[2024-01-25 19:38:05,583] [INFO] DFAST_QC pipeline started.
[2024-01-25 19:38:05,584] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 19:38:05,584] [INFO] DQC Reference Directory: /var/lib/cwl/stgce9c9267-f53f-4ad1-a7cc-7403be20732d/dqc_reference
[2024-01-25 19:38:06,669] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 19:38:06,670] [INFO] Task started: Prodigal
[2024-01-25 19:38:06,670] [INFO] Running command: gunzip -c /var/lib/cwl/stg38df4d1b-4681-4f9e-9712-5d68c0943627/GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna.gz | prodigal -d GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/cds.fna -a GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 19:38:09,632] [INFO] Task succeeded: Prodigal
[2024-01-25 19:38:09,632] [INFO] Task started: HMMsearch
[2024-01-25 19:38:09,633] [INFO] Running command: hmmsearch --tblout GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgce9c9267-f53f-4ad1-a7cc-7403be20732d/dqc_reference/reference_markers.hmm GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/protein.faa > /dev/null
[2024-01-25 19:38:09,819] [INFO] Task succeeded: HMMsearch
[2024-01-25 19:38:09,820] [INFO] Found 6/6 markers.
[2024-01-25 19:38:09,837] [INFO] Query marker FASTA was written to GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/markers.fasta
[2024-01-25 19:38:09,837] [INFO] Task started: Blastn
[2024-01-25 19:38:09,837] [INFO] Running command: blastn -query GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/markers.fasta -db /var/lib/cwl/stgce9c9267-f53f-4ad1-a7cc-7403be20732d/dqc_reference/reference_markers.fasta -out GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:38:10,380] [INFO] Task succeeded: Blastn
[2024-01-25 19:38:10,383] [INFO] Selected 24 target genomes.
[2024-01-25 19:38:10,383] [INFO] Target genome list was writen to GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/target_genomes.txt
[2024-01-25 19:38:10,395] [INFO] Task started: fastANI
[2024-01-25 19:38:10,395] [INFO] Running command: fastANI --query /var/lib/cwl/stg38df4d1b-4681-4f9e-9712-5d68c0943627/GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna.gz --refList GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/target_genomes.txt --output GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 19:38:18,544] [INFO] Task succeeded: fastANI
[2024-01-25 19:38:18,545] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgce9c9267-f53f-4ad1-a7cc-7403be20732d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 19:38:18,545] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgce9c9267-f53f-4ad1-a7cc-7403be20732d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 19:38:18,549] [INFO] Found 2 fastANI hits (2 hits with ANI > threshold)
[2024-01-25 19:38:18,549] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 19:38:18,549] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Aerococcus urinaehominis	strain=DSM 15634	GCA_900103385.1	128944	128944	type	True	100.0	568	572	95	conclusive
Aerococcus urinaehominis	strain=CCUG42038B	GCA_001543245.1	128944	128944	type	True	99.9983	571	572	95	conclusive
--------------------------------------------------------------------------------
[2024-01-25 19:38:18,551] [INFO] DFAST Taxonomy check result was written to GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/tc_result.tsv
[2024-01-25 19:38:18,552] [INFO] ===== Taxonomy check completed =====
[2024-01-25 19:38:18,552] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 19:38:18,552] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgce9c9267-f53f-4ad1-a7cc-7403be20732d/dqc_reference/checkm_data
[2024-01-25 19:38:18,553] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 19:38:18,575] [INFO] Task started: CheckM
[2024-01-25 19:38:18,575] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/checkm_input GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/checkm_result
[2024-01-25 19:38:33,828] [INFO] Task succeeded: CheckM
[2024-01-25 19:38:33,829] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 19:38:33,853] [INFO] ===== Completeness check finished =====
[2024-01-25 19:38:33,853] [INFO] ===== Start GTDB Search =====
[2024-01-25 19:38:33,853] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/markers.fasta)
[2024-01-25 19:38:33,854] [INFO] Task started: Blastn
[2024-01-25 19:38:33,854] [INFO] Running command: blastn -query GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/markers.fasta -db /var/lib/cwl/stgce9c9267-f53f-4ad1-a7cc-7403be20732d/dqc_reference/reference_markers_gtdb.fasta -out GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:38:34,628] [INFO] Task succeeded: Blastn
[2024-01-25 19:38:34,631] [INFO] Selected 28 target genomes.
[2024-01-25 19:38:34,631] [INFO] Target genome list was writen to GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 19:38:34,664] [INFO] Task started: fastANI
[2024-01-25 19:38:34,664] [INFO] Running command: fastANI --query /var/lib/cwl/stg38df4d1b-4681-4f9e-9712-5d68c0943627/GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna.gz --refList GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/target_genomes_gtdb.txt --output GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 19:38:45,365] [INFO] Task succeeded: fastANI
[2024-01-25 19:38:45,368] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 19:38:45,368] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001543245.1	s__Aerococcus urinaehominis	99.9983	571	572	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Aerococcaceae;g__Aerococcus	95.0	100.00	100.00	1.00	1.00	2	conclusive
--------------------------------------------------------------------------------
[2024-01-25 19:38:45,369] [INFO] GTDB search result was written to GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/result_gtdb.tsv
[2024-01-25 19:38:45,370] [INFO] ===== GTDB Search completed =====
[2024-01-25 19:38:45,372] [INFO] DFAST_QC result json was written to GCF_900103385.1_IMG-taxon_2619619005_annotated_assembly_genomic.fna/dqc_result.json
[2024-01-25 19:38:45,372] [INFO] DFAST_QC completed!
[2024-01-25 19:38:45,372] [INFO] Total running time: 0h0m40s