[2024-01-24 14:21:50,636] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:21:50,638] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:21:50,638] [INFO] DQC Reference Directory: /var/lib/cwl/stg8ae4c4ea-0224-46f5-aa4d-6b729791f341/dqc_reference
[2024-01-24 14:21:51,987] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:21:51,988] [INFO] Task started: Prodigal
[2024-01-24 14:21:51,988] [INFO] Running command: gunzip -c /var/lib/cwl/stg3feac04b-a9b2-401d-9ae5-565f5b3b8702/GCF_901482635.1_42552_C01_genomic.fna.gz | prodigal -d GCF_901482635.1_42552_C01_genomic.fna/cds.fna -a GCF_901482635.1_42552_C01_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:21:56,499] [INFO] Task succeeded: Prodigal
[2024-01-24 14:21:56,500] [INFO] Task started: HMMsearch
[2024-01-24 14:21:56,500] [INFO] Running command: hmmsearch --tblout GCF_901482635.1_42552_C01_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8ae4c4ea-0224-46f5-aa4d-6b729791f341/dqc_reference/reference_markers.hmm GCF_901482635.1_42552_C01_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:21:56,806] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:21:56,807] [INFO] Found 6/6 markers.
[2024-01-24 14:21:56,839] [INFO] Query marker FASTA was written to GCF_901482635.1_42552_C01_genomic.fna/markers.fasta
[2024-01-24 14:21:56,840] [INFO] Task started: Blastn
[2024-01-24 14:21:56,840] [INFO] Running command: blastn -query GCF_901482635.1_42552_C01_genomic.fna/markers.fasta -db /var/lib/cwl/stg8ae4c4ea-0224-46f5-aa4d-6b729791f341/dqc_reference/reference_markers.fasta -out GCF_901482635.1_42552_C01_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:21:57,522] [INFO] Task succeeded: Blastn
[2024-01-24 14:21:57,525] [INFO] Selected 9 target genomes.
[2024-01-24 14:21:57,525] [INFO] Target genome list was writen to GCF_901482635.1_42552_C01_genomic.fna/target_genomes.txt
[2024-01-24 14:21:57,531] [INFO] Task started: fastANI
[2024-01-24 14:21:57,531] [INFO] Running command: fastANI --query /var/lib/cwl/stg3feac04b-a9b2-401d-9ae5-565f5b3b8702/GCF_901482635.1_42552_C01_genomic.fna.gz --refList GCF_901482635.1_42552_C01_genomic.fna/target_genomes.txt --output GCF_901482635.1_42552_C01_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:22:03,044] [INFO] Task succeeded: fastANI
[2024-01-24 14:22:03,045] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg8ae4c4ea-0224-46f5-aa4d-6b729791f341/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:22:03,045] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg8ae4c4ea-0224-46f5-aa4d-6b729791f341/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:22:03,053] [INFO] Found 6 fastANI hits (5 hits with ANI > threshold)
[2024-01-24 14:22:03,056] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:22:03,056] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Staphylococcus capitis	strain=NCTC11045	GCA_901482635.1	29388	29388	type	True	100.0	826	827	95	conclusive
Staphylococcus capitis subsp. capitis	strain=DSM 20326	GCA_025272975.1	72758	29388	type	True	99.9895	821	827	95	conclusive
Staphylococcus capitis subsp. capitis	strain=NCTC 11045	GCA_002902325.1	72758	29388	type	True	99.9777	788	827	95	conclusive
Staphylococcus capitis	strain=CCM 2734	GCA_014635765.1	29388	29388	type	True	99.9752	795	827	95	conclusive
Staphylococcus capitis subsp. urealyticus	strain=DSM 6717	GCA_002901925.1	74703	29388	type	True	96.654	711	827	95	conclusive
Staphylococcus taiwanensis	strain=NTUH-S172	GCA_020544305.1	2750012	2750012	type	True	79.7452	396	827	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:22:03,058] [INFO] DFAST Taxonomy check result was written to GCF_901482635.1_42552_C01_genomic.fna/tc_result.tsv
[2024-01-24 14:22:03,059] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:22:03,059] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:22:03,060] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8ae4c4ea-0224-46f5-aa4d-6b729791f341/dqc_reference/checkm_data
