[2024-01-24 11:20:19,592] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:20:19,594] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:20:19,594] [INFO] DQC Reference Directory: /var/lib/cwl/stgb17acb3e-39a3-4c1c-a1e0-2418dac5fd7c/dqc_reference
[2024-01-24 11:20:20,840] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:20:20,840] [INFO] Task started: Prodigal
[2024-01-24 11:20:20,841] [INFO] Running command: gunzip -c /var/lib/cwl/stgfc3181f9-b323-4a40-a0de-ef5897b63b14/GCF_030160195.1_ASM3016019v1_genomic.fna.gz | prodigal -d GCF_030160195.1_ASM3016019v1_genomic.fna/cds.fna -a GCF_030160195.1_ASM3016019v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:20:30,125] [INFO] Task succeeded: Prodigal
[2024-01-24 11:20:30,126] [INFO] Task started: HMMsearch
[2024-01-24 11:20:30,126] [INFO] Running command: hmmsearch --tblout GCF_030160195.1_ASM3016019v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb17acb3e-39a3-4c1c-a1e0-2418dac5fd7c/dqc_reference/reference_markers.hmm GCF_030160195.1_ASM3016019v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:20:30,421] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:20:30,422] [INFO] Found 6/6 markers.
[2024-01-24 11:20:30,455] [INFO] Query marker FASTA was written to GCF_030160195.1_ASM3016019v1_genomic.fna/markers.fasta
[2024-01-24 11:20:30,455] [INFO] Task started: Blastn
[2024-01-24 11:20:30,455] [INFO] Running command: blastn -query GCF_030160195.1_ASM3016019v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb17acb3e-39a3-4c1c-a1e0-2418dac5fd7c/dqc_reference/reference_markers.fasta -out GCF_030160195.1_ASM3016019v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:20:31,142] [INFO] Task succeeded: Blastn
[2024-01-24 11:20:31,145] [INFO] Selected 12 target genomes.
[2024-01-24 11:20:31,145] [INFO] Target genome list was writen to GCF_030160195.1_ASM3016019v1_genomic.fna/target_genomes.txt
[2024-01-24 11:20:31,162] [INFO] Task started: fastANI
[2024-01-24 11:20:31,163] [INFO] Running command: fastANI --query /var/lib/cwl/stgfc3181f9-b323-4a40-a0de-ef5897b63b14/GCF_030160195.1_ASM3016019v1_genomic.fna.gz --refList GCF_030160195.1_ASM3016019v1_genomic.fna/target_genomes.txt --output GCF_030160195.1_ASM3016019v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:20:39,223] [INFO] Task succeeded: fastANI
[2024-01-24 11:20:39,223] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb17acb3e-39a3-4c1c-a1e0-2418dac5fd7c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:20:39,224] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb17acb3e-39a3-4c1c-a1e0-2418dac5fd7c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:20:39,240] [INFO] Found 10 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 11:20:39,240] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:20:39,240] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Gluconobacter albidus	strain=NBRC 3250	GCA_002723915.1	318683	318683	type	True	99.9915	1062	1075	95	conclusive
Gluconobacter cadivus	strain=LMG 1744	GCA_015244745.1	2728101	2728101	type	True	91.0641	882	1075	95	below_threshold
Gluconobacter kondonii	strain=NBRC 3266	GCA_002723995.1	941463	941463	type	True	90.6608	836	1075	95	below_threshold
Gluconobacter cerevisiae	strain=DSM 27644	GCA_018811965.1	1379734	1379734	type	True	89.5955	800	1075	95	below_threshold
Gluconobacter cerevisiae	strain=LMG 27748	GCA_015244675.1	1379734	1379734	type	True	89.5813	791	1075	95	below_threshold
Gluconobacter aidae	strain=AC10	GCA_009547075.1	2662454	2662454	type	True	86.9651	725	1075	95	below_threshold
Gluconobacter sphaericus	strain=LMG1414	GCA_015244605.1	574987	574987	type	True	86.5416	715	1075	95	below_threshold
Gluconobacter sphaericus	strain=NBRC 12467	GCA_006539125.1	574987	574987	type	True	86.4561	717	1075	95	below_threshold
Gluconobacter potus	strain=R-71646	GCA_015244705.1	2724927	2724927	type	True	86.3548	755	1075	95	below_threshold
Asaia lannensis	strain=NBRC 102526	GCA_024054035.1	415421	415421	type	True	77.9483	166	1075	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:20:39,242] [INFO] DFAST Taxonomy check result was written to GCF_030160195.1_ASM3016019v1_genomic.fna/tc_result.tsv
