[2024-01-24 13:13:11,627] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:13:11,629] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:13:11,630] [INFO] DQC Reference Directory: /var/lib/cwl/stgca7177ee-d0ce-4b47-8132-a59d1369f178/dqc_reference
[2024-01-24 13:13:13,068] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:13:13,069] [INFO] Task started: Prodigal
[2024-01-24 13:13:13,070] [INFO] Running command: gunzip -c /var/lib/cwl/stg0d1c09ee-84bc-486b-9295-3eb6543050fe/GCF_900445245.1_52756_C01_genomic.fna.gz | prodigal -d GCF_900445245.1_52756_C01_genomic.fna/cds.fna -a GCF_900445245.1_52756_C01_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:13:23,170] [INFO] Task succeeded: Prodigal
[2024-01-24 13:13:23,171] [INFO] Task started: HMMsearch
[2024-01-24 13:13:23,171] [INFO] Running command: hmmsearch --tblout GCF_900445245.1_52756_C01_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgca7177ee-d0ce-4b47-8132-a59d1369f178/dqc_reference/reference_markers.hmm GCF_900445245.1_52756_C01_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:13:23,424] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:13:23,425] [INFO] Found 6/6 markers.
[2024-01-24 13:13:23,460] [INFO] Query marker FASTA was written to GCF_900445245.1_52756_C01_genomic.fna/markers.fasta
[2024-01-24 13:13:23,461] [INFO] Task started: Blastn
[2024-01-24 13:13:23,461] [INFO] Running command: blastn -query GCF_900445245.1_52756_C01_genomic.fna/markers.fasta -db /var/lib/cwl/stgca7177ee-d0ce-4b47-8132-a59d1369f178/dqc_reference/reference_markers.fasta -out GCF_900445245.1_52756_C01_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:13:24,157] [INFO] Task succeeded: Blastn
[2024-01-24 13:13:24,161] [INFO] Selected 12 target genomes.
[2024-01-24 13:13:24,161] [INFO] Target genome list was writen to GCF_900445245.1_52756_C01_genomic.fna/target_genomes.txt
[2024-01-24 13:13:24,165] [INFO] Task started: fastANI
[2024-01-24 13:13:24,165] [INFO] Running command: fastANI --query /var/lib/cwl/stg0d1c09ee-84bc-486b-9295-3eb6543050fe/GCF_900445245.1_52756_C01_genomic.fna.gz --refList GCF_900445245.1_52756_C01_genomic.fna/target_genomes.txt --output GCF_900445245.1_52756_C01_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:13:42,689] [INFO] Task succeeded: fastANI
[2024-01-24 13:13:42,690] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgca7177ee-d0ce-4b47-8132-a59d1369f178/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:13:42,690] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgca7177ee-d0ce-4b47-8132-a59d1369f178/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:13:42,700] [INFO] Found 12 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 13:13:42,700] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:13:42,701] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Alysiella crassa	strain=NCTC10283	GCA_900445245.1	153491	153491	type	True	100.0	935	940	95	conclusive
Alysiella crassa	strain=S6	GCA_022871045.1	153491	153491	type	True	99.9685	933	940	95	conclusive
Alysiella crassa	strain=DSM 2578	GCA_000745955.1	153491	153491	type	True	99.8816	827	940	95	conclusive
Alysiella filiformis	strain=DSM 16848	GCA_014054525.1	194196	194196	type	True	85.6752	508	940	95	below_threshold
Alysiella filiformis	strain=DSM 16848	GCA_020162295.1	194196	194196	type	True	85.6309	493	940	95	below_threshold
Alysiella filiformis	strain=DSM 16848	GCA_900230205.1	194196	194196	type	True	85.3334	497	940	95	below_threshold
Simonsiella muelleri	strain=ATCC 29453	GCA_002951835.1	72	72	type	True	79.9005	450	940	95	below_threshold
Kingella kingae	strain=4177/66	GCA_022871005.1	504	504	type	True	79.8995	356	940	95	below_threshold
Simonsiella muelleri	strain=ATCC 29453	GCA_000163775.2	72	72	type	True	79.898	447	940	95	below_threshold
Uruburuella suis	strain=1258/02	GCA_022870845.1	252130	252130	type	True	77.847	151	940	95	below_threshold
Uruburuella testudinis	strain=CCUG 63373m	GCA_022870865.1	1282863	1282863	type	True	77.5454	138	940	95	below_threshold