[2024-01-24 14:22:03,062] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:22:03,089] [INFO] Task started: CheckM
[2024-01-24 14:22:03,090] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_901482635.1_42552_C01_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_901482635.1_42552_C01_genomic.fna/checkm_input GCF_901482635.1_42552_C01_genomic.fna/checkm_result
[2024-01-24 14:22:24,633] [INFO] Task succeeded: CheckM
[2024-01-24 14:22:24,634] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:22:24,658] [INFO] ===== Completeness check finished =====
[2024-01-24 14:22:24,658] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:22:24,659] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_901482635.1_42552_C01_genomic.fna/markers.fasta)
[2024-01-24 14:22:24,659] [INFO] Task started: Blastn
[2024-01-24 14:22:24,659] [INFO] Running command: blastn -query GCF_901482635.1_42552_C01_genomic.fna/markers.fasta -db /var/lib/cwl/stg8ae4c4ea-0224-46f5-aa4d-6b729791f341/dqc_reference/reference_markers_gtdb.fasta -out GCF_901482635.1_42552_C01_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:22:25,498] [INFO] Task succeeded: Blastn
[2024-01-24 14:22:25,502] [INFO] Selected 12 target genomes.
[2024-01-24 14:22:25,502] [INFO] Target genome list was writen to GCF_901482635.1_42552_C01_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:22:25,520] [INFO] Task started: fastANI
[2024-01-24 14:22:25,521] [INFO] Running command: fastANI --query /var/lib/cwl/stg3feac04b-a9b2-401d-9ae5-565f5b3b8702/GCF_901482635.1_42552_C01_genomic.fna.gz --refList GCF_901482635.1_42552_C01_genomic.fna/target_genomes_gtdb.txt --output GCF_901482635.1_42552_C01_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:22:31,779] [INFO] Task succeeded: fastANI
[2024-01-24 14:22:31,789] [INFO] Found 12 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:22:31,790] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002902325.1	s__Staphylococcus capitis	99.9777	788	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	97.07	96.40	0.94	0.88	151	conclusive
GCF_002902725.1	s__Staphylococcus caprae	84.2897	654	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.57	98.10	0.94	0.91	18	-
GCF_900458815.1	s__Staphylococcus saccharolyticus	81.8546	511	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.11	97.94	0.98	0.95	15	-
GCF_006742205.1	s__Staphylococcus epidermidis	81.0177	490	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.01	96.59	0.94	0.83	1079	-
GCF_900636385.1	s__Staphylococcus warneri	80.78	429	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.98	95.90	0.95	0.90	73	-
GCF_003491325.1	s__Staphylococcus warneri_A	80.716	438	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.32	98.95	0.94	0.90	22	-
GCF_003970495.1	s__Staphylococcus pasteuri	80.2011	432	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.98	98.67	0.95	0.89	34	-
GCF_002902565.1	s__Staphylococcus petrasii	80.0762	370	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.66	97.62	0.95	0.85	8	-
GCF_004785665.1	s__Staphylococcus pragensis	79.9079	373	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.69	99.09	0.98	0.94	4	-
GCA_002902575.1	s__Staphylococcus croceilyticus	79.7069	374	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.95	99.95	1.00	1.00	2	-
GCF_003035445.1	s__Staphylococcus devriesei_A	79.6706	356	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	97.92	97.92	0.95	0.95	2	-
GCF_015025035.1	s__Staphylococcus sp015025035	79.4954	344	827	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.24	97.61	0.95	0.93	11	-
--------------------------------------------------------------------------------
[2024-01-24 14:22:31,791] [INFO] GTDB search result was written to GCF_901482635.1_42552_C01_genomic.fna/result_gtdb.tsv
[2024-01-24 14:22:31,792] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:22:31,796] [INFO] DFAST_QC result json was written to GCF_901482635.1_42552_C01_genomic.fna/dqc_result.json
[2024-01-24 14:22:31,796] [INFO] DFAST_QC completed!
[2024-01-24 14:22:31,796] [INFO] Total running time: 0h0m41s