[2024-01-24 13:49:27,639] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:49:27,641] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:49:27,641] [INFO] DQC Reference Directory: /var/lib/cwl/stg14cd0650-e0a2-44d7-99ce-59b3391ff3da/dqc_reference
[2024-01-24 13:49:28,904] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:49:28,905] [INFO] Task started: Prodigal
[2024-01-24 13:49:28,905] [INFO] Running command: gunzip -c /var/lib/cwl/stg952ed005-cc97-427a-aa82-54906d2f031c/GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna.gz | prodigal -d GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/cds.fna -a GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:49:33,769] [INFO] Task succeeded: Prodigal
[2024-01-24 13:49:33,769] [INFO] Task started: HMMsearch
[2024-01-24 13:49:33,769] [INFO] Running command: hmmsearch --tblout GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg14cd0650-e0a2-44d7-99ce-59b3391ff3da/dqc_reference/reference_markers.hmm GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:49:34,023] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:49:34,025] [INFO] Found 6/6 markers.
[2024-01-24 13:49:34,060] [INFO] Query marker FASTA was written to GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/markers.fasta
[2024-01-24 13:49:34,060] [INFO] Task started: Blastn
[2024-01-24 13:49:34,060] [INFO] Running command: blastn -query GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/markers.fasta -db /var/lib/cwl/stg14cd0650-e0a2-44d7-99ce-59b3391ff3da/dqc_reference/reference_markers.fasta -out GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:49:34,623] [INFO] Task succeeded: Blastn
[2024-01-24 13:49:34,685] [INFO] Selected 6 target genomes.
[2024-01-24 13:49:34,685] [INFO] Target genome list was writen to GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/target_genomes.txt
[2024-01-24 13:49:34,689] [INFO] Task started: fastANI
[2024-01-24 13:49:34,689] [INFO] Running command: fastANI --query /var/lib/cwl/stg952ed005-cc97-427a-aa82-54906d2f031c/GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna.gz --refList GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/target_genomes.txt --output GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:49:40,442] [INFO] Task succeeded: fastANI
[2024-01-24 13:49:40,442] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg14cd0650-e0a2-44d7-99ce-59b3391ff3da/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:49:40,443] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg14cd0650-e0a2-44d7-99ce-59b3391ff3da/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:49:40,451] [INFO] Found 6 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 13:49:40,451] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:49:40,451] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Thermoanaerobacter uzonensis	strain=DSM 18761	GCA_900129115.1	447593	447593	type	True	100.0	783	786	95	conclusive
Thermoanaerobacter siderophilus	strain=SR4	GCA_000262445.1	106578	106578	type	True	94.4997	654	786	95	below_threshold
Thermoanaerobacter wiegelii	strain=Rt8.B1	GCA_000147695.3	46354	46354	type	True	93.6704	661	786	95	below_threshold
Thermoanaerobacter thermocopriae	strain=JCM 7501	GCA_001310975.1	29350	29350	suspected-type	True	90.2684	557	786	95	below_threshold
Thermoanaerobacter thermocopriae	strain=ATCC 51646	GCA_000518565.1	29350	29350	suspected-type	True	90.1986	567	786	95	below_threshold
Thermoanaerobacter kivui	strain=DSM 2030; LKT-1	GCA_000763575.1	2325	2325	type	True	88.2442	544	786	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:49:40,453] [INFO] DFAST Taxonomy check result was written to GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/tc_result.tsv
[2024-01-24 13:49:40,454] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:49:40,454] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:49:40,454] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg14cd0650-e0a2-44d7-99ce-59b3391ff3da/dqc_reference/checkm_data
[2024-01-24 13:49:40,456] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:49:40,484] [INFO] Task started: CheckM
[2024-01-24 13:49:40,484] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/checkm_input GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/checkm_result
[2024-01-24 13:50:02,007] [INFO] Task succeeded: CheckM
[2024-01-24 13:50:02,008] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:50:02,028] [INFO] ===== Completeness check finished =====
[2024-01-24 13:50:02,029] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:50:02,029] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/markers.fasta)
[2024-01-24 13:50:02,030] [INFO] Task started: Blastn
[2024-01-24 13:50:02,030] [INFO] Running command: blastn -query GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/markers.fasta -db /var/lib/cwl/stg14cd0650-e0a2-44d7-99ce-59b3391ff3da/dqc_reference/reference_markers_gtdb.fasta -out GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:50:02,839] [INFO] Task succeeded: Blastn
[2024-01-24 13:50:02,842] [INFO] Selected 6 target genomes.
[2024-01-24 13:50:02,842] [INFO] Target genome list was writen to GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:50:02,847] [INFO] Task started: fastANI
[2024-01-24 13:50:02,847] [INFO] Running command: fastANI --query /var/lib/cwl/stg952ed005-cc97-427a-aa82-54906d2f031c/GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna.gz --refList GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/target_genomes_gtdb.txt --output GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:50:09,636] [INFO] Task succeeded: fastANI
[2024-01-24 13:50:09,643] [INFO] Found 6 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:50:09,643] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_900129115.1	s__Thermoanaerobacter uzonensis	100.0	783	786	d__Bacteria;p__Firmicutes_A;c__Thermoanaerobacteria;o__Thermoanaerobacterales;f__Thermoanaerobacteraceae;g__Thermoanaerobacter	95.0	96.59	96.59	0.87	0.87	2	conclusive
GCA_000445185.1	s__Thermoanaerobacter sp000445185	94.5855	601	786	d__Bacteria;p__Firmicutes_A;c__Thermoanaerobacteria;o__Thermoanaerobacterales;f__Thermoanaerobacteraceae;g__Thermoanaerobacter	95.9754	N/A	N/A	N/A	N/A	1	-
GCA_003722315.1	s__Thermoanaerobacter ethanolicus	94.3448	669	786	d__Bacteria;p__Firmicutes_A;c__Thermoanaerobacteria;o__Thermoanaerobacterales;f__Thermoanaerobacteraceae;g__Thermoanaerobacter	96.9409	98.23	97.61	0.86	0.74	8	-
GCF_000147695.2	s__Thermoanaerobacter wiegelii	93.6361	663	786	d__Bacteria;p__Firmicutes_A;c__Thermoanaerobacteria;o__Thermoanaerobacterales;f__Thermoanaerobacteraceae;g__Thermoanaerobacter	96.9409	N/A	N/A	N/A	N/A	1	-
GCF_000019085.1	s__Thermoanaerobacter pseudethanolicus	93.3319	576	786	d__Bacteria;p__Firmicutes_A;c__Thermoanaerobacteria;o__Thermoanaerobacterales;f__Thermoanaerobacteraceae;g__Thermoanaerobacter	95.1128	98.35	97.55	0.89	0.81	10	-
GCA_014896455.1	s__Thermoanaerobacter sp014896455	92.9398	575	786	d__Bacteria;p__Firmicutes_A;c__Thermoanaerobacteria;o__Thermoanaerobacterales;f__Thermoanaerobacteraceae;g__Thermoanaerobacter	95.6271	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:50:09,645] [INFO] GTDB search result was written to GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/result_gtdb.tsv
[2024-01-24 13:50:09,645] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:50:09,649] [INFO] DFAST_QC result json was written to GCF_900129115.1_IMG-taxon_2582581276_annotated_assembly_genomic.fna/dqc_result.json
[2024-01-24 13:50:09,649] [INFO] DFAST_QC completed!
[2024-01-24 13:50:09,649] [INFO] Total running time: 0h0m42s