[2024-01-24 13:28:13,319] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:28:13,321] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:28:13,321] [INFO] DQC Reference Directory: /var/lib/cwl/stg63a36412-b76a-4cf7-8448-afe1afb041db/dqc_reference
[2024-01-24 13:28:14,598] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:28:14,598] [INFO] Task started: Prodigal
[2024-01-24 13:28:14,599] [INFO] Running command: gunzip -c /var/lib/cwl/stg3d5bc45e-3690-4e37-9220-1c696da3adfb/GCF_031453085.1_ASM3145308v1_genomic.fna.gz | prodigal -d GCF_031453085.1_ASM3145308v1_genomic.fna/cds.fna -a GCF_031453085.1_ASM3145308v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:28:25,258] [INFO] Task succeeded: Prodigal
[2024-01-24 13:28:25,259] [INFO] Task started: HMMsearch
[2024-01-24 13:28:25,259] [INFO] Running command: hmmsearch --tblout GCF_031453085.1_ASM3145308v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg63a36412-b76a-4cf7-8448-afe1afb041db/dqc_reference/reference_markers.hmm GCF_031453085.1_ASM3145308v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:28:25,566] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:28:25,568] [INFO] Found 6/6 markers.
[2024-01-24 13:28:25,611] [INFO] Query marker FASTA was written to GCF_031453085.1_ASM3145308v1_genomic.fna/markers.fasta
[2024-01-24 13:28:25,612] [INFO] Task started: Blastn
[2024-01-24 13:28:25,612] [INFO] Running command: blastn -query GCF_031453085.1_ASM3145308v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg63a36412-b76a-4cf7-8448-afe1afb041db/dqc_reference/reference_markers.fasta -out GCF_031453085.1_ASM3145308v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:28:26,384] [INFO] Task succeeded: Blastn
[2024-01-24 13:28:26,387] [INFO] Selected 14 target genomes.
[2024-01-24 13:28:26,387] [INFO] Target genome list was writen to GCF_031453085.1_ASM3145308v1_genomic.fna/target_genomes.txt
[2024-01-24 13:28:26,398] [INFO] Task started: fastANI
[2024-01-24 13:28:26,398] [INFO] Running command: fastANI --query /var/lib/cwl/stg3d5bc45e-3690-4e37-9220-1c696da3adfb/GCF_031453085.1_ASM3145308v1_genomic.fna.gz --refList GCF_031453085.1_ASM3145308v1_genomic.fna/target_genomes.txt --output GCF_031453085.1_ASM3145308v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:28:36,312] [INFO] Task succeeded: fastANI
[2024-01-24 13:28:36,312] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg63a36412-b76a-4cf7-8448-afe1afb041db/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:28:36,312] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg63a36412-b76a-4cf7-8448-afe1afb041db/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:28:36,328] [INFO] Found 12 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 13:28:36,328] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:28:36,328] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Asaia bogorensis	strain=NBRC 16594	GCA_001547995.1	91915	91915	suspected-type	True	96.8822	1012	1099	95	conclusive
Asaia bogorensis	strain=NBRC 16594	GCA_007989265.1	91915	91915	suspected-type	True	96.8268	1006	1099	95	conclusive
Asaia lannensis	strain=NBRC 102526	GCA_024054035.1	415421	415421	type	True	82.9483	702	1099	95	below_threshold
Asaia siamensis	strain=CCM 7132	GCA_014635085.1	110479	110479	type	True	82.0178	643	1099	95	below_threshold
Asaia platycodi	strain=JCM 25414	GCA_000614545.1	610243	610243	type	True	81.6109	631	1099	95	below_threshold
Asaia prunellae	strain=JCM 25354	GCA_000613885.1	610245	610245	type	True	80.9021	528	1099	95	below_threshold
Asaia astilbis	strain=JCM 15831	GCA_000613845.1	610244	610244	type	True	80.6562	502	1099	95	below_threshold
Komagataeibacter saccharivorans	strain=LMG 1582	GCA_003207825.1	265959	265959	type	True	77.553	158	1099	95	below_threshold
Roseomonas haemaphysalidis	strain=546	GCA_017355405.1	2768162	2768162	type	True	77.3768	75	1099	95	below_threshold
Roseococcus microcysteis	strain=NIBR12	GCA_014764365.1	2771361	2771361	type	True	77.3656	73	1099	95	below_threshold
Roseococcus thiosulfatophilus	strain=RB-3	GCA_017311575.1	35813	35813	type	True	76.4375	68	1099	95	below_threshold
Roseococcus pinisoli	strain=XZZS9	GCA_018413645.1	2835040	2835040	type	True	76.4221	58	1099	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:28:36,332] [INFO] DFAST Taxonomy check result was written to GCF_031453085.1_ASM3145308v1_genomic.fna/tc_result.tsv
