[2024-01-25 18:58:50,429] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:58:50,430] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:58:50,430] [INFO] DQC Reference Directory: /var/lib/cwl/stg3bf7496e-f306-4a29-a9c0-67fb832cce98/dqc_reference
[2024-01-25 18:58:51,566] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:58:51,567] [INFO] Task started: Prodigal
[2024-01-25 18:58:51,567] [INFO] Running command: gunzip -c /var/lib/cwl/stgca3c9b26-8a59-411a-b8b3-3350bfbeb700/GCF_030160595.1_ASM3016059v1_genomic.fna.gz | prodigal -d GCF_030160595.1_ASM3016059v1_genomic.fna/cds.fna -a GCF_030160595.1_ASM3016059v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:59:11,165] [INFO] Task succeeded: Prodigal
[2024-01-25 18:59:11,166] [INFO] Task started: HMMsearch
[2024-01-25 18:59:11,166] [INFO] Running command: hmmsearch --tblout GCF_030160595.1_ASM3016059v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3bf7496e-f306-4a29-a9c0-67fb832cce98/dqc_reference/reference_markers.hmm GCF_030160595.1_ASM3016059v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:59:11,470] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:59:11,471] [INFO] Found 6/6 markers.
[2024-01-25 18:59:11,528] [INFO] Query marker FASTA was written to GCF_030160595.1_ASM3016059v1_genomic.fna/markers.fasta
[2024-01-25 18:59:11,528] [INFO] Task started: Blastn
[2024-01-25 18:59:11,528] [INFO] Running command: blastn -query GCF_030160595.1_ASM3016059v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3bf7496e-f306-4a29-a9c0-67fb832cce98/dqc_reference/reference_markers.fasta -out GCF_030160595.1_ASM3016059v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:59:12,468] [INFO] Task succeeded: Blastn
[2024-01-25 18:59:12,471] [INFO] Selected 13 target genomes.
[2024-01-25 18:59:12,471] [INFO] Target genome list was writen to GCF_030160595.1_ASM3016059v1_genomic.fna/target_genomes.txt
[2024-01-25 18:59:12,478] [INFO] Task started: fastANI
[2024-01-25 18:59:12,478] [INFO] Running command: fastANI --query /var/lib/cwl/stgca3c9b26-8a59-411a-b8b3-3350bfbeb700/GCF_030160595.1_ASM3016059v1_genomic.fna.gz --refList GCF_030160595.1_ASM3016059v1_genomic.fna/target_genomes.txt --output GCF_030160595.1_ASM3016059v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:59:34,782] [INFO] Task succeeded: fastANI
[2024-01-25 18:59:34,783] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3bf7496e-f306-4a29-a9c0-67fb832cce98/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:59:34,783] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3bf7496e-f306-4a29-a9c0-67fb832cce98/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:59:34,796] [INFO] Found 13 fastANI hits (4 hits with ANI > threshold)
[2024-01-25 18:59:34,796] [INFO] The taxonomy check result is classified as 'inconclusive'.
[2024-01-25 18:59:34,796] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Rhizobium indigoferae	strain=CCBAU 71042	GCA_013087605.1	158891	158891	type	True	99.9944	2450	2489	95	inconclusive
Rhizobium leguminosarum	strain=USDA 2370	GCA_002008365.1	384	384	suspected-type	True	97.732	2159	2489	95	inconclusive
Rhizobium leguminosarum	strain=DSM 106839	GCA_014202125.1	384	384	suspected-type	True	97.7181	2155	2489	95	inconclusive
Rhizobium leguminosarum	strain=USDA 2370	GCA_003058385.1	384	384	suspected-type	True	97.7174	2162	2489	95	inconclusive
Rhizobium ruizarguesonis	strain=UPM1133	GCA_012349115.1	2081791	2081791	type	True	93.2475	2023	2489	95	below_threshold
Rhizobium indicum	strain=JKLM 12A2	GCA_005862305.2	2583231	2583231	type	True	92.8348	1976	2489	95	below_threshold
Rhizobium sophorae	strain=CCBAU 03386	GCA_013087515.1	1535242	1535242	type	True	92.408	1929	2489	95	below_threshold
Rhizobium changzhiense	strain=WYCCWR 11279	GCA_013087625.1	2692317	2692317	type	True	92.3974	1817	2489	95	below_threshold
Rhizobium redzepovicii	strain=18T	GCA_019793435.1	2867518	2867518	type	True	88.9861	1766	2489	95	below_threshold
Rhizobium croatiense	strain=13T	GCA_019793465.1	2867516	2867516	type	True	88.018	1643	2489	95	below_threshold
Rhizobium mongolense subsp. loessense	strain=CGMCC 1.3401	GCA_900099775.1	158890	57676	type	True	81.7351	1232	2489	95	below_threshold
Rhizobium dioscoreae	strain=S-93	GCA_009176305.1	2653122	2653122	type	True	80.1801	1023	2489	95	below_threshold
Shinella sumterensis	strain=MEC087	GCA_004514425.2	1967501	1967501	type	True	79.9042	734	2489	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:59:34,798] [INFO] DFAST Taxonomy check result was written to GCF_030160595.1_ASM3016059v1_genomic.fna/tc_result.tsv
