SAMN32083172 Candidatus Chlorohelix allophototropha, single colony recovered from enrichment culture (subculture 19.9) SRS18088576 Candidatus Chlorohelix allophototropha "Candidatus Chlorohelix allophototropha" is a mesophilic and phototrophic member of the Chloroflexota (formerly Chloroflexi) phylum cultivated from an iron-rich Boreal Shield lake (Canada). The species is currently (as of 2022) unique among the Chloroflexota phylum in its use of a Type I photosynthetic reaction center (RCI) to perform light energy conversion. Here, we picked a single, isolated colony of "Ca. Chx. allophototropha" from a non-axenic enrichment culture (subculture 19.9) and extracted its DNA. Based on 16S rRNA gene amplicon sequencing, only sequences of "Ca. Chx. allophototropha" were detectable in the sample (to a detection limit of ~0.03% relative abundance). Given that the sample was dominated by "Ca. Chx. allophototropha", we used the DNA from this sample to obtain the genome of "Ca. Chx. allophototropha". Keywords: GSC:MIxS;MIGS:6.0 University of Waterloo Jackson Tsuji Makoto MIGS.ba MIGS/MIMS/MIMARKS.water MIGS.ba.water.6.0 2017-09 3.88 m small freshwater lake biome [ENVO_00000892] freshwater environment [ENVO_01000306] anoxic water [ENVO_01000173]|fresh water [ENVO_00002011] Canada: near Kenora, Ontario, Lake 227, IISD-Experimental Lakes Area https://doi.org/10.1101/2020.07.07.190934 49.69 N 93.69 W 5 https://doi.org/10.1101/2020.07.07.190934 Single colony of Candidatus Chlorohelix allophototropha from a non-axenic enrichment (subculture 19.9); enrichment culture was started from water from the upper anoxic zone (3.88 m depth) of an iron-rich Boreal Shield lake reference material of Candidatus Chlorohelix allophototropha L227-S17 909349