[2024-01-24 13:40:27,246] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:40:27,247] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:40:27,247] [INFO] DQC Reference Directory: /var/lib/cwl/stga2d1f83c-eca7-4cab-8f8f-5b33d6417dfc/dqc_reference
[2024-01-24 13:40:28,597] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:40:28,598] [INFO] Task started: Prodigal
[2024-01-24 13:40:28,598] [INFO] Running command: gunzip -c /var/lib/cwl/stg71a7bdb3-fbdd-4eac-b90a-fec57e23c02c/GCF_031001655.1_ASM3100165v1_genomic.fna.gz | prodigal -d GCF_031001655.1_ASM3100165v1_genomic.fna/cds.fna -a GCF_031001655.1_ASM3100165v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:40:52,096] [INFO] Task succeeded: Prodigal
[2024-01-24 13:40:52,096] [INFO] Task started: HMMsearch
[2024-01-24 13:40:52,096] [INFO] Running command: hmmsearch --tblout GCF_031001655.1_ASM3100165v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga2d1f83c-eca7-4cab-8f8f-5b33d6417dfc/dqc_reference/reference_markers.hmm GCF_031001655.1_ASM3100165v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:40:52,484] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:40:52,485] [INFO] Found 6/6 markers.
[2024-01-24 13:40:52,524] [INFO] Query marker FASTA was written to GCF_031001655.1_ASM3100165v1_genomic.fna/markers.fasta
[2024-01-24 13:40:52,524] [INFO] Task started: Blastn
[2024-01-24 13:40:52,525] [INFO] Running command: blastn -query GCF_031001655.1_ASM3100165v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga2d1f83c-eca7-4cab-8f8f-5b33d6417dfc/dqc_reference/reference_markers.fasta -out GCF_031001655.1_ASM3100165v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:40:53,192] [INFO] Task succeeded: Blastn
[2024-01-24 13:40:53,195] [INFO] Selected 12 target genomes.
[2024-01-24 13:40:53,196] [INFO] Target genome list was writen to GCF_031001655.1_ASM3100165v1_genomic.fna/target_genomes.txt
[2024-01-24 13:40:53,201] [INFO] Task started: fastANI
[2024-01-24 13:40:53,201] [INFO] Running command: fastANI --query /var/lib/cwl/stg71a7bdb3-fbdd-4eac-b90a-fec57e23c02c/GCF_031001655.1_ASM3100165v1_genomic.fna.gz --refList GCF_031001655.1_ASM3100165v1_genomic.fna/target_genomes.txt --output GCF_031001655.1_ASM3100165v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:41:05,650] [INFO] Task succeeded: fastANI
[2024-01-24 13:41:05,651] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga2d1f83c-eca7-4cab-8f8f-5b33d6417dfc/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:41:05,651] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga2d1f83c-eca7-4cab-8f8f-5b33d6417dfc/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:41:05,661] [INFO] Found 11 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 13:41:05,661] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:41:05,662] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Phocaeicola massiliensis	strain=DSM 17679	GCA_000382445.1	204516	204516	suspected-type	True	99.9797	1435	1439	95	conclusive
Phocaeicola massiliensis	strain=DSM 17679	GCA_000373085.1	204516	204516	type	True	99.9794	1421	1439	95	conclusive
Phocaeicola faecalis	strain=FXJYN30E22	GCA_021730445.1	2786956	2786956	type	True	90.3892	1021	1439	95	below_threshold
Phocaeicola vulgatus	strain=ATCC 8482	GCA_000012825.1	821	821	type	True	82.6231	616	1439	95	below_threshold
Phocaeicola vulgatus	strain=FDAARGOS_1098	GCA_016766915.1	821	821	type	True	82.5578	621	1439	95	below_threshold
Phocaeicola dorei	strain=DSM 17855	GCA_025146415.1	357276	357276	type	True	81.9377	613	1439	95	below_threshold
Phocaeicola dorei	strain=DSM 17855	GCA_013009555.1	357276	357276	type	True	81.8539	621	1439	95	below_threshold
Phocaeicola sartorii	strain=JCM 17136	GCA_000614185.1	671267	671267	type	True	80.7658	563	1439	95	below_threshold
Phocaeicola sartorii	strain=DSM 21941	GCA_024622025.1	671267	671267	type	True	80.7061	576	1439	95	below_threshold
Phocaeicola faecicola	strain=AGMB03916	GCA_013618865.1	2739389	2739389	type	True	78.9644	211	1439	95	below_threshold
Phocaeicola coprocola	strain=DSM 17136	GCA_000154845.1	310298	310298	suspected-type	True	78.9202	251	1439	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:41:05,663] [INFO] DFAST Taxonomy check result was written to GCF_031001655.1_ASM3100165v1_genomic.fna/tc_result.tsv