Uruburuella suis	strain=DSM 17474	GCA_004341385.1	252130	252130	type	True	76.9392	145	940	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:13:42,702] [INFO] DFAST Taxonomy check result was written to GCF_900445245.1_52756_C01_genomic.fna/tc_result.tsv
[2024-01-24 13:13:42,702] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:13:42,703] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:13:42,703] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgca7177ee-d0ce-4b47-8132-a59d1369f178/dqc_reference/checkm_data
[2024-01-24 13:13:42,704] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:13:42,733] [INFO] Task started: CheckM
[2024-01-24 13:13:42,734] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_900445245.1_52756_C01_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_900445245.1_52756_C01_genomic.fna/checkm_input GCF_900445245.1_52756_C01_genomic.fna/checkm_result
[2024-01-24 13:14:18,546] [INFO] Task succeeded: CheckM
[2024-01-24 13:14:18,548] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:14:18,562] [INFO] ===== Completeness check finished =====
[2024-01-24 13:14:18,562] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:14:18,563] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_900445245.1_52756_C01_genomic.fna/markers.fasta)
[2024-01-24 13:14:18,563] [INFO] Task started: Blastn
[2024-01-24 13:14:18,563] [INFO] Running command: blastn -query GCF_900445245.1_52756_C01_genomic.fna/markers.fasta -db /var/lib/cwl/stgca7177ee-d0ce-4b47-8132-a59d1369f178/dqc_reference/reference_markers_gtdb.fasta -out GCF_900445245.1_52756_C01_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:14:19,662] [INFO] Task succeeded: Blastn
[2024-01-24 13:14:19,665] [INFO] Selected 9 target genomes.
[2024-01-24 13:14:19,665] [INFO] Target genome list was writen to GCF_900445245.1_52756_C01_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:14:19,677] [INFO] Task started: fastANI
[2024-01-24 13:14:19,677] [INFO] Running command: fastANI --query /var/lib/cwl/stg0d1c09ee-84bc-486b-9295-3eb6543050fe/GCF_900445245.1_52756_C01_genomic.fna.gz --refList GCF_900445245.1_52756_C01_genomic.fna/target_genomes_gtdb.txt --output GCF_900445245.1_52756_C01_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:14:28,073] [INFO] Task succeeded: fastANI
[2024-01-24 13:14:28,088] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:14:28,089] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000745955.1	s__Alysiella crassa	99.8816	827	940	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Alysiella	95.0	99.94	99.94	0.99	0.99	2	conclusive
GCF_014054525.1	s__Alysiella filiformis	85.687	509	940	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Alysiella	95.0	99.98	99.98	1.00	1.00	2	-
GCF_002951835.1	s__Simonsiella muelleri	79.9688	447	940	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Simonsiella	95.0	99.93	99.93	0.98	0.98	2	-
GCF_900111845.1	s__Kingella kingae	79.6588	336	940	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Kingella	95.0	98.43	98.16	0.96	0.94	37	-
GCF_000751855.1	s__Kingella negevensis	79.4437	322	940	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Kingella	95.0	99.09	98.48	0.96	0.94	3	-
GCF_014054725.1	s__Conchiformibius steedae	78.7249	265	940	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Conchiformibius	95.0	98.09	96.18	0.94	0.87	3	-
GCF_014054985.1	s__Kingella_B oralis	78.3063	293	940	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Kingella_B	95.0	98.22	96.45	0.95	0.91	3	-
GCF_000428785.1	s__Conchiformibius kuhniae	77.9623	268	940	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Conchiformibius	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905373195.1	s__Kingella_B oralis_A	77.8426	177	940	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Kingella_B	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:14:28,091] [INFO] GTDB search result was written to GCF_900445245.1_52756_C01_genomic.fna/result_gtdb.tsv
[2024-01-24 13:14:28,092] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:14:28,095] [INFO] DFAST_QC result json was written to GCF_900445245.1_52756_C01_genomic.fna/dqc_result.json
[2024-01-24 13:14:28,095] [INFO] DFAST_QC completed!
[2024-01-24 13:14:28,095] [INFO] Total running time: 0h1m16s