[2024-01-25 18:15:05,323] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:15:05,324] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:15:05,324] [INFO] DQC Reference Directory: /var/lib/cwl/stg48069d12-5666-4bd3-ab33-c2347e4835c3/dqc_reference
[2024-01-25 18:15:06,441] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:15:06,442] [INFO] Task started: Prodigal
[2024-01-25 18:15:06,442] [INFO] Running command: gunzip -c /var/lib/cwl/stg475c5484-4c09-454e-beab-8985f1d013bf/GCF_900458665.1_50305_H01_genomic.fna.gz | prodigal -d GCF_900458665.1_50305_H01_genomic.fna/cds.fna -a GCF_900458665.1_50305_H01_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:15:10,571] [INFO] Task succeeded: Prodigal
[2024-01-25 18:15:10,571] [INFO] Task started: HMMsearch
[2024-01-25 18:15:10,571] [INFO] Running command: hmmsearch --tblout GCF_900458665.1_50305_H01_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg48069d12-5666-4bd3-ab33-c2347e4835c3/dqc_reference/reference_markers.hmm GCF_900458665.1_50305_H01_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:15:10,778] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:15:10,779] [INFO] Found 6/6 markers.
[2024-01-25 18:15:10,800] [INFO] Query marker FASTA was written to GCF_900458665.1_50305_H01_genomic.fna/markers.fasta
[2024-01-25 18:15:10,803] [INFO] Task started: Blastn
[2024-01-25 18:15:10,803] [INFO] Running command: blastn -query GCF_900458665.1_50305_H01_genomic.fna/markers.fasta -db /var/lib/cwl/stg48069d12-5666-4bd3-ab33-c2347e4835c3/dqc_reference/reference_markers.fasta -out GCF_900458665.1_50305_H01_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:15:11,380] [INFO] Task succeeded: Blastn
[2024-01-25 18:15:11,382] [INFO] Selected 10 target genomes.
[2024-01-25 18:15:11,383] [INFO] Target genome list was writen to GCF_900458665.1_50305_H01_genomic.fna/target_genomes.txt
[2024-01-25 18:15:11,384] [INFO] Task started: fastANI
[2024-01-25 18:15:11,384] [INFO] Running command: fastANI --query /var/lib/cwl/stg475c5484-4c09-454e-beab-8985f1d013bf/GCF_900458665.1_50305_H01_genomic.fna.gz --refList GCF_900458665.1_50305_H01_genomic.fna/target_genomes.txt --output GCF_900458665.1_50305_H01_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:15:17,621] [INFO] Task succeeded: fastANI
[2024-01-25 18:15:17,621] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg48069d12-5666-4bd3-ab33-c2347e4835c3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:15:17,622] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg48069d12-5666-4bd3-ab33-c2347e4835c3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:15:17,629] [INFO] Found 9 fastANI hits (3 hits with ANI > threshold)
[2024-01-25 18:15:17,629] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:15:17,629] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Staphylococcus petrasii	strain=NCTC13835	GCA_900458665.1	1276936	1276936	type	True	100.0	839	841	95	conclusive
Staphylococcus petrasii	strain=CCM 8418	GCA_004684865.1	1276936	1276936	type	True	99.9802	821	841	95	conclusive
Staphylococcus petrasii	strain=CCUG 62727	GCA_002902565.1	1276936	1276936	type	True	99.9109	759	841	95	conclusive
Staphylococcus croceilyticus	strain=CCM 8421	GCA_004684875.1	319942	319942	type	True	94.1399	717	841	95	below_threshold
Staphylococcus croceilyticus	strain=CCUG 62728	GCA_002902575.1	319942	319942	type	True	94.0984	681	841	95	below_threshold
Staphylococcus taiwanensis	strain=NTUH-S172	GCA_020544305.1	2750012	2750012	type	True	82.7651	614	841	95	below_threshold
Staphylococcus capitis subsp. capitis	strain=DSM 20326	GCA_025272975.1	72758	29388	type	True	80.2764	414	841	95	below_threshold
Staphylococcus aureus	strain=PartF-Saureus-RM8376	GCA_022869625.1	1280	1280	type	True	79.653	331	841	95	below_threshold
Staphylococcus aureus	strain=NCTC 08532	GCA_002902205.1	1280	1280	type	True	79.2516	332	841	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:15:17,630] [INFO] DFAST Taxonomy check result was written to GCF_900458665.1_50305_H01_genomic.fna/tc_result.tsv