[2024-01-24 13:41:05,664] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:41:05,664] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:41:05,664] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga2d1f83c-eca7-4cab-8f8f-5b33d6417dfc/dqc_reference/checkm_data
[2024-01-24 13:41:05,665] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:41:05,716] [INFO] Task started: CheckM
[2024-01-24 13:41:05,716] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_031001655.1_ASM3100165v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_031001655.1_ASM3100165v1_genomic.fna/checkm_input GCF_031001655.1_ASM3100165v1_genomic.fna/checkm_result
[2024-01-24 13:42:10,232] [INFO] Task succeeded: CheckM
[2024-01-24 13:42:10,233] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:42:10,254] [INFO] ===== Completeness check finished =====
[2024-01-24 13:42:10,254] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:42:10,254] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_031001655.1_ASM3100165v1_genomic.fna/markers.fasta)
[2024-01-24 13:42:10,255] [INFO] Task started: Blastn
[2024-01-24 13:42:10,255] [INFO] Running command: blastn -query GCF_031001655.1_ASM3100165v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga2d1f83c-eca7-4cab-8f8f-5b33d6417dfc/dqc_reference/reference_markers_gtdb.fasta -out GCF_031001655.1_ASM3100165v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:42:11,084] [INFO] Task succeeded: Blastn
[2024-01-24 13:42:11,087] [INFO] Selected 9 target genomes.
[2024-01-24 13:42:11,087] [INFO] Target genome list was writen to GCF_031001655.1_ASM3100165v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:42:11,111] [INFO] Task started: fastANI
[2024-01-24 13:42:11,112] [INFO] Running command: fastANI --query /var/lib/cwl/stg71a7bdb3-fbdd-4eac-b90a-fec57e23c02c/GCF_031001655.1_ASM3100165v1_genomic.fna.gz --refList GCF_031001655.1_ASM3100165v1_genomic.fna/target_genomes_gtdb.txt --output GCF_031001655.1_ASM3100165v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:42:20,030] [INFO] Task succeeded: fastANI
[2024-01-24 13:42:20,038] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:42:20,039] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000382445.1	s__Phocaeicola massiliensis	99.9797	1435	1439	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Phocaeicola	95.0	98.84	98.03	0.86	0.62	25	conclusive
GCA_900760795.1	s__Phocaeicola sp900760795	94.0999	750	1439	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Phocaeicola	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002493165.1	s__Phocaeicola sp002493165	90.0816	897	1439	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Phocaeicola	95.0	99.20	98.75	0.86	0.83	5	-
GCF_000012825.1	s__Phocaeicola vulgatus	82.6226	616	1439	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Phocaeicola	95.359	98.84	95.43	0.81	0.61	263	-
GCF_013009555.1	s__Phocaeicola dorei	81.8382	622	1439	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Phocaeicola	95.359	99.04	97.27	0.84	0.54	104	-
GCA_011959205.1	s__Phocaeicola sp011959205	81.0133	493	1439	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Phocaeicola	95.0	99.87	99.87	0.90	0.90	2	-
GCF_000614185.1	s__Phocaeicola sartorii	80.7946	560	1439	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Phocaeicola	95.0	98.95	98.44	0.85	0.76	7	-
GCA_902388365.1	s__Phocaeicola sp902388365	80.384	556	1439	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Phocaeicola	95.0	98.75	97.52	0.90	0.83	3	-
GCA_000432735.1	s__Phocaeicola sp000432735	77.4015	177	1439	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Phocaeicola	95.0	98.41	98.37	0.86	0.85	3	-
--------------------------------------------------------------------------------
[2024-01-24 13:42:20,040] [INFO] GTDB search result was written to GCF_031001655.1_ASM3100165v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:42:20,041] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:42:20,044] [INFO] DFAST_QC result json was written to GCF_031001655.1_ASM3100165v1_genomic.fna/dqc_result.json
[2024-01-24 13:42:20,044] [INFO] DFAST_QC completed!
[2024-01-24 13:42:20,044] [INFO] Total running time: 0h1m53s