[2024-01-24 12:46:49,443] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:46:49,445] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:46:49,445] [INFO] DQC Reference Directory: /var/lib/cwl/stg9abc667a-2e32-40a9-9371-d55961dc7804/dqc_reference
[2024-01-24 12:46:50,621] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:46:50,622] [INFO] Task started: Prodigal
[2024-01-24 12:46:50,622] [INFO] Running command: gunzip -c /var/lib/cwl/stg5e9bfb8a-e177-457e-b8fb-58ca9f7a1f09/GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna.gz | prodigal -d GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/cds.fna -a GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:47:03,441] [INFO] Task succeeded: Prodigal
[2024-01-24 12:47:03,442] [INFO] Task started: HMMsearch
[2024-01-24 12:47:03,442] [INFO] Running command: hmmsearch --tblout GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg9abc667a-2e32-40a9-9371-d55961dc7804/dqc_reference/reference_markers.hmm GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:47:03,734] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:47:03,736] [INFO] Found 6/6 markers.
[2024-01-24 12:47:03,776] [INFO] Query marker FASTA was written to GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/markers.fasta
[2024-01-24 12:47:03,776] [INFO] Task started: Blastn
[2024-01-24 12:47:03,776] [INFO] Running command: blastn -query GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/markers.fasta -db /var/lib/cwl/stg9abc667a-2e32-40a9-9371-d55961dc7804/dqc_reference/reference_markers.fasta -out GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:47:04,545] [INFO] Task succeeded: Blastn
[2024-01-24 12:47:04,549] [INFO] Selected 13 target genomes.
[2024-01-24 12:47:04,550] [INFO] Target genome list was writen to GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/target_genomes.txt
[2024-01-24 12:47:04,555] [INFO] Task started: fastANI
[2024-01-24 12:47:04,555] [INFO] Running command: fastANI --query /var/lib/cwl/stg5e9bfb8a-e177-457e-b8fb-58ca9f7a1f09/GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna.gz --refList GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/target_genomes.txt --output GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:47:24,444] [INFO] Task succeeded: fastANI
[2024-01-24 12:47:24,445] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg9abc667a-2e32-40a9-9371-d55961dc7804/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:47:24,445] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg9abc667a-2e32-40a9-9371-d55961dc7804/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:47:24,456] [INFO] Found 12 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 12:47:24,456] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:47:24,456] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Photorhabdus luminescens	strain=ATCC 29999	GCA_900102985.1	29488	29488	suspected-type	True	100.0	1729	1732	95	conclusive
Photorhabdus luminescens subsp. luminescens	strain=DSM 3368	GCA_001083805.1	171439	29488	type	True	99.9799	1723	1732	95	conclusive
Photorhabdus luminescens subsp. mexicana	strain=MEX47-22	GCA_004348775.1	2100167	29488	type	True	96.8189	1522	1732	95	conclusive
Photorhabdus kleinii	strain=DSM 23513	GCA_025384885.1	768034	768034	type	True	92.2676	1358	1732	95	below_threshold
Photorhabdus noenieputensis	strain=DSM 25462	GCA_023108895.1	1208607	1208607	type	True	92.1703	1435	1732	95	below_threshold
Photorhabdus aegyptia	strain=BA1	GCA_000612035.1	2805098	2805098	type	True	92.086	1295	1732	95	below_threshold
Photorhabdus caribbeanensis	strain=HG29	GCA_018448885.1	1004165	1004165	type	True	92.0459	1407	1732	95	below_threshold
Photorhabdus hindustanensis	strain=H1	GCA_002968995.1	2918802	2918802	type	True	91.6775	1451	1732	95	below_threshold
Photorhabdus hainanensis	strain=DSM 22397	GCA_018448965.1	1004166	1004166	type	True	91.6709	1457	1732	95	below_threshold
Photorhabdus kayaii	strain=DSM 15194	GCA_025384895.1	230088	230088	type	True	91.5042	1307	1732	95	below_threshold
Photorhabdus namnaonensis	strain=PB45.5	GCA_001684335.1	1851568	1851568	type	True	91.4501	1442	1732	95	below_threshold
Photorhabdus temperata	strain=DSM 14550	GCA_025384845.1	574560	574560	type	True	86.053	1210	1732	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:47:24,458] [INFO] DFAST Taxonomy check result was written to GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/tc_result.tsv
