[2024-01-24 14:12:33,652] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:12:33,654] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:12:33,654] [INFO] DQC Reference Directory: /var/lib/cwl/stg92ab2ce3-466e-40ff-8776-dbb8d48658cd/dqc_reference
[2024-01-24 14:12:34,884] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:12:34,885] [INFO] Task started: Prodigal
[2024-01-24 14:12:34,885] [INFO] Running command: gunzip -c /var/lib/cwl/stgb9d554c7-d150-40d1-ba33-a8ec59af5566/GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna.gz | prodigal -d GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/cds.fna -a GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:12:38,853] [INFO] Task succeeded: Prodigal
[2024-01-24 14:12:38,853] [INFO] Task started: HMMsearch
[2024-01-24 14:12:38,853] [INFO] Running command: hmmsearch --tblout GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg92ab2ce3-466e-40ff-8776-dbb8d48658cd/dqc_reference/reference_markers.hmm GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:12:39,119] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:12:39,121] [INFO] Found 6/6 markers.
[2024-01-24 14:12:39,143] [INFO] Query marker FASTA was written to GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/markers.fasta
[2024-01-24 14:12:39,143] [INFO] Task started: Blastn
[2024-01-24 14:12:39,143] [INFO] Running command: blastn -query GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/markers.fasta -db /var/lib/cwl/stg92ab2ce3-466e-40ff-8776-dbb8d48658cd/dqc_reference/reference_markers.fasta -out GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:12:39,817] [INFO] Task succeeded: Blastn
[2024-01-24 14:12:39,821] [INFO] Selected 20 target genomes.
[2024-01-24 14:12:39,822] [INFO] Target genome list was writen to GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/target_genomes.txt
[2024-01-24 14:12:40,005] [INFO] Task started: fastANI
[2024-01-24 14:12:40,006] [INFO] Running command: fastANI --query /var/lib/cwl/stgb9d554c7-d150-40d1-ba33-a8ec59af5566/GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna.gz --refList GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/target_genomes.txt --output GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:12:48,204] [INFO] Task succeeded: fastANI
[2024-01-24 14:12:48,205] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg92ab2ce3-466e-40ff-8776-dbb8d48658cd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:12:48,205] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg92ab2ce3-466e-40ff-8776-dbb8d48658cd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:12:48,216] [INFO] Found 6 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 14:12:48,216] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:12:48,216] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Globicatella sulfidifaciens	strain=DSM 15739	GCA_900167405.1	136093	136093	type	True	100.0	715	718	95	conclusive
Globicatella sanguinis	strain=NBRC 15551	GCA_001552295.1	13076	13076	type	True	94.705	513	718	95	below_threshold
Vaginisenegalia massiliensis	strain=Marseille-P5643	GCA_900604935.1	2058294	2058294	type	True	80.0441	55	718	95	below_threshold
Fundicoccus ignavus	strain=DSM 109652	GCA_009659375.1	2664442	2664442	type	True	78.8541	91	718	95	below_threshold
Ruoffia halotolerans	strain=INB8	GCA_014049385.1	2748684	2748684	type	True	78.7196	67	718	95	below_threshold
Eremococcus coleocola	strain=DSM 15696	GCA_000428865.1	88132	88132	type	True	77.4491	61	718	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:12:48,218] [INFO] DFAST Taxonomy check result was written to GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/tc_result.tsv
[2024-01-24 14:12:48,218] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:12:48,219] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:12:48,219] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg92ab2ce3-466e-40ff-8776-dbb8d48658cd/dqc_reference/checkm_data
[2024-01-24 14:12:48,220] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:12:48,246] [INFO] Task started: CheckM
[2024-01-24 14:12:48,246] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/checkm_input GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/checkm_result
[2024-01-24 14:13:07,823] [INFO] Task succeeded: CheckM
[2024-01-24 14:13:07,825] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:13:07,846] [INFO] ===== Completeness check finished =====
[2024-01-24 14:13:07,847] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:13:07,847] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/markers.fasta)
[2024-01-24 14:13:07,848] [INFO] Task started: Blastn
[2024-01-24 14:13:07,848] [INFO] Running command: blastn -query GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/markers.fasta -db /var/lib/cwl/stg92ab2ce3-466e-40ff-8776-dbb8d48658cd/dqc_reference/reference_markers_gtdb.fasta -out GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:13:08,590] [INFO] Task succeeded: Blastn
[2024-01-24 14:13:08,594] [INFO] Selected 16 target genomes.
[2024-01-24 14:13:08,594] [INFO] Target genome list was writen to GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:13:08,611] [INFO] Task started: fastANI
[2024-01-24 14:13:08,611] [INFO] Running command: fastANI --query /var/lib/cwl/stgb9d554c7-d150-40d1-ba33-a8ec59af5566/GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna.gz --refList GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/target_genomes_gtdb.txt --output GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:13:15,500] [INFO] Task succeeded: fastANI
[2024-01-24 14:13:15,513] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:13:15,513] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_900167405.1	s__Globicatella sulfidifaciens	100.0	715	718	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Aerococcaceae;g__Globicatella	95.0	97.81	97.81	0.71	0.71	2	conclusive
GCF_001552295.1	s__Globicatella sanguinis	94.705	513	718	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Aerococcaceae;g__Globicatella	95.0	97.13	96.22	0.85	0.84	3	-
GCF_900604935.1	s__Abiotrophia massiliensis	79.9224	56	718	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Aerococcaceae;g__Abiotrophia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009659375.1	s__Fundicoccus ignavus	78.8541	91	718	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Aerococcaceae;g__Fundicoccus	95.0	98.16	98.10	0.87	0.87	3	-
GCA_015863215.1	s__DSM-111022 sp015863215	78.3232	57	718	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Aerococcaceae;g__DSM-111022	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016237705.1	s__Globicatella sp010892315	78.319	169	718	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Aerococcaceae;g__Globicatella	95.0	98.61	97.07	0.93	0.84	6	-
GCA_016126655.1	s__Globicatella sp016126655	78.2787	148	718	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Aerococcaceae;g__Globicatella	95.0	98.94	98.51	0.93	0.88	6	-
GCF_005864045.1	s__Facklamia_A tabacinasalis	78.0894	67	718	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Aerococcaceae;g__Facklamia_A	95.0	96.44	96.27	0.84	0.80	4	-
--------------------------------------------------------------------------------
[2024-01-24 14:13:15,518] [INFO] GTDB search result was written to GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/result_gtdb.tsv
[2024-01-24 14:13:15,518] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:13:15,521] [INFO] DFAST_QC result json was written to GCF_900167405.1_IMG-taxon_2524614644_annotated_assembly_genomic.fna/dqc_result.json
[2024-01-24 14:13:15,522] [INFO] DFAST_QC completed!
[2024-01-24 14:13:15,522] [INFO] Total running time: 0h0m42s