[2024-01-25 18:15:17,631] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:15:17,631] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:15:17,631] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg48069d12-5666-4bd3-ab33-c2347e4835c3/dqc_reference/checkm_data
[2024-01-25 18:15:17,632] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:15:17,658] [INFO] Task started: CheckM
[2024-01-25 18:15:17,658] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_900458665.1_50305_H01_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_900458665.1_50305_H01_genomic.fna/checkm_input GCF_900458665.1_50305_H01_genomic.fna/checkm_result
[2024-01-25 18:15:36,165] [INFO] Task succeeded: CheckM
[2024-01-25 18:15:36,166] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:15:36,186] [INFO] ===== Completeness check finished =====
[2024-01-25 18:15:36,187] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:15:36,187] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_900458665.1_50305_H01_genomic.fna/markers.fasta)
[2024-01-25 18:15:36,187] [INFO] Task started: Blastn
[2024-01-25 18:15:36,187] [INFO] Running command: blastn -query GCF_900458665.1_50305_H01_genomic.fna/markers.fasta -db /var/lib/cwl/stg48069d12-5666-4bd3-ab33-c2347e4835c3/dqc_reference/reference_markers_gtdb.fasta -out GCF_900458665.1_50305_H01_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:15:36,937] [INFO] Task succeeded: Blastn
[2024-01-25 18:15:36,940] [INFO] Selected 9 target genomes.
[2024-01-25 18:15:36,940] [INFO] Target genome list was writen to GCF_900458665.1_50305_H01_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:15:36,941] [INFO] Task started: fastANI
[2024-01-25 18:15:36,942] [INFO] Running command: fastANI --query /var/lib/cwl/stg475c5484-4c09-454e-beab-8985f1d013bf/GCF_900458665.1_50305_H01_genomic.fna.gz --refList GCF_900458665.1_50305_H01_genomic.fna/target_genomes_gtdb.txt --output GCF_900458665.1_50305_H01_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:15:42,491] [INFO] Task succeeded: fastANI
[2024-01-25 18:15:42,498] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:15:42,498] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002902565.1	s__Staphylococcus petrasii	99.9109	759	841	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.66	97.62	0.95	0.85	8	conclusive
GCA_002902575.1	s__Staphylococcus croceilyticus	94.0984	681	841	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.95	99.95	1.00	1.00	2	-
GCF_004785665.1	s__Staphylococcus pragensis	92.0253	733	841	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.69	99.09	0.98	0.94	4	-
GCF_003035445.1	s__Staphylococcus devriesei_A	82.6829	581	841	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	97.92	97.92	0.95	0.95	2	-
GCF_002902625.1	s__Staphylococcus devriesei	82.3099	554	841	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.59	98.61	0.94	0.91	9	-
GCF_006094395.1	s__Staphylococcus haemolyticus	82.2724	586	841	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.59	96.62	0.93	0.87	385	-
GCF_001224225.1	s__Staphylococcus borealis	81.9904	594	841	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.37	97.44	0.93	0.89	22	-
GCF_002901845.1	s__Staphylococcus hominis	81.289	503	841	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	97.87	96.99	0.93	0.86	183	-
GCF_900636385.1	s__Staphylococcus warneri	80.008	389	841	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.98	95.90	0.95	0.90	73	-
--------------------------------------------------------------------------------
[2024-01-25 18:15:42,499] [INFO] GTDB search result was written to GCF_900458665.1_50305_H01_genomic.fna/result_gtdb.tsv
[2024-01-25 18:15:42,500] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:15:42,502] [INFO] DFAST_QC result json was written to GCF_900458665.1_50305_H01_genomic.fna/dqc_result.json
[2024-01-25 18:15:42,503] [INFO] DFAST_QC completed!
[2024-01-25 18:15:42,503] [INFO] Total running time: 0h0m37s