[2024-01-24 13:31:42,845] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:31:42,847] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:31:42,847] [INFO] DQC Reference Directory: /var/lib/cwl/stg2be78412-81f8-4c25-a189-a7e89d3403fb/dqc_reference
[2024-01-24 13:31:44,105] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:31:44,105] [INFO] Task started: Prodigal
[2024-01-24 13:31:44,106] [INFO] Running command: gunzip -c /var/lib/cwl/stgc8c6dd8b-117c-4b57-945c-9f2fee997f36/GCF_030506285.1_ASM3050628v1_genomic.fna.gz | prodigal -d GCF_030506285.1_ASM3050628v1_genomic.fna/cds.fna -a GCF_030506285.1_ASM3050628v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:32:15,662] [INFO] Task succeeded: Prodigal
[2024-01-24 13:32:15,662] [INFO] Task started: HMMsearch
[2024-01-24 13:32:15,662] [INFO] Running command: hmmsearch --tblout GCF_030506285.1_ASM3050628v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg2be78412-81f8-4c25-a189-a7e89d3403fb/dqc_reference/reference_markers.hmm GCF_030506285.1_ASM3050628v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:32:16,019] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:32:16,020] [INFO] Found 6/6 markers.
[2024-01-24 13:32:16,090] [INFO] Query marker FASTA was written to GCF_030506285.1_ASM3050628v1_genomic.fna/markers.fasta
[2024-01-24 13:32:16,091] [INFO] Task started: Blastn
[2024-01-24 13:32:16,091] [INFO] Running command: blastn -query GCF_030506285.1_ASM3050628v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2be78412-81f8-4c25-a189-a7e89d3403fb/dqc_reference/reference_markers.fasta -out GCF_030506285.1_ASM3050628v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:32:16,715] [INFO] Task succeeded: Blastn
[2024-01-24 13:32:16,719] [INFO] Selected 28 target genomes.
[2024-01-24 13:32:16,719] [INFO] Target genome list was writen to GCF_030506285.1_ASM3050628v1_genomic.fna/target_genomes.txt
[2024-01-24 13:32:16,732] [INFO] Task started: fastANI
[2024-01-24 13:32:16,733] [INFO] Running command: fastANI --query /var/lib/cwl/stgc8c6dd8b-117c-4b57-945c-9f2fee997f36/GCF_030506285.1_ASM3050628v1_genomic.fna.gz --refList GCF_030506285.1_ASM3050628v1_genomic.fna/target_genomes.txt --output GCF_030506285.1_ASM3050628v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:32:38,590] [INFO] Task succeeded: fastANI
[2024-01-24 13:32:38,591] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg2be78412-81f8-4c25-a189-a7e89d3403fb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:32:38,591] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg2be78412-81f8-4c25-a189-a7e89d3403fb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:32:38,593] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2024-01-24 13:32:38,593] [INFO] The taxonomy check result is classified as 'no_hit'.
[2024-01-24 13:32:38,593] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2024-01-24 13:32:38,595] [INFO] DFAST Taxonomy check result was written to GCF_030506285.1_ASM3050628v1_genomic.fna/tc_result.tsv
[2024-01-24 13:32:38,596] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:32:38,596] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:32:38,596] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg2be78412-81f8-4c25-a189-a7e89d3403fb/dqc_reference/checkm_data
[2024-01-24 13:32:38,600] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:32:38,672] [INFO] Task started: CheckM
[2024-01-24 13:32:38,672] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_030506285.1_ASM3050628v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_030506285.1_ASM3050628v1_genomic.fna/checkm_input GCF_030506285.1_ASM3050628v1_genomic.fna/checkm_result
[2024-01-24 13:34:06,151] [INFO] Task succeeded: CheckM
[2024-01-24 13:34:06,153] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:34:06,178] [INFO] ===== Completeness check finished =====
[2024-01-24 13:34:06,178] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:34:06,179] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_030506285.1_ASM3050628v1_genomic.fna/markers.fasta)
[2024-01-24 13:34:06,179] [INFO] Task started: Blastn
[2024-01-24 13:34:06,179] [INFO] Running command: blastn -query GCF_030506285.1_ASM3050628v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2be78412-81f8-4c25-a189-a7e89d3403fb/dqc_reference/reference_markers_gtdb.fasta -out GCF_030506285.1_ASM3050628v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:34:07,010] [INFO] Task succeeded: Blastn
[2024-01-24 13:34:07,015] [INFO] Selected 35 target genomes.
[2024-01-24 13:34:07,015] [INFO] Target genome list was writen to GCF_030506285.1_ASM3050628v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:34:07,076] [INFO] Task started: fastANI
[2024-01-24 13:34:07,076] [INFO] Running command: fastANI --query /var/lib/cwl/stgc8c6dd8b-117c-4b57-945c-9f2fee997f36/GCF_030506285.1_ASM3050628v1_genomic.fna.gz --refList GCF_030506285.1_ASM3050628v1_genomic.fna/target_genomes_gtdb.txt --output GCF_030506285.1_ASM3050628v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:34:36,966] [INFO] Task succeeded: fastANI
[2024-01-24 13:34:36,969] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2024-01-24 13:34:36,969] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2024-01-24 13:34:36,971] [INFO] GTDB search result was written to GCF_030506285.1_ASM3050628v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:34:36,971] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:34:36,974] [INFO] DFAST_QC result json was written to GCF_030506285.1_ASM3050628v1_genomic.fna/dqc_result.json
[2024-01-24 13:34:36,974] [INFO] DFAST_QC completed!
[2024-01-24 13:34:36,974] [INFO] Total running time: 0h2m54s