[2024-01-25 18:59:34,799] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:59:34,799] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:59:34,799] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3bf7496e-f306-4a29-a9c0-67fb832cce98/dqc_reference/checkm_data
[2024-01-25 18:59:34,800] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:59:34,876] [INFO] Task started: CheckM
[2024-01-25 18:59:34,877] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_030160595.1_ASM3016059v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_030160595.1_ASM3016059v1_genomic.fna/checkm_input GCF_030160595.1_ASM3016059v1_genomic.fna/checkm_result
[2024-01-25 19:00:28,989] [INFO] Task succeeded: CheckM
[2024-01-25 19:00:28,991] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 19:00:29,011] [INFO] ===== Completeness check finished =====
[2024-01-25 19:00:29,011] [INFO] ===== Start GTDB Search =====
[2024-01-25 19:00:29,012] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_030160595.1_ASM3016059v1_genomic.fna/markers.fasta)
[2024-01-25 19:00:29,012] [INFO] Task started: Blastn
[2024-01-25 19:00:29,012] [INFO] Running command: blastn -query GCF_030160595.1_ASM3016059v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3bf7496e-f306-4a29-a9c0-67fb832cce98/dqc_reference/reference_markers_gtdb.fasta -out GCF_030160595.1_ASM3016059v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:00:31,012] [INFO] Task succeeded: Blastn
[2024-01-25 19:00:31,015] [INFO] Selected 10 target genomes.
[2024-01-25 19:00:31,015] [INFO] Target genome list was writen to GCF_030160595.1_ASM3016059v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 19:00:31,042] [INFO] Task started: fastANI
[2024-01-25 19:00:31,042] [INFO] Running command: fastANI --query /var/lib/cwl/stgca3c9b26-8a59-411a-b8b3-3350bfbeb700/GCF_030160595.1_ASM3016059v1_genomic.fna.gz --refList GCF_030160595.1_ASM3016059v1_genomic.fna/target_genomes_gtdb.txt --output GCF_030160595.1_ASM3016059v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 19:00:51,159] [INFO] Task succeeded: fastANI
[2024-01-25 19:00:51,166] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 19:00:51,167] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002008365.1	s__Rhizobium leguminosarum	97.7428	2158	2489	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Rhizobium	95.0	98.98	97.38	0.91	0.86	85	conclusive
GCF_002953715.1	s__Rhizobium leguminosarum_K	94.4933	2109	2489	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Rhizobium	95.0	95.64	95.51	0.85	0.83	10	-
GCF_001657485.1	s__Rhizobium sp001657485	93.4031	1955	2489	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Rhizobium	95.0	97.95	97.95	0.90	0.90	2	-
GCF_000769405.2	s__Rhizobium leguminosarum_E	93.2912	1948	2489	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Rhizobium	95.0	97.71	96.49	0.91	0.87	37	-
GCF_012349115.1	s__Rhizobium ruizarguesonis	93.2566	2022	2489	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Rhizobium	95.0	98.01	96.15	0.89	0.84	170	-
GCF_005862305.2	s__Rhizobium indicum	92.8277	1977	2489	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Rhizobium	95.0	97.44	95.15	0.91	0.82	6	-
GCF_004123835.1	s__Rhizobium leguminosarum_M	92.7743	1880	2489	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Rhizobium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000023185.1	s__Rhizobium leguminosarum_J	92.6617	1936	2489	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Rhizobium	95.0	98.61	97.68	0.92	0.88	6	-
GCF_000009265.1	s__Rhizobium leguminosarum_L	92.6558	1976	2489	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Rhizobium	95.0	98.59	95.46	0.90	0.82	60	-
GCF_013087515.1	s__Rhizobium sophorae	92.4253	1927	2489	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Rhizobium	95.0	96.85	95.49	0.87	0.83	6	-
--------------------------------------------------------------------------------
[2024-01-25 19:00:51,168] [INFO] GTDB search result was written to GCF_030160595.1_ASM3016059v1_genomic.fna/result_gtdb.tsv
[2024-01-25 19:00:51,168] [INFO] ===== GTDB Search completed =====
[2024-01-25 19:00:51,177] [INFO] DFAST_QC result json was written to GCF_030160595.1_ASM3016059v1_genomic.fna/dqc_result.json
[2024-01-25 19:00:51,177] [INFO] DFAST_QC completed!
[2024-01-25 19:00:51,177] [INFO] Total running time: 0h2m1s