[2024-01-24 13:13:46,492] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:13:46,494] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:13:46,494] [INFO] DQC Reference Directory: /var/lib/cwl/stge40567f9-5dbb-49b1-840b-67bda8ca0d2c/dqc_reference
[2024-01-24 13:13:47,705] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:13:47,705] [INFO] Task started: Prodigal
[2024-01-24 13:13:47,706] [INFO] Running command: gunzip -c /var/lib/cwl/stg669d60ff-2d12-4e6d-8cf3-28956a95337c/GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna.gz | prodigal -d GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/cds.fna -a GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:13:53,256] [INFO] Task succeeded: Prodigal
[2024-01-24 13:13:53,257] [INFO] Task started: HMMsearch
[2024-01-24 13:13:53,257] [INFO] Running command: hmmsearch --tblout GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stge40567f9-5dbb-49b1-840b-67bda8ca0d2c/dqc_reference/reference_markers.hmm GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:13:53,512] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:13:53,513] [INFO] Found 6/6 markers.
[2024-01-24 13:13:53,539] [INFO] Query marker FASTA was written to GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/markers.fasta
[2024-01-24 13:13:53,539] [INFO] Task started: Blastn
[2024-01-24 13:13:53,539] [INFO] Running command: blastn -query GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/markers.fasta -db /var/lib/cwl/stge40567f9-5dbb-49b1-840b-67bda8ca0d2c/dqc_reference/reference_markers.fasta -out GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:13:54,119] [INFO] Task succeeded: Blastn
[2024-01-24 13:13:54,123] [INFO] Selected 20 target genomes.
[2024-01-24 13:13:54,124] [INFO] Target genome list was writen to GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/target_genomes.txt
[2024-01-24 13:13:54,142] [INFO] Task started: fastANI
[2024-01-24 13:13:54,142] [INFO] Running command: fastANI --query /var/lib/cwl/stg669d60ff-2d12-4e6d-8cf3-28956a95337c/GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna.gz --refList GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/target_genomes.txt --output GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:14:05,150] [INFO] Task succeeded: fastANI
[2024-01-24 13:14:05,151] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stge40567f9-5dbb-49b1-840b-67bda8ca0d2c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:14:05,151] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stge40567f9-5dbb-49b1-840b-67bda8ca0d2c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:14:05,157] [INFO] Found 1 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 13:14:05,157] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:14:05,157] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Desulfovibrio litoralis	strain=DSM 11393	GCA_900143255.1	466107	466107	type	True	100.0	898	900	95	conclusive
--------------------------------------------------------------------------------
[2024-01-24 13:14:05,159] [INFO] DFAST Taxonomy check result was written to GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/tc_result.tsv
[2024-01-24 13:14:05,160] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:14:05,160] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:14:05,160] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stge40567f9-5dbb-49b1-840b-67bda8ca0d2c/dqc_reference/checkm_data
[2024-01-24 13:14:05,162] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:14:05,190] [INFO] Task started: CheckM
[2024-01-24 13:14:05,190] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/checkm_input GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/checkm_result
[2024-01-24 13:14:27,943] [INFO] Task succeeded: CheckM
[2024-01-24 13:14:27,944] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 98.15%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:14:27,971] [INFO] ===== Completeness check finished =====
[2024-01-24 13:14:27,972] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:14:27,972] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/markers.fasta)
[2024-01-24 13:14:27,973] [INFO] Task started: Blastn
[2024-01-24 13:14:27,973] [INFO] Running command: blastn -query GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/markers.fasta -db /var/lib/cwl/stge40567f9-5dbb-49b1-840b-67bda8ca0d2c/dqc_reference/reference_markers_gtdb.fasta -out GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:14:28,686] [INFO] Task succeeded: Blastn
[2024-01-24 13:14:28,690] [INFO] Selected 27 target genomes.
[2024-01-24 13:14:28,690] [INFO] Target genome list was writen to GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:14:28,713] [INFO] Task started: fastANI
[2024-01-24 13:14:28,713] [INFO] Running command: fastANI --query /var/lib/cwl/stg669d60ff-2d12-4e6d-8cf3-28956a95337c/GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna.gz --refList GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/target_genomes_gtdb.txt --output GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:14:39,512] [INFO] Task succeeded: fastANI
[2024-01-24 13:14:39,516] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:14:39,516] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_900143255.1	s__Frigididesulfovibrio_A litoralis	100.0	898	900	d__Bacteria;p__Desulfobacterota_I;c__Desulfovibrionia;o__Desulfovibrionales;f__Desulfovibrionaceae;g__Frigididesulfovibrio_A	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2024-01-24 13:14:39,519] [INFO] GTDB search result was written to GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/result_gtdb.tsv
[2024-01-24 13:14:39,520] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:14:39,522] [INFO] DFAST_QC result json was written to GCF_900143255.1_IMG-taxon_2582580730_annotated_assembly_genomic.fna/dqc_result.json
[2024-01-24 13:14:39,523] [INFO] DFAST_QC completed!
[2024-01-24 13:14:39,523] [INFO] Total running time: 0h0m53s