[2024-01-24 11:51:06,406] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:51:06,408] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:51:06,408] [INFO] DQC Reference Directory: /var/lib/cwl/stg24ca7ae8-4cea-4f8d-a2ad-a7ad298633e7/dqc_reference
[2024-01-24 11:51:08,674] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:51:08,675] [INFO] Task started: Prodigal
[2024-01-24 11:51:08,676] [INFO] Running command: gunzip -c /var/lib/cwl/stg61880420-69f8-49cc-8d78-1f44d4115948/GCF_900458505.1_51395_E01_genomic.fna.gz | prodigal -d GCF_900458505.1_51395_E01_genomic.fna/cds.fna -a GCF_900458505.1_51395_E01_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:51:13,419] [INFO] Task succeeded: Prodigal
[2024-01-24 11:51:13,419] [INFO] Task started: HMMsearch
[2024-01-24 11:51:13,419] [INFO] Running command: hmmsearch --tblout GCF_900458505.1_51395_E01_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg24ca7ae8-4cea-4f8d-a2ad-a7ad298633e7/dqc_reference/reference_markers.hmm GCF_900458505.1_51395_E01_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:51:13,721] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:51:13,722] [INFO] Found 6/6 markers.
[2024-01-24 11:51:13,747] [INFO] Query marker FASTA was written to GCF_900458505.1_51395_E01_genomic.fna/markers.fasta
[2024-01-24 11:51:13,748] [INFO] Task started: Blastn
[2024-01-24 11:51:13,748] [INFO] Running command: blastn -query GCF_900458505.1_51395_E01_genomic.fna/markers.fasta -db /var/lib/cwl/stg24ca7ae8-4cea-4f8d-a2ad-a7ad298633e7/dqc_reference/reference_markers.fasta -out GCF_900458505.1_51395_E01_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:51:14,933] [INFO] Task succeeded: Blastn
[2024-01-24 11:51:14,936] [INFO] Selected 11 target genomes.
[2024-01-24 11:51:14,936] [INFO] Target genome list was writen to GCF_900458505.1_51395_E01_genomic.fna/target_genomes.txt
[2024-01-24 11:51:15,009] [INFO] Task started: fastANI
[2024-01-24 11:51:15,009] [INFO] Running command: fastANI --query /var/lib/cwl/stg61880420-69f8-49cc-8d78-1f44d4115948/GCF_900458505.1_51395_E01_genomic.fna.gz --refList GCF_900458505.1_51395_E01_genomic.fna/target_genomes.txt --output GCF_900458505.1_51395_E01_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:51:23,228] [INFO] Task succeeded: fastANI
[2024-01-24 11:51:23,228] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg24ca7ae8-4cea-4f8d-a2ad-a7ad298633e7/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:51:23,229] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg24ca7ae8-4cea-4f8d-a2ad-a7ad298633e7/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:51:23,236] [INFO] Found 6 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 11:51:23,237] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:51:23,237] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Mammaliicoccus fleurettii	strain=NCTC13829	GCA_900458505.1	150056	150056	type	True	100.0	891	894	95	conclusive
Mammaliicoccus fleurettii	strain=DSM 13212	GCA_003970575.1	150056	150056	type	True	99.7839	759	894	95	conclusive
Mammaliicoccus vitulinus	strain=FDAARGOS_1207	GCA_016889245.1	71237	71237	suspected-type	True	87.638	737	894	95	below_threshold
Mammaliicoccus vitulinus	strain=DSM 15615	GCA_002902265.1	71237	71237	type	True	87.5469	657	894	95	below_threshold
Mammaliicoccus sciuri	strain=NCTC12103	GCA_900474615.1	1296	1296	type	True	84.3645	675	894	95	below_threshold
Mammaliicoccus sciuri	strain=DSM 20345	GCA_001046995.1	1296	1296	type	True	84.1236	670	894	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:51:23,238] [INFO] DFAST Taxonomy check result was written to GCF_900458505.1_51395_E01_genomic.fna/tc_result.tsv
[2024-01-24 11:51:23,239] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:51:23,239] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:51:23,239] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg24ca7ae8-4cea-4f8d-a2ad-a7ad298633e7/dqc_reference/checkm_data
[2024-01-24 11:51:23,241] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:51:23,272] [INFO] Task started: CheckM
[2024-01-24 11:51:23,272] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_900458505.1_51395_E01_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_900458505.1_51395_E01_genomic.fna/checkm_input GCF_900458505.1_51395_E01_genomic.fna/checkm_result
[2024-01-24 11:51:44,294] [INFO] Task succeeded: CheckM
[2024-01-24 11:51:44,296] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:51:44,318] [INFO] ===== Completeness check finished =====
[2024-01-24 11:51:44,318] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:51:44,318] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_900458505.1_51395_E01_genomic.fna/markers.fasta)
[2024-01-24 11:51:44,319] [INFO] Task started: Blastn
[2024-01-24 11:51:44,319] [INFO] Running command: blastn -query GCF_900458505.1_51395_E01_genomic.fna/markers.fasta -db /var/lib/cwl/stg24ca7ae8-4cea-4f8d-a2ad-a7ad298633e7/dqc_reference/reference_markers_gtdb.fasta -out GCF_900458505.1_51395_E01_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:51:45,116] [INFO] Task succeeded: Blastn
[2024-01-24 11:51:45,119] [INFO] Selected 7 target genomes.
[2024-01-24 11:51:45,119] [INFO] Target genome list was writen to GCF_900458505.1_51395_E01_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:51:45,127] [INFO] Task started: fastANI
[2024-01-24 11:51:45,127] [INFO] Running command: fastANI --query /var/lib/cwl/stg61880420-69f8-49cc-8d78-1f44d4115948/GCF_900458505.1_51395_E01_genomic.fna.gz --refList GCF_900458505.1_51395_E01_genomic.fna/target_genomes_gtdb.txt --output GCF_900458505.1_51395_E01_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:51:50,075] [INFO] Task succeeded: fastANI
[2024-01-24 11:51:50,082] [INFO] Found 6 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:51:50,083] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003970575.1	s__Mammaliicoccus fleurettii	99.7839	759	894	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Mammaliicoccus	95.0	99.35	98.97	0.91	0.88	9	conclusive
GCF_002902265.1	s__Mammaliicoccus vitulinus	87.5402	657	894	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Mammaliicoccus	95.0	98.82	98.52	0.92	0.85	19	-
GCF_002901825.1	s__Mammaliicoccus sciuri	84.0023	671	894	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Mammaliicoccus	95.0	96.65	95.60	0.90	0.84	216	-
GCF_002902755.1	s__Mammaliicoccus lentus	81.6669	580	894	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Mammaliicoccus	95.0	99.03	97.66	0.94	0.90	21	-
GCF_002902525.1	s__Mammaliicoccus stepanovicii	81.4333	515	894	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Mammaliicoccus	95.0	100.00	100.00	1.00	0.99	3	-
GCF_002901845.1	s__Staphylococcus hominis	77.8298	178	894	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	97.87	96.99	0.93	0.86	183	-
--------------------------------------------------------------------------------
[2024-01-24 11:51:50,084] [INFO] GTDB search result was written to GCF_900458505.1_51395_E01_genomic.fna/result_gtdb.tsv
[2024-01-24 11:51:50,087] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:51:50,090] [INFO] DFAST_QC result json was written to GCF_900458505.1_51395_E01_genomic.fna/dqc_result.json
[2024-01-24 11:51:50,090] [INFO] DFAST_QC completed!
[2024-01-24 11:51:50,091] [INFO] Total running time: 0h0m44s