[2024-01-24 11:20:39,243] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:20:39,243] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:20:39,243] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb17acb3e-39a3-4c1c-a1e0-2418dac5fd7c/dqc_reference/checkm_data
[2024-01-24 11:20:39,244] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:20:39,276] [INFO] Task started: CheckM
[2024-01-24 11:20:39,276] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_030160195.1_ASM3016019v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_030160195.1_ASM3016019v1_genomic.fna/checkm_input GCF_030160195.1_ASM3016019v1_genomic.fna/checkm_result
[2024-01-24 11:21:11,927] [INFO] Task succeeded: CheckM
[2024-01-24 11:21:11,928] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:21:11,948] [INFO] ===== Completeness check finished =====
[2024-01-24 11:21:11,948] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:21:11,949] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_030160195.1_ASM3016019v1_genomic.fna/markers.fasta)
[2024-01-24 11:21:11,949] [INFO] Task started: Blastn
[2024-01-24 11:21:11,949] [INFO] Running command: blastn -query GCF_030160195.1_ASM3016019v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb17acb3e-39a3-4c1c-a1e0-2418dac5fd7c/dqc_reference/reference_markers_gtdb.fasta -out GCF_030160195.1_ASM3016019v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:21:12,919] [INFO] Task succeeded: Blastn
[2024-01-24 11:21:12,924] [INFO] Selected 10 target genomes.
[2024-01-24 11:21:12,924] [INFO] Target genome list was writen to GCF_030160195.1_ASM3016019v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:21:12,926] [INFO] Task started: fastANI
[2024-01-24 11:21:12,927] [INFO] Running command: fastANI --query /var/lib/cwl/stgfc3181f9-b323-4a40-a0de-ef5897b63b14/GCF_030160195.1_ASM3016019v1_genomic.fna.gz --refList GCF_030160195.1_ASM3016019v1_genomic.fna/target_genomes_gtdb.txt --output GCF_030160195.1_ASM3016019v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:21:19,928] [INFO] Task succeeded: fastANI
[2024-01-24 11:21:19,937] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:21:19,937] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002723915.1	s__Gluconobacter albidus	99.9915	1062	1075	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	98.35	97.61	0.92	0.87	6	conclusive
GCF_015244745.1	s__Gluconobacter cadivus	91.0782	881	1075	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	96.53	96.31	0.92	0.90	5	-
GCF_002723995.1	s__Gluconobacter kondonii	90.6758	836	1075	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	97.23	97.04	0.89	0.87	8	-
GCF_015244675.1	s__Gluconobacter cerevisiae	89.5813	791	1075	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	99.99	99.99	0.99	0.99	2	-
GCF_009547075.1	s__Gluconobacter sp009547075	86.9651	725	1075	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018256155.1	s__Gluconobacter sp018256155	86.9288	798	1075	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_006539125.1	s__Gluconobacter sphaericus	86.4396	718	1075	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	98.91	98.17	0.94	0.92	6	-
GCF_015244565.1	s__Gluconobacter vitians	86.2313	760	1075	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_007988905.1	s__Gluconobacter kanchanaburiensis	85.6431	678	1075	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter	95.0	100.00	100.00	1.00	1.00	2	-
GCF_014132155.1	s__Gluconobacter_A sp014132155	80.1639	417	1075	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconobacter_A	95.0	97.46	96.68	0.90	0.89	4	-
--------------------------------------------------------------------------------
[2024-01-24 11:21:19,940] [INFO] GTDB search result was written to GCF_030160195.1_ASM3016019v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:21:19,940] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:21:19,943] [INFO] DFAST_QC result json was written to GCF_030160195.1_ASM3016019v1_genomic.fna/dqc_result.json
[2024-01-24 11:21:19,943] [INFO] DFAST_QC completed!
[2024-01-24 11:21:19,943] [INFO] Total running time: 0h1m0s