task started: HMMsearch
[2024-01-24 11:26:21,542] [INFO] Running command: hmmsearch --tblout GCF_026015395.1_ASM2601539v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg91ea2a56-4f9a-498b-8a8e-a34cd9a04dd2/dqc_reference/reference_markers.hmm GCF_026015395.1_ASM2601539v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:26:21,739] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:26:21,740] [INFO] Found 6/6 markers.
[2024-01-24 11:26:21,766] [INFO] Query marker FASTA was written to GCF_026015395.1_ASM2601539v1_genomic.fna/markers.fasta
[2024-01-24 11:26:21,766] [INFO] Task started: Blastn
[2024-01-24 11:26:21,766] [INFO] Running command: blastn -query GCF_026015395.1_ASM2601539v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg91ea2a56-4f9a-498b-8a8e-a34cd9a04dd2/dqc_reference/reference_markers.fasta -out GCF_026015395.1_ASM2601539v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:26:22,342] [INFO] Task succeeded: Blastn
[2024-01-24 11:26:22,346] [INFO] Selected 15 target genomes.
[2024-01-24 11:26:22,346] [INFO] Target genome list was writen to GCF_026015395.1_ASM2601539v1_genomic.fna/target_genomes.txt
[2024-01-24 11:26:22,353] [INFO] Task started: fastANI
[2024-01-24 11:26:22,353] [INFO] Running command: fastANI --query /var/lib/cwl/stgf527e065-c558-4c7a-afa7-b5d72a31d89a/GCF_026015395.1_ASM2601539v1_genomic.fna.gz --refList GCF_026015395.1_ASM2601539v1_genomic.fna/target_genomes.txt --output GCF_026015395.1_ASM2601539v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:26:34,078] [INFO] Task succeeded: fastANI
[2024-01-24 11:26:34,079] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg91ea2a56-4f9a-498b-8a8e-a34cd9a04dd2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:26:34,079] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg91ea2a56-4f9a-498b-8a8e-a34cd9a04dd2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:26:34,089] [INFO] Found 12 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 11:26:34,089] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:26:34,089] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Prevotella copri	strain=DSM 18205	GCA_025151535.1	165179	165179	suspected-type	True	99.9757	1106	1163	95	conclusive
Prevotella copri	strain=DSM 18205	GCA_000157935.1	165179	165179	suspected-type	True	99.9423	1130	1163	95	conclusive
Prevotella copri	strain=DSM 18205	GCA_009495405.1	165179	165179	suspected-type	True	99.8657	1050	1163	95	conclusive
Prevotella hominis	strain=BCRC 81118	GCA_004535825.1	2518605	2518605	type	True	84.068	554	1163	95	below_threshold
Prevotella fusca	strain=W1435	GCA_018128145.1	589436	589436	type	True	78.8674	123	1163	95	below_threshold
Prevotella fusca	strain=W1435	GCA_001262015.1	589436	589436	type	True	78.8497	124	1163	95	below_threshold
Prevotella bryantii	strain=B14	GCA_022024235.1	77095	77095	type	True	78.7981	129	1163	95	below_threshold
Prevotella scopos	strain=W2052	GCA_001683355.1	589437	589437	type	True	78.6145	97	1163	95	below_threshold
Prevotella stercorea	strain=DSM 18206	GCA_000235885.1	363265	363265	suspected-type	True	78.6074	144	1163	95	below_threshold
Prevotella vespertina	strain=A2879	GCA_009728485.1	2608404	2608404	type	True	78.4574	77	1163	95	below_threshold
Prevotella illustrans	strain=A2931	GCA_017426725.1	2800387	2800387	type	True	77.6737	76	1163	95	below_threshold
Phocaeicola plebeius	strain=DSM 17135	GCA_000187895.1	310297	310297	suspected-type	True	77.5795	56	1163	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:26:34,091] [INFO] DFAST Taxonomy check result was written to GCF_026015395.1_ASM2601539v1_genomic.fna/tc_result.tsv
[2024-01-24 11:26:34,091] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:26:34,091] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:26:34,091] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg91ea2a56-4f9a-498b-8a8e-a34cd9a04dd2/dqc_reference/checkm_data