[2024-01-24 13:28:36,333] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:28:36,333] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:28:36,334] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg63a36412-b76a-4cf7-8448-afe1afb041db/dqc_reference/checkm_data
[2024-01-24 13:28:36,335] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:28:36,375] [INFO] Task started: CheckM
[2024-01-24 13:28:36,376] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_031453085.1_ASM3145308v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_031453085.1_ASM3145308v1_genomic.fna/checkm_input GCF_031453085.1_ASM3145308v1_genomic.fna/checkm_result
[2024-01-24 13:29:13,190] [INFO] Task succeeded: CheckM
[2024-01-24 13:29:13,191] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:29:13,204] [INFO] ===== Completeness check finished =====
[2024-01-24 13:29:13,204] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:29:13,204] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_031453085.1_ASM3145308v1_genomic.fna/markers.fasta)
[2024-01-24 13:29:13,204] [INFO] Task started: Blastn
[2024-01-24 13:29:13,205] [INFO] Running command: blastn -query GCF_031453085.1_ASM3145308v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg63a36412-b76a-4cf7-8448-afe1afb041db/dqc_reference/reference_markers_gtdb.fasta -out GCF_031453085.1_ASM3145308v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:29:14,305] [INFO] Task succeeded: Blastn
[2024-01-24 13:29:14,309] [INFO] Selected 10 target genomes.
[2024-01-24 13:29:14,309] [INFO] Target genome list was writen to GCF_031453085.1_ASM3145308v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:29:14,323] [INFO] Task started: fastANI
[2024-01-24 13:29:14,323] [INFO] Running command: fastANI --query /var/lib/cwl/stg3d5bc45e-3690-4e37-9220-1c696da3adfb/GCF_031453085.1_ASM3145308v1_genomic.fna.gz --refList GCF_031453085.1_ASM3145308v1_genomic.fna/target_genomes_gtdb.txt --output GCF_031453085.1_ASM3145308v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:29:21,480] [INFO] Task succeeded: fastANI
[2024-01-24 13:29:21,489] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:29:21,489] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001547995.1	s__Asaia bogorensis	96.8822	1012	1099	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Asaia	95.0	96.55	95.04	0.93	0.88	6	conclusive
GCF_900465345.1	s__Asaia bogorensis_A	94.3246	919	1099	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Asaia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003994335.1	s__Asaia sp003994335	82.7055	689	1099	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Asaia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014635085.1	s__Asaia siamensis	82.0178	643	1099	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Asaia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000614545.1	s__Asaia platycodi	81.6109	631	1099	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Asaia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000613885.1	s__Asaia prunellae	80.9021	528	1099	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Asaia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_007988945.1	s__Swaminathania salitolerans	80.6777	475	1099	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Swaminathania	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000613845.1	s__Asaia astilbis	80.6707	502	1099	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Asaia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014174275.1	s__Gluconacetobacter takamatsuzukensis	77.3976	182	1099	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Acetobacterales;f__Acetobacteraceae;g__Gluconacetobacter	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:29:21,493] [INFO] GTDB search result was written to GCF_031453085.1_ASM3145308v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:29:21,493] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:29:21,496] [INFO] DFAST_QC result json was written to GCF_031453085.1_ASM3145308v1_genomic.fna/dqc_result.json
[2024-01-24 13:29:21,497] [INFO] DFAST_QC completed!
[2024-01-24 13:29:21,497] [INFO] Total running time: 0h1m8s