[2024-01-24 12:47:24,458] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:47:24,458] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:47:24,459] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg9abc667a-2e32-40a9-9371-d55961dc7804/dqc_reference/checkm_data
[2024-01-24 12:47:24,460] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:47:24,512] [INFO] Task started: CheckM
[2024-01-24 12:47:24,513] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/checkm_input GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/checkm_result
[2024-01-24 12:48:08,272] [INFO] Task succeeded: CheckM
[2024-01-24 12:48:08,274] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:48:08,291] [INFO] ===== Completeness check finished =====
[2024-01-24 12:48:08,292] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:48:08,292] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/markers.fasta)
[2024-01-24 12:48:08,292] [INFO] Task started: Blastn
[2024-01-24 12:48:08,292] [INFO] Running command: blastn -query GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/markers.fasta -db /var/lib/cwl/stg9abc667a-2e32-40a9-9371-d55961dc7804/dqc_reference/reference_markers_gtdb.fasta -out GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:48:09,162] [INFO] Task succeeded: Blastn
[2024-01-24 12:48:09,165] [INFO] Selected 11 target genomes.
[2024-01-24 12:48:09,165] [INFO] Target genome list was writen to GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:48:09,173] [INFO] Task started: fastANI
[2024-01-24 12:48:09,173] [INFO] Running command: fastANI --query /var/lib/cwl/stg5e9bfb8a-e177-457e-b8fb-58ca9f7a1f09/GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna.gz --refList GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/target_genomes_gtdb.txt --output GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:48:26,224] [INFO] Task succeeded: fastANI
[2024-01-24 12:48:26,233] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:48:26,234] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001083805.1	s__Photorhabdus luminescens	99.9799	1723	1732	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Photorhabdus	95.0	97.45	96.24	0.90	0.85	5	conclusive
GCF_018448935.1	s__Photorhabdus noenieputensis	92.1638	1433	1732	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Photorhabdus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018448885.1	s__Photorhabdus caribbeanensis	92.0723	1404	1732	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Photorhabdus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018448925.1	s__Photorhabdus akhurstii	91.7716	1476	1732	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Photorhabdus	95.2863	97.65	96.64	0.91	0.87	12	-
GCF_003545805.1	s__Photorhabdus sp003545805	91.751	1362	1732	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Photorhabdus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003287735.1	s__Photorhabdus bodei	91.5616	1336	1732	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Photorhabdus	95.0	97.51	96.35	0.89	0.86	10	-
GCF_001684335.1	s__Photorhabdus namnaonensis	91.437	1444	1732	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Photorhabdus	95.2863	N/A	N/A	N/A	N/A	1	-
GCF_003343245.1	s__Photorhabdus laumondii	91.2343	1449	1732	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Photorhabdus	95.0	99.39	97.32	0.96	0.88	22	-
GCF_011189505.1	s__Photorhabdus tasmaniensis	85.8409	1189	1732	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Photorhabdus	95.0	99.99	99.99	1.00	1.00	2	-
GCF_018448905.1	s__Photorhabdus heterorhabditis	85.4228	1205	1732	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Photorhabdus	95.0	98.62	97.27	0.91	0.86	4	-
GCF_000711895.1	s__Photorhabdus australis	85.2253	1164	1732	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Photorhabdus	95.0	97.53	97.53	0.89	0.89	2	-
--------------------------------------------------------------------------------
[2024-01-24 12:48:26,237] [INFO] GTDB search result was written to GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/result_gtdb.tsv
[2024-01-24 12:48:26,237] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:48:26,241] [INFO] DFAST_QC result json was written to GCF_900102985.1_IMG-taxon_2597490348_annotated_assembly_genomic.fna/dqc_result.json
[2024-01-24 12:48:26,241] [INFO] DFAST_QC completed!
[2024-01-24 12:48:26,241] [INFO] Total running time: 0h1m37s