[2024-01-24 11:26:34,092] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:26:34,125] [INFO] Task started: CheckM
[2024-01-24 11:26:34,125] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_026015395.1_ASM2601539v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_026015395.1_ASM2601539v1_genomic.fna/checkm_input GCF_026015395.1_ASM2601539v1_genomic.fna/checkm_result
[2024-01-24 11:27:25,051] [INFO] Task succeeded: CheckM
[2024-01-24 11:27:25,052] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:27:25,068] [INFO] ===== Completeness check finished =====
[2024-01-24 11:27:25,068] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:27:25,068] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_026015395.1_ASM2601539v1_genomic.fna/markers.fasta)
[2024-01-24 11:27:25,068] [INFO] Task started: Blastn
[2024-01-24 11:27:25,068] [INFO] Running command: blastn -query GCF_026015395.1_ASM2601539v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg91ea2a56-4f9a-498b-8a8e-a34cd9a04dd2/dqc_reference/reference_markers_gtdb.fasta -out GCF_026015395.1_ASM2601539v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:27:25,912] [INFO] Task succeeded: Blastn
[2024-01-24 11:27:25,915] [INFO] Selected 10 target genomes.
[2024-01-24 11:27:25,915] [INFO] Target genome list was writen to GCF_026015395.1_ASM2601539v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:27:25,929] [INFO] Task started: fastANI
[2024-01-24 11:27:25,930] [INFO] Running command: fastANI --query /var/lib/cwl/stgf527e065-c558-4c7a-afa7-b5d72a31d89a/GCF_026015395.1_ASM2601539v1_genomic.fna.gz --refList GCF_026015395.1_ASM2601539v1_genomic.fna/target_genomes_gtdb.txt --output GCF_026015395.1_ASM2601539v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:27:35,220] [INFO] Task succeeded: fastANI
[2024-01-24 11:27:35,229] [INFO] Found 10 fastANI hits (2 hits with ANI > circumscription radius)
[2024-01-24 11:27:35,229] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000157935.1	s__Prevotella copri	99.9443	1129	1163	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.6733	96.34	95.85	0.75	0.69	97	inconclusive
GCF_015074785.1	s__Prevotella sp015074785	95.737	857	1163	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.6733	96.50	95.88	0.80	0.71	45	inconclusive
GCF_019127135.1	s__Prevotella sp900551275	94.5058	823	1163	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0063	98.12	96.96	0.86	0.77	7	-
GCA_900557255.1	s__Prevotella sp900557255	93.945	485	1163	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009494395.1	s__Prevotella sp900546535	87.2658	712	1163	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	96.78	96.11	0.82	0.79	8	-
GCA_019249655.1	s__Prevotella sp900767615	86.7174	710	1163	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	96.47	95.77	0.82	0.75	7	-
GCF_002224675.1	s__Prevotella copri_A	86.6946	691	1163	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	96.28	96.07	0.78	0.74	13	-
GCA_900544825.1	s__Prevotella sp900544825	86.078	637	1163	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	99.36	98.76	0.90	0.84	3	-
GCA_900551985.1	s__Prevotella sp900551985	85.5854	563	1163	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	99.28	99.28	0.87	0.87	2	-
GCA_900765465.1	s__Prevotella sp900765465	82.8096	319	1163	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Prevotella	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 11:27:35,231] [INFO] GTDB search result was written to GCF_026015395.1_ASM2601539v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:27:35,231] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:27:35,234] [INFO] DFAST_QC result json was written to GCF_026015395.1_ASM2601539v1_genomic.fna/dqc_result.json
[2024-01-24 11:27:35,234] [INFO] DFAST_QC completed!
[2024-01-24 11:27:35,234] [INFO] Total running time: 0